Cell Counting Kit-8 (CCK-8) B34302 from Selleck Chemicals

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanySelleck Chemicals
Price $139.00Supplier Page View Company Product Page
Catalog NumberB34302
Storage3 years-20°C powder 2 years-80°C in solvent
Quantity5 mL (500 rxns)

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Selleck Chemicals

14408 W Sylvanfield Drive

Houston, Texas TX 77014

United States

Phone: Toll Free: (877) 796-6397 (US and Canada only)
Tel: (832) 582-8158
Fax: (832) 582-8590

Email: sales@selleckchem.com

Company Profile

Website: www.selleckchem.com

(1) Wt1 facilitates visceral beige fat formation to combat abdominal obesityAdipocyteDecember 31, 2026Lin-Fei He, Zhi-Mei Liao, Jun-Ling Chen, Zhi-Chun Yangculture medium for a further four days. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, B34302, Selleck, Houston, TX, USA). Cells were seeded in 96-well plates and starved for 24 hours. After drug

(2) Astragaloside IV Alleviates Osteoarthritis by Upregulating ETS2: A Bioinformatics and Experimental StudyJ Inflamm ResJuly 7, 2026Lingxing Li, Fulai Zhao, Wenyun Kui, Wenlan Du, Youwei Wang, Shuang Liu, Yongpeng Xue, Jiafan Yang, Weiwei Da, Xiaofeng Li, et al.of AS-IV on the viability of primary chondrocytes was evaluated using a CCK-8 assay kit (Selleck, #B34302) in accordance with the manufacturer’s instructions. In brief, primary chondrocytes were plated into

(3) Penfluridol Triggers GSDME-Mediated Immunogenic Pyroptosis to Potentiate Antitumor ImmunotherapyAdv Sci (Weinh)July 3, 2026Linfeng Li, Daishi Li, Danyao Chen, Yating Dian, Lei Yao, Hui Su, Ziyu Guo, Deze Zhao, Zihua Wu, Furong Zeng, et al.S1). 4.3 Cell Viability and Death Analysis We measured cell viability with the CCK‐8 assay (Bimake, B34302), following a previously reported protocol [8]. To quantify cell death, cells were stained with propidium

(4) Inhibition of PKCγ phosphorylation protects against cerebral ischemia-reperfusion injuryRedox BiologyJuly 1, 2026Chenchen Li, Jinlun Chen, Xiangbin Ouyang, Ruijia Duan, Yijin Kuang, Yaohui He, Jieqiong Tan, Liuwang Zenggrowth rate and size. After treatment, the medium was removed and CCK8 (Selleck Chemicals, TX, USA, #B34302) was added to the plates according to the instructions. After incubation at 37 °C for 1 h, optical density

(5) Virus-host interactome reveals host cellular pathways perturbed by tick-borne encephalitis virus infectioniScienceJune 19, 2026Liyan Sui, Ying-Hua Zhao, Heming Wang, Hongmiao Chi, Hanxi Xie, Tian Tian, Wenfang Wang, Mucuo Zhu, Naicui Zhai, Zhixia Song, et al.was performed in triplicate. Following the incubation period, 10 μL of CCK8 solution (based on WST-8, B34302, Selleck) was added to each well and incubated for 4 h in a cell culture incubator. The absorbance value

(6) Dynamic microenvironment-regulated hydrogels releasing celastrol lead to urethral scarless repairMaterials Today BioApril 1, 2026Yangwang Jin, Fei Qin, Ranxing Yang, Wenzhuo Fang, Kaile Zhang, Meng Liu, Ming Yang, Ying Wang, Qiang FuTGF-β1 + 0.1 μM Cel, while control groups received untreated medium. After 48 h treatment, 10 μL CCK-8 (B34302, Selleckchem.com) was added per well, followed by 2 h incubation at 37 °C. Absorbance was measured at

