Nextera XT DNA Library Preparation Kit (96 Samples) FC-131-1096 from Illumina

Product Specs
ItemNextera XT DNA Library Preparation Kit (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog NumberFC-131-1096
ApplicationsAmplicon sequencing, WGS, De novo sequencing, Metagenomics
ReactivityAny
TypeDNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Children’s gut microbiota predicts the efficacy of obesity treatmentGut MicrobesDecember 31, 2026Mireia Alcázar, Verónica Luque, Natalia Ferré, Judit Muñoz-Hernando, Mariona Gispert-Llauradó, Ricardo Closa-Monasterolo, Albert Feliu, Gemma Castillejo, Joaquín Escribanowas used to initiate the protocol. The multiplexing step was performed with a Nextera XT Index Kit (FC-131-1096) (Illumina, San Diego, CA, USA). One μl of the PCR product was run on a Bioanalyzer DNA 1000 chip to

(2) Rapid clinical validation of an RNA/DNA hybrid tagmentation-based metagenomic workflow for respiratory RNA virus detectionFront MicrobiolJuly 15, 2026Xia Ma, Shan Guo, Yangyang Feng, Murong Su, Feili Wei, Xuejun Liuperformed using an optimized protocol adapted from the Nextera XT DNA Library Prep Kit (Illumina, Cat. No. FC-131-1096). Compared with the previously described CATCH workflow, optimization focused on consolidating enzymatic

(3) The maternal KRAB-ZFP ZFPOBI1 reveals structural constraints governing ERV transcriptional co-option in mouse oocytesFront Cell Dev BiolJuly 14, 2026Andrina J. Stäubli, Yan Huang, Louise J. H. Rasmussen, Freja Mosegaard Hansen, Hui Mun Loh, Michael J. Claxton, Daniel M. Messerschmidtperformed on 1 ng of cDNA per reaction using the Illumina Nextera XT DNA sample preparation kit (Illumina, FC-131-1096). NGS libraries were amplified for 10 cycles, pooled and sequenced Illumina NextSeq2000 using 100 bp

(4) Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivoJ Biol ChemJuly 6, 2026Niranjana Natarajan, Thaybah Ahmed, Balaji Govindaswamy, Devika S. Manickam, Jishnu Das, Kabirul Islam, Partha Duttawith full length cDNA were advanced to library preparation using Illumina Nextera XT kit (Illumina: FC-131-1096) with 12 PCR cycles. Libraries were pooled and sequenced on an Illumina NextSeq 500 using a High Output

(5) Isolation and Characterization of ΦCA1NRNZ, a Lytic Bacteriophage Targeting the Emerging Device-Associated Pathogen Cutibacterium avidumAntibiotics (Basel)July 3, 2026Ron Braunstein, Amit Rimon, Roni Teitelbaum, Suhnit Coppenhagen-Glazer, Vered Molho-Pessach, Ronen Hazansequencing. Sequencing libraries were constructed using the Nextera XT DNA Library Preparation kit (FC-131-1096, Illumina Inc., San Diego, CA, USA) according to the manufacturer’s protocol, with reagent and input

(6) Single-bacterium RNA-seq protocol to uncover heterogeneous expression of coding and noncoding genes in Bacteroides thetaiotaomicronSTAR ProtocJuly 1, 2026Elise Bornet, Alexander J. Westermannstore,KK2602/SKU:7958935001 MEGAshortscript kit,ThermoFischer Scientific,AM1354 Monarch RNA cleanup kit,NEB,T2050S Nextera XT kit,Illumina,FC-131-1096 Qubit dsDNA HS assay kit,Invitrogen,Q32854 TE buffer,Invitrogen,AM9849 Oligo Clean & Concentrator,Zymo Research

(7) Multimodal characterization of variation in neuronal types in the mouse basal gangliabioRxivJune 29, 2026Agata Budzillo, Matt Mallory, Rachel Dalley, Changkyu Lee, Xuehan Ci, Xiao-Ping Liu, Sarah Walling-Bell, Rusty Mann, Nelson Johansen, Ellie Adams, et al.in the SMART-Seq v4 kit. All samples proceeded through Nextera XT DNA Library Preparation (Illumina FC-131-1096) using either Nextera XT Index Kit V2 Set A-D (FC-131-2001,2002,2003,2004) or custom dual-indexes provided

(8) Type I Interferon-Driven Monocyte Dysregulation and MAS-associated CD8 + T cells During Macrophage Activation SyndromebioRxivJune 25, 2026Susan P. Canny, Hannah A. DeBerg, Emma L. Kuan, Nicholas Moss, Griffin Gessay, Ailing Lu, Amanda Huang, Allison R. O’Rourke, Erik D. Layton, Paige Bouvatte, et al.for Sequencing (Takara Bio USA, Cat #634891) and Nextera XT DNA Library Preparation Kit (Illumina, FC-131–1096). Libraries were pooled, quantified by a Qubit Fluorometer (Life Technologies) and sequenced on a NextSeq

