Cell Counting Kit-8 (CCK-8) B34304 from Selleck Chemicals

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanySelleck Chemicals
Price $499.00Supplier Page View Company Product Page
Catalog NumberB34304
Storage3 years-20°C powder 2 years-80°C in solvent
Quantity25 mL (2500 rxns)

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Selleck Chemicals

14408 W Sylvanfield Drive

Houston, Texas TX 77014

United States

Phone: Toll Free: (877) 796-6397 (US and Canada only)
Tel: (832) 582-8158
Fax: (832) 582-8590

Email: sales@selleckchem.com

Company Profile

Website: www.selleckchem.com

(1) Lactobacillus salivarius potentiates gastrointestinal cancer immunotherapy through its metabolite chenodeoxycholic acidCell Reports MedicineSeptember 15, 2026Bohan Zhang, Yifan Zhang, Yi Cai, Siyuan Cheng, Zihan Han, Xiaochen Yin, Tong Xie, Yan Tan, Yang Feng, Yuxin Wang, et al.Kit,Dakewe,Cat#EK280HS Mouse IL-10 ELISA kit,Dakewe,Cat#A2413 Cell Counting Kit-8 (CCK-8),Selleck,Cat#B34304 ATP Assay Kit,Beyotime,Cat#S0026 NAD+/NADH Assay Kit,Beyotime,Cat#S0176S Reactive Oxygen Species

(2) Mesenchymal stem cells as cellular factories for targeted P2X7 blockade after traumatic brain injuryNeuroprotectionSeptember 3, 2026Lucia Fadon-Padilla, Chengyan Chu, Lorissa McDougall, Yajie Liang, Chinmoy Sarkar, Marta Lipinski, Tim Magnus, Miroslaw Janowski, Piotr Walczakprotocol. 2.5 Cell viability Cell viability was measured using a Cell Counting Kit‐8 (CCK‐8) assay (B34304; Selleckchem, Houston, TX, USA). In brief, the CCK‐8 reagent was diluted 1:10 in RoosterNourish™‐MSC‐XF

(3) TAB2 Causes Neuronal Damage by Aggravating Microglia-Mediated Neuroinflammation in Parkinson's DiseaseAdv Sci (Weinh)August 17, 2026Yanhao Zhao, Zimeng Huang, Jianing Gao, Aolan Li, Huizhi Wang, Yingchuan Chen, Guanyu Zhu, Xin Zhang, Yin Jiang, Moxuan Zhang, et al.Selleck Chemicals). 4.5 Cell Viability Assay Cell viability was assessed using the Cell Counting Kit‐8 (B34304; Selleck Chemicals). Briefly, cells were seeded in 96‐well plates at a density of 2 × 103 cells/well

(4) Machine learning algorithms develop a tumor-educated platelets-related gene signature to predict colorectal cancer prognosis and therapy responseiScienceJuly 17, 2026Yueqiong Lao, Jie Yang, Minli Hu, Jing Li, Weikang Xu, Jiahui Xu, Huan Wang, Hechenhao Jiang, Zhihao Pei, Xinyi Qiu, et al.parallel. Cell viability and colony formation assays Cell viability was measured using a CCK-8 kit (#B34304, Selleck, USA), and colony formation ability was estimated by counting the number of colonies after

(5) A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repairBioactive MaterialsJuly 1, 2026Xueheng Sun, Shangyu Xie, Ya Fang, Deteng Zhang, Wenhao Wang, Zeyue Sun, Jiaju Lu, Xiaokun Yue, Weize Kong, Dehao Fu, et al.Penicillin-streptomycin (SV30010) was obtained from HyClone Laboratories, Inc (Logan, Utah, USA). CCK-8 (B34304), and SYBR Green (B21202) were purchased by Selleck Chemicals LLC (Houston, TX, USA). Live/dead staining

(6) Integrin α6-targeted photodynamic therapy potentiates PD-1 blockade in MSS colorectal cancerJournal of NanobiotechnologyJune 17, 2026Ting Hu, Qian-Kun Shi, Qiao-Li Wang, Ze-Rong Huang, Wan-Qi Chen, Lin-Na Luo, Kai-Li Xie, Fang Hu, Jie Yang, Jian-Jun Li, et al.software. Cell viability assay (CCK-8) Cell viability was evaluated using a CCK-8 kit (Selleck Chemicals, #B34304). CRC cells (CT26, HCT116, SW480, and MC38) and normal colon epithelial cells (FHC and NCM460) were