(7) Identification of STMN1 as a lactylation‑related driver of lung cancer progression using Mendelian randomizationMolecular Medicine ReportsMarch 30, 2026Yifan Cai, Yucheng Zhong, Honglin Wang, Shuang Zhu, Fang Huang, Qiuyue Zhang, Shaobo Hucolony formation assays Cell viability was evaluated using the Cell Counting Kit-8 (CCK-8; cat. no. B34302; Selleck Chemicals). Briefly, DMS114 and H196 cells were seeded onto 96-well culture plates at a density

(8) Preoperative exercise induces anti-tumor Kupffer cells to prevent surgical stress-promoted colorectal cancer liver metastasisCell Reports MedicineFebruary 17, 2026Yunwei Zhang, Yiyu Zhang, Chengli Shen, Jiye Li, Han Wang, Ahmad Hamad, Chunyan Cao, Mohamad El Moheb, Yu Wang, Yujia Xia, et al.MicroBead Kit, human",Miltenyi Biotec,Cat# 130-124-420 Cell Counting Kit-8 (CCK-8),Selleck Chemicals,Cat# B34302 ,, Deposited data,Deposited data,Deposited data ,, Raw and processed scRNA-seq data from sedentary

(9) Targeted Induction of Cancer Cell Necroptosis Potentiates Anti-PD-1 Immunotherapy via CD80 ActivationInt J Biol SciJanuary 22, 2026Xu Zhang, Detian Zhang, Zhe Zhou, Waner Liu, Susi Zhu, Siyu Xiong, Xiang Chen, Cong Pengthe OD value at a wavelength of 450 nm after incubation with a Cell Counting Kit 8 (CCK-8) solution (B34302; Selleck, USA). Each experiment was conducted independently at least three times. The half inhibitory

(10) Inhibition of HER2 signaling and breast cancer cell growth with a novel antibody targeting HER2 ECD III/IVPLOS OneJanuary 15, 2026Chunchun Liu, Yulei Ren, Xuan Luo, Peiyun Zhou, Xiangjuan Du, Qianmin Wang, Xue Han, Yanhui Xu, Ping Wang, Dan Zhao, et al.Following the treatment period, cell viability was assessed using the Cell Counting Kit-8 (CCK-8, Selleck, B34302) according to the manufacturer’s instructions. Absorbance was measured at 450 nm using a Synergy H1

(11) Collateral metabolic vulnerabilities unveiled by loss of isozyme diversity in breast cancerGenome MedicineDecember 12, 2025Rui Ding, Tian-Jian Yu, Yi-Zhou Jiang, Yi Xiao, Zhi-Ming Shaosignals. Cell proliferation and colony formation assays A Cell Counting Kit-8 (CCK-8) (Selleck, cat. no. B34302) was utilized to assess cell proliferation. For the cell viability assays, the cells were plated in

(12) Vine Tea Combined with a Lidocaine Microneedle Patch for Enhanced Treatment of PsoriasisACS OmegaNovember 4, 2025Ziyan Chen, Ying Wang, Ye Kai, Xuanjie Huang, Mingyi Tan, Huayi Wu, Wangqing Chen, Wu Zhu, XiaoWei Liang, Peipei Wang, et al.Biocompatibility of the MN Patch Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, Selleck, B34302) assay. HaCaT cells (1 × 10̂4 cells/well) and NIH 3T3 cells (8 × 10̂3 cells/well) were seeded in 96-well

(13) Epithelial‐Mesenchymal Transition States and Metabolic Reprogramming Related Signatures Predict Prognosis and Therapeutic Responses in HER2 ‐Positive Breast CancerJournal of Clinical Laboratory AnalysisNovember 3, 2025Yuhui Zheng, Yinduo Zeng, Mengping Xi, Guoqiang Zhu, Jinyu Yang, Peng Cui, Guoqiang Wang, Yuzi Zhang, Chunwei Xu, Wenxian Wang, et al.proliferation was analyzed for the following 4 days after planting using Cell Counting Kit‐8 (Selleck, Cat#:B34302) following the manufacturer's instructions. Cell viability was assessed by measuring OD values. Absorbance

