TruSeq DNA PCR-Free High Throughput Library Prep Kit 20015963 from Illumina

Product Specs
ItemTruSeq DNA PCR-Free High Throughput Library Prep Kit
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20015963
ApplicationsWhole-genome sequencing
ReactivityHuman, Mouse, Rat, Any
TypeDNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Murine osteosarcoma recapitulates the driver landscape and genomic complexity of osteosarcoma evolution in humansbioRxivMay 5, 2026Geoffrey A. Smith, Ianthe A.E.M van Belzen, Mathieu Epinette, Emilia Herdes, Kim L. Mercer, Charlotte G. Butterworth, Alistair G. Rust, Adrienne M. Flanagan, Matthew G. Jones, Isidro Cortes-Ciriano, et al.to prepare sequencing libraries with the TruSeq DNA PCR-Free High Throughput Library Prep Kit (cat. #20015963, Illumina) as per the manufacturer’s instructions, with the exception of 2 samples with low DNA quantities

(2) Maternal Immune Activation Disrupts Epigenomic and Functional Maturation of Cortical Excitatory NeuronsbioRxivApril 12, 2026Chi-Yu Lai, Jessica Arzavala, Antonio Pinto-Duarte, Shiyuan Wang, Junhao Li, Hanqing Liu, Julia Osteen, Rosa Gomez Castanon, Joseph Nery, Susan B. Powell, et al.poly-adenylated mRNA transcripts using Truseq Stranded mRNA High Throughput Library Prep kit (Illumina, 20015963) with TruSeq RNA CD Indexes (Illumina, 20015949). Libraries were sequenced using Illumina NextSeq500,

(3) Mechanisms of Bacillus amyloliquefaciens on alleviating lipopolysaccharide-induced oxidative stress in broilersAnimal NutritionMarch 1, 2026Xing Chen, Aijuan Zheng, Zhimin Chen, Peilong Yang, Jiang Chen, Zhiheng Zou, Guohua Liu[5′-GGACTACHVGGGTWTCTAAT-3’]), and library construction was carried out using the Tru Seq DNA PCR-Free Sample Preparation Kit (20015963, Illumina, Inc., San Diego, CA, USA). Sequencing was conducted on an Illumina HiSeq2500 platform for

(4) Chloroplast genome sequencing in winged bean (Psophocarpus tetragonolobus L.) and comparative analysis with other legumesBMC Plant BiologyJanuary 14, 2026Nikhil Kumar Singh, Binay K. Singh, Anupama Gidhi, Harsha Srivastava, Avinash Pandey, Sudhir Kumar, A. Pattanayak, V. P. Bhadana, Sujay Rakshit, Kishor U. Tribhuvanwhole-genome sequencing library was constructed using the TruSeq DNA PCR-Free Library Prep Kit (Illumina, catalog no. 20015963) following the manufacturer’s instructions. The prepared library was quantified using a Qubit 3 fluorometer

(5) Haplotype-resolved genomics identifies cyp19a1a as a candidate master sex-determining gene in golden trevally (Gnathanodon speciosus)iScienceOctober 17, 2025Bin Fan, Jiamin Guo, Caixia Lei, Sen Yang, Zining Meng, Junyao Peng, Yongjian Yang, Yubang Shen, Yuanyou Li, Le Wang,Thermo Fisher,Cat#15596018 PCR master mix,Sangon Biotech,Cat#B639295 Truseq DNA PCR free Library Preparation Kit,Illumina,Cat#20015963 TruSeq Stranded mRNA Library Prep Kit,Illumina,Cat#RS1222001 SYBR® Green Real-time PCR Master Mix,TOYOBO,Cat#QPK-101 Dual-Luciferase® Reporter Assay System

(6) Complete mitochondrial genome of Leucopsarion petersii (Gobiiformes, Gobiidae) assembled from next-generation sequencing dataMitochondrial DNA B ResourOctober 3, 2025Jeong-Soo Gim, Dong-Hyun Hong, Jeong-An Gim, Maurice Lineman, Gea-Jae Joo, Hyunbin Jo& Tissue Kit (Qiagen, Hilden, Germany). Library preparation (Illumina TruSeq DNA PCR-free; Cat. No. 20015963), genome assembly (SPAdes, V 3.12), and data preparation for DNA sequencing (101 bp paired-end reads