(7) Snhg18 promotes hypoxic pulmonary hypertension by enhancing glycolysisRespiratory ResearchMay 18, 2026Tianyu Qu, Kai Ma, Qiang Du, Chun Zhang, Ziling Shen, Enze Wang, Zhixuan Chen, Pingsheng Chen, Yingying Liu, Ruifeng Zhanghypoxic (3% O2) or normoxic (21% O2) conditions for 24 h. Then, 10 µL Cell Counting Kit-8 reagent (Cat. B34304, Selleck) was added to each well. After incubation at 37 °C for 2 h, the absorbance at 450 nm was determined

(8) Hyperglycemia- induced innate immune tolerance involves the metabolic and epigenetic rewiring in human alveolar macrophagesFront ImmunolMay 7, 2026Jiang Wang, Na Yuan, Bin Wang, YongHui Qiu, Bo Wang, LiXin Xieviability test Cell viability was assessed using the Cell Counting Kit−8 (CCK−8) assay (Selleck, Cat# B34304, USA). Primary human AMs were seeded into 96−well plates at a density of 6 × 104 cells/well and cultured

(9) RFXANK: A Novel Immune-Related Biomarker for Hepatocellular CarcinomaGenes (Basel)March 31, 2026Taimei Qu, Lv Tian5′-CAGTGGCGTCCCTCCATTC-3′. 2.11. Cell Proliferation Assay Cell proliferation was assessed using the Cell Counting Kit-8 (B34304, Selleckchem, Houston, TX, USA). In brief, cells were inoculated into 96-well microplates at an initial

(10) An Analysis of G3BP2 in Non-Small Cell Lung CancerCancers (Basel)March 17, 2026Leela S. S. Bandi, Leah Timon, Elena O'Toole, Diarmuid O'Connor, Kristen Andersen, Bashir M. Mohamed, Siobhan Nicholson, Gerard J. Fitzmaurice, Ronan Ryan, Vincent Young, et al.further 48 h. Cellular viability was assessed using a CCK-8 assay (Selleckchem, Houston, TX, USA Cat# B34304) according to the manufacturer’s protocol. 2.12. Availability of Data and Materials The data that

(11) The segmented flavivirus ALSV-encoded nucleoprotein VP2 inhibits type I interferon production by targeting RIG-IMicrobiology SpectrumMarch 3, 2026Mingming Pan, Zhixia Song, Mengmeng Wang, Mengru Zhao, Shu Fang, Fangyu Jin, Qianqian Tan, Wenbo Xu, Lihe Che, Nan Liu, et al.after cell growth at 24 h and incubated for 12 h. Then, the cells with CCK-8 reagent (Selleck, cat#B34304) were incubated for 1 h, and the absorbance at 450 nm was measured using a microplate reader. The 50%

(12) Lactate derived from cancer-associated fibroblasts promotes alternative splicing and castration resistance in prostate cancerScience AdvancesJanuary 16, 2026Diwei Zhao, Zijun Mo, Tianyou Zhang, Xinyang Cai, Zhenyu Yang, Dong Chen, Junliang Zhao, Yuanwei Li, Fangjian Zhou, Zhen Li, et al.assays For cell proliferation, CCK-8 assays were conducted following the manufacturer’s instructions (B34304, Selleck). C4-2 and LNCaP cells were plated in 96-well plates at a density of 3000 cells per well and

(13) HMOX1 drives dihydroartemisinin-sensitized ferroptosis antagonized by mitochondrial fusioniScienceJanuary 16, 2026Zi-Jie Deng, Jing Zhang, Zhang-Zhong Yang, Qing-Zhang Tuo, Peng Leisalt,Sigma-Aldrich,K1875 Rotenone,Selleck,S2348 H2O2,Macklin,H792072 FINO2,Selleck,E1244 BSO,Selleck,S9728 Erastin,Selleck,S7242 Ammonium iron(II) sulfate hexahydrate (FAS),Sigma-Aldrich,215406 Cell Counting Kit-8 (CCK-8),Selleck,B34304 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Live and Dead Cell Assay