(14) Targeting CLEC4E in immunosuppressive tumour-associated macrophages via BET inhibitionClin Transl MedOctober 15, 2025Mengting Liao, Kexin Long, Liang Dong, Zhuo Li, Wenhua Wang, Rui Hu, Yangyi Zhang, Juan Su, Wu Zhu, Xiang Chen, et al.Cell counting kit‐8 assay Cell proliferation was analysed by cell counting kit‐8 (CCK‐8, Selleck, B34302) according to the manufacturer's protocols. Cells were seeded at 5000 cells per well with IMDM in 96‐well

(15) SENP3 protects hepatocyte from pyroptosis during acute liver injury through deSUMOylation of HNRNPLiScienceAugust 15, 2025Xinyuan Xiong, Yang Zhi, Nan Yang, Wenzhen Zhao, Shu Wang, Huiqin Zhu, Jieting Tang, Jing Yi, Xuxu Sun, Jie Yang20 μM Nigericin (MedChemExpress, HY-127019) was added to each well for 24 h. CCK-8 reagent (Selleck, B34302) was added and incubated for 2 h. The absorbance was measured at 450 nm by a microplate reader. Propidium

(16) Crizotinib: A Novel Strategy to Reverse Immunosuppression in Melanoma by Targeting Lactate TransportMedComm (2020)August 1, 2025Zhou Z, Zhang X, Zhu S, Liu W, Guo Y, Xiong S, Peng C, Chen X.in 96‐well plates and treated with crizotinib or the control. Then 10 µL of CCK‐8 reagent (Selleck, B34302, USA) was added, followed by incubation at 37°C for 2 h. Absorbance was measured using a Cytation1 instrument

(17) Hepatic ferroptosis induced by Clonorchis sinensis exacerbates liver fibrosisPLoS neglected tropical diseasesJune 1, 2025Zhang H, Wang X, Zhang X, Ma Y, Bao P, Yu Y, Wang Y, Gong P, Zhang N, Lee SO, et al.USA) was set as the positive control. Cell viability was measured by using the CCK-8 assay (Selleck, B34302, USA). 4.5 Animal experiment The mice were evenly and randomly allocated into two distinct groups:

(18) TRIM25 facilitates ferroptosis in ovarian cancer through promoting PIEZO1 K63-linked ubiquitination and degradationTransl OncolApril 18, 2025Ya Li, Fei Zhou, Zhengmei Xu(C0105, Beyotime, Nanjing, China). Cell viability assay Utilize the Cell Counting Kit-8 (CCK-8) (B34302, Bimake) in accordance with the manufacturer's guidelines. The detailed procedure is as follows: Inoculate

(19) Topical TYK2 inhibitor ameliorates psoriasis-like dermatitis via the AKT-SP1-NGFR-AP1 pathway in keratinocytesClinical and translational medicineMarch 1, 2025Fang Z, Jiang R, Wang Y, Chen W, Chen X, Yin M.overexpression of target genes. 2.6 CCK‐8 assay Cell proliferation was evaluated with the CCK‐8 assay (cat. B34302, Selleck). Briefly, KCs with the specific treatment were seeded in 96‐well plates at an appropriate

(20) A Silk Fibroin Nanoparticle Hydrogel Loaded With NK1R Antagonist Has Synergistic Anti‐Inflammatory and Reparative Effects on Dry Eye DiseaseAdvanced ScienceFebruary 22, 2025Bo Gong, Yi Liu, Huan Li, Xueming Ju, Dongfeng Li, Yuhao Zou, Xiaoxin Guo, Kai Dong, Jialing Xiao, Weijia Wu, et al.Cytotoxicity Assays The biocompatibility of the materials was assessed using the CCK‐8 assay (Selleck, B34302, China). HCECs were purchased from Procell (CL‐0743, Wuhan, China). HCECs were analyzed using DMEM/F12

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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Cell Counting Kit-8 (CCK-8) from Selleck Chemicals

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