(7) APOBEC3A drives deaminase mutagenesis in human gastric epitheliumGenome ResearchOctober 1, 2025Yohan An, Ji-Hyun Lee, Joonoh Lim, Jeonghwan Youk, Seongyeol Park, Ji-Hyung Park, Kijong Yi, Taewoo Kim, Chang Hyun Nam, Won Hee Lee, et al.(QIAGEN, #69506), and libraries were constructed with TruSeq DNA PCR-Free Library Prep kits (Illumina, #20015963). Whole-genome sequencing was performed on the NovaSeq 6000 with a mean 30× depth of coverage. Adapter

(8) Exercise-Induced Changes in Enterohepatic Communication Are Linked to Liver Steatosis ResolutionNutrientsSeptember 15, 2025Yong Zou, Jie Xia, Sen Zhang, Yingjie Guo, Weina Liu, Zhengtang QiQiagen, Hilden, Germany) of target fragments. Sequencing libraries were prepared using an Illumina kit (20015963, Illumina, San Diego, CA, USA) and quantified by Qubit and qPCR before NovaSeq6000 sequencing. Raw sequencing

(9) Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patientsNPJ Biofilms MicrobiomesAugust 9, 2025Jing Yang, Yi Zhou, Aiping Du, Zhongwei Zhang, Bo Wang, Yongming Tian, Huan Liu, Lin Cai, Fang Pang, Yumei Li, et al.performed using the TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA, Cat#20015963). After being quantified by using Qubit (Thermo Fisher), the samples were subjected to sequencing using

(10) The human gut microbiome and its metabolic pathway dynamics before and during HIV antiretroviral therapyMicrobiology SpectrumAugust 5, 2025Oumar Dolo, Fousseini Coulibaly, Anou M. Somboro, Shan Sun, Modibo Diarra, Aminata Maiga, Soungou Bore, Djeneba B. Fofana, Anne-Genevieve Marcelin, Brehima Diakite, et al.microbiota. Library preparation was conducted using the Illumina DNA PCR-Free Library Prep Kit (Cat. No. 20015963), which minimizes amplification bias, thus preserving the original composition of microbial communities.

(11) Regulatory orchestration of FK506 biosynthesis in Streptomyces tsukubaensis NRRL 18488 revealed through systematic analysisiScienceJune 20, 2025Namil Lee, Woori Kim, Ji Hun Kim, Yongjae Lee, Soonkyu Hwang, Gahyeon Kim, Hyeseong Kim, Qingyun Dan, Matthias Schmidt, Yeo Joon Yoon, et al.Wizard Genomic DNA Purification Kit,Promega,Cat#A1120 SMRTbell™ Template Prep Kit,Pacific Biosciences,Cat#100-259-100 TruSeq DNA-Free LT kit,Illumina,Cat#20015963 ,, Deposited data,Deposited data,Deposited data ,, Streptomyces tsukubaensis NRRL 18488 genome and

(12) Error-corrected flow-based sequencing at whole-genome scale and its application to circulating cell-free DNA profilingNature MethodsMay 1, 2025Alexandre Pellan Cheng, Adam J. Widman, Anushri Arora, Itai Rusinek, Aaron Sossin, Srinivas Rajagopalan, Nicholas Midler, William F. Hooper, Rebecca M. Murray, Daniel Halmos, et al.libraries were prepared on 1μg of DNA using the TruSeq DNA PCR-Free Library Preparation Kit (Illumina, cat#20015963), with one additional bead cleanup performed after end-repair and after adapter ligation. DNA was quantified

(13) Baseline abundance of Akkermansia muciniphila and Bacteroides acidifaciens in a healthy state predicts inflammation associated tumorigenesis in the AOM/DSS mouse modelSci RepApril 10, 2025Kseniya M. Achasova, Olga A. Snytnikova, Kseniya E. Chanushkina, Maryana V. Morozova, Yuri P. Tsentalovich, Elena N. Kozhevnikovaproducts were purified and libraries were prepared using TruSeq® DNA PCR-Free Sample Preparation Kit (20015963, Illumina, USA) according to the manufacturer’s recommendations. Libraries were pooled in equal ratios