(14) Targeted demethylation of the BRD7 promoter based on CRISPR/dCas9 system inhibits the malignant progression of nasopharyngeal carcinomaClinical and Translational MedicineJanuary 9, 2026Jianxia Wei, Yumei Duan, Changning Xue, Lemei Zheng, Qingqing Wei, Zubing Wu, Huizhen Xin, Ting Zeng, Hongyu Deng, Songqing Fan, et al.100 µL culture medium per well were plated in a 96‐well plate. Ten microlitre CCK‐8 reagent (Cat No. B34304, Selleck) was introduced and incubated at 37°C for 2–4 h at specified designated time intervals (0,

(15) GYY4137 protects against type 2 diabetes mellitus-associated myocardial autophagy by suppressing FOXO1 signal pathwayAnimal Cells and SystemsDecember 31, 2025Gaofeng Zhu, Xiaoyong Li, Qinyuan Gao, Yuanjun Wang, Jiajie Li, Zena Huang, Yan LinPBS. Then the cells were incubated with 10 μL CCK-8 test solution (Selleck, Shanghai, China, Cat. No: B34304) and 90 μL DMEM at 37℃ for 2 h. The optical density (OD) was determined by measuring the absorbance

(16) TRIM25 degrades BRD7 protein stability through the ubiquitin proteasome pathway to promote breast cancer progression and paclitaxel resistance by activating YB1/Bcl-2 transcription axisCell Death & DiseaseNovember 28, 2025Changning Xue, Qingqing Wei, Mengna Li, Jianxia Wei, Lemei Zheng, Zubing Wu, Huizhen Xin, Yumei Duan, Songqing Fan, Wei Xiong, et al.proliferation assay, the cell viability of breast cancer cells was quantified by Cell Counting Kit-8 (CCK-8, B34304, Selleck, Houston, TX, USA). Briefly, 1000 cells/100 μl of DMEM medium were plated in each well of a

(17) Engineering Lipid-Polymer Nanoparticles for siRNA Delivery to Cancer CellsPharmaceuticals (Basel)June 10, 2025Arthur Manda, Abdulelah Alhazza, Hasan Uludağ, Hamidreza Montazeri Aliabadi1027292) were purchased from Qiagen (Germantown, MD, USA). The cell counting 8 (CCK-8) (catalogue no. B34304) kit was provided by Selleck Chemicals LLC (Houston, TX, USA). Cell culture reagents, plates, phosphate

(18) Lovastatin Targets the USP14-Survivin Axis to Suppress Triple-Negative Breast Cancer via Ubiquitin-Mediated Proteasomal DegradationCellsMay 31, 2025Li Zhou, Chanjuan Zheng, Siyu Ding, Zhiyu Wang, Yiyuan Yang, Yian Wang, Guangchun He, Shujun Fu, Xiyun Dengincluded to assess solvent effects. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, #B34304, Selleck, Houston, TX, USA) according to the manufacturer’s protocol. Three independent replicates were

(19) cIAP2-mediated IGF2BP2 ubiquitination and degradation regulate cardiomyocyte apoptosis via stabilizing m6A-modified BAX mRNA in myocardial infarctionCell Biol ToxicolMay 30, 2025Cong Wang, Jijia Liu, Xuyang Hou, Qing Guan, Huiling Zhou, Yong Luo, Wancun Jin, Fan Bai, Lijun Liu, Jian Wang, et al.seeded in 96-well plates and treated with normoxia or OGD for 6 h. After treatment, 10 μL CCK-8 (cat. #B34304, Selleck Chemicals, USA) was added and incubated for another 1 h. Then the absorbance at 450 nm was

(20) Natural compound rosmarinic acid displays anti-tumor activity in colorectal cancer cells by suppressing nuclear factor-kappa B signalingWorld Journal of Clinical OncologyMay 24, 2025Wen-Yue Liu, Han Wang, Xin Xu, Xuan Wang, Kun-Kun Han, Wen-Dao You, Yili Yang, Tao Zhangusing the Cell Counting Kit-8 (CCK-8) reagent according to the manufacturer’s instruction (Selleck, #B34304). Quantitative real-time polymerase chain reaction Quantitative real-time polymerase chain reaction

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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