(14) Evaluation of whole genome sequencing utility in identifying driver alterations in cancer genomeScientific ReportsOctober 12, 2024Takeshi Nagashima, Ken Yamaguchi, Kenichi Urakami, Yuji Shimoda, Sumiko Ohnami, Keiichi Ohshima, Tomoe Tanabe, Akane Naruoka, Fukumi Kamada, Masakuni Serizawa, et al.(Illumina). Library construction was performed using a TruSeq DNA PCR-Free High Throughput Library Prep Kit (20015963; Illumina) from 1 µg DNA, according to the manufacturer’s instructions. Bcl2fastq v2.20 (Illumina) was

(15) Potential Plasma Proteins (LGALS9, LAMP3, PRSS8 and AGRN) as Predictors of Hospitalisation Risk in COVID-19 PatientsBiomoleculesSeptember 17, 2024Thomas McLarnon, Darren McDaid, Seodhna M. Lynch, Eamonn Cooper, Joseph McLaughlin, Victoria E. McGilligan, Steven Watterson, Priyank Shukla, Shu-Dong Zhang, Magda Bucholc, et al.Analysis Whole-genome library preparation was performed using the Illumina TruSeq PCR Free Library Prep protocol (20015963) using a sample volume input of 1 µg (n = 483 passed QC). Library preparation was automated and processed

(16) GoEnrich: creating high quality genomic DNA resources from limited voucher specimen tissues or museum specimens of at-risk species for conservation-friendly use in the validation of environmental DNA assaysBMC Research NotesSeptember 10, 2024Mark Louie D. Lopez, Matthew T. Bonderud, Isabel G. Ma, Vanessa C. Thompson, Caren C. Helbingfor library preparation and whole genome sequencing. The TruSeq DNA PCR-free library prep kit (Cat. 20015963, Illumina Inc., California, US) was used for library preparation using the Microlab NIMBUS liquid handling

(17) Genome sequence analyses identify novel risk loci for multiple system atrophyNeuronJuly 3, 2024Ruth Chia, Anindita Ray, Zalak Shah, Jinhui Ding, Paola Ruffo, Masashi Fujita, Vilas Menon, Sara Saez-Atienzar, Paolo Reho, Karri Kaivola, et al.AS1610 PicoGreen dsDNA assay,Thermo Fisher,Catalog # P7589 TruSeq PCR-free Library Prep Kit,Illumina,Catalog # 20015963 HiSeq X Ten Reagent Kit (v.2.5 chemistry),Illumina,Catalog # FC-502-2501 Deposited Data,Deposited

(18) Monobutyrin Can Regulate the Gut Microbiota, Which Is Beneficial for the Development of Intestinal Barrier Function and Intestinal Health in Weaned MiceNutrientsJune 27, 2024Wang H, Qiu J, Zhou M, Luo Y, Li X, Wang M.Germany). Sequencing libraries were generated using a TruSeq® DNA PCR-Free Sample Preparation Kit (Cat# 20015963, Illumina, San Diego, CA, USA) and qualified with a Qubit@ 2.0 Fluorometer (Thermo, Waltham, MA, USA)

(19) Correlation between intestinal flora and GLP-1 receptor agonist dulaglutide in type 2 diabetes mellitus treatment—A preliminary longitudinal studyiScienceMay 17, 2024Lei Liang, XiaoYun Su, Yaxin Guan, Bin Wu, Xuxiang Zhang, Xin Nianassays,Critical commercial assays ,, TruSeq DNA PCR-Free Sample Preparation kit,"Illumina, San Diego",Cat: 20015963 Phusion@High-fidelity PCR Master Mix with GC Buffer,New England Biolabs,Cat: M0532s ,, Deposited

(20) Emergence and genomic characterization of Proteus mirabilis harboring blaNDM-1 in Korean companion dogsVeterinary ResearchApril 9, 2024Su Min Kyung, Jun Ho Lee, Eun-Seo Lee, Xi-Rui Xiang, Han Sang YooDNA library was prepared according to the Illumina TruSeq DNA PCR-Free Library Prep protocol (Cat. 20015963). For sample library preparation, 2 μg of 550 bp inserts of high molecular weight genomic DNA was randomly

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TruSeq DNA PCR-Free High Throughput Library Prep Kit from Illumina

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TruSeq DNA PCR-Free High Throughput Library Prep Kit from Illumina

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