(9) Monocytic niches escape T cell surveillance and promote Mycobacterium tuberculosis persistence in lymph nodesImmunityJune 23, 2026Elya A. Shamskhou, Fergal J. Duffy, Lauren M. Cross, Courtney R. Plumlee, Benjamin H. Gern, Holly W. Barrett, Alan H. Diercks, Sara B. Cohen, Ramya Sivakumar, John D. Aitchison, et al.v4 Ultra Low Input RNA Kit,Takara Bio,Cat# 88-8824-00 Nextera XT DNA Library Prep Kit,Illumina,Cat# FC-131-1096 LymphoLyte,Cedarlane,Cat# CL5035 Deposited data,Deposited data,Deposited data Mycobacterium tuberculosis

(10) Longitudinal changes in DNA methylation in IDH-mutant glioma fuel disease progression through altered cell state differentiationNature GeneticsJune 22, 2026Masashi Nomura, Ramya Raviram, Joshua S. Schiffman, Lillian Bussema, Vivian Lu, Noelle Wheeler, John J. Y. Lee, Yilin Fan, Mian Hua Zheng, Florian Ruiz, et al.(Beckman, A63882) purification. Libraries were tagmented using the Nextera XT Library Prep kit (Illumina, FC-131-1096) with custom barcode adaptors. Libraries from 768 cells with unique barcodes were pooled and sequenced

(11) Multiple Reassortments, Limited Geographic Spread, and Comparable Pathogenicity of Crimean-Congo Hemorrhagic Fever Viruses in Sivas Province, TürkiyeJ Med VirolJune 22, 2026Qianying Lin, Nazif Elaldi, Mesut Yigit, Baris Yildiz, Roger Hewson, Martín López-García, Grant Lythe, Ayse Nur Pektas, Binnur Koksal, Tuba Nur Tasseten, et al.Diagnostics, Türkiye). Illumina sequencing libraries were prepared using the Nextera XT v2 kit (Cat. No. FC‐131‐1096, Illumina) and sequenced on a 2 × 150 bp paired‐end Illumina MiSeq platform operated by Gen‐Era Diagnostics.

(12) Protocol for simultaneous profiling of transcription start sites and full-length transcripts from low-input samples using Smart-seq+5′STAR ProtocolsJune 19, 2026Diego Rodriguez-Terrones, Marlies E. Oomen, Maria-Elena Torres-Padillacommercial assays ,, Kapa HiFi ReadyMix,Roche,KM2605 Nextera XT DNA Library preparation kit,Illumina,FC-131-1096 Qubit dsDNA HS Assay Kit,ThermoFisher Scientific,Q32854 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, dT30V

(13) Skin-derived G-CSF activates pathological granulopoiesis upon psoriasisEMBO Molecular MedicineJune 16, 2026Tomson Kosasih, Tatsuya Morishima, Sohyeon Lee, Jungyeon Yoon, Kanako Wakahashi, Pilhan Kim, Aiko Sada, Hitoshi TakizawaM0212L dNTPs,Takara,Cat# 4030 AMPure XP beads,Beckman Coulter,Cat# A63881 Tagment DNA buffer,Illumina,Cat# FC-131-1096 Index primers,Illumina,Cat# FC-131-2001 High Sensitivity D5000 Screen Tape,Agilent,Cat# 5067-5592 GenNext®

(14) Coronavirus protein interaction mapping in bat and human cells reveals network rewiring governing immune evasion and zoonotic potentialCell Host & MicrobeJune 10, 2026Jyoti Batra, Magdalena Rutkowska, Yuan Zhou, Chengjin Ye, Rithika Adavikolanu, Janet M. Young, Durga Anand, Sooraj Verma, Haripriya Parthasarathy, Martin Gordon, et al.(Beckman Coulter, A63882) and used as input for library preparation using the Nextera XT kit (Illumina, cat. FC-131–1096) and sequenced in paired-end mode (2×150 nt) on the Illumina MiSeq. Genome assembly and quality control

(15) Whole genome sequence of Tsukamurella tyrosinosolvens extracted from metagenome of human pleural fluid enriched in Mycobacteria Growth Indicator TubeMicrobiology Resource AnnouncementsJune 9, 2026Rameez Moidu Jameela, Mayur Mahindra Kedare, Rehbar Khan, Nehal Dhankad, Rishav Kumar Sinha, Amrutraj Zade, Sanchi Shah, Anirvan Chatterjeeprepared from this extracted DNA using the Nextera XT DNA Library Preparation Kit (Illumina Cat. No. FC-131-1096) and sequenced using DNBSEQ-T7 machine from MGI as per manufacturer’s protocol. Quality of such generated

(16) Optimisation and validation of capture mNGS for predicting antimicrobial resistanceEBioMedicineJune 3, 2026Qiao Lin, Xu Mei, Huimin Zheng, Jie Meng, Fusheng He, Bin Yang, Xiao Ru, Mengting Su, Danlan Wang, Ni Tan, et al.(Qiagen, 50214). DNA libraries were constructed using the Nextera XT DNA Library Prep Kit (Illumina, FC-131-1096) according to the manufacturer's protocol. DNA libraries underwent probe hybridisation and enrichment

(17) Integration of Transcriptional Signatures from Brain Tissue and Plasma Extracellular Vesicles of a Preclinical Tauopathy Mouse ModelInt J Mol SciJune 3, 2026Tanzima Tarannum Lucy, A. N. M. Mamun-Or-Rashid, Daniel C. Lee, Iliya Lefterov, Radosveta Koldamova, Nicholas Francis FitzKit (#634773, Takara Bio, San Jose, CA) combined with the Nextera XT DNA Library Preparation Kit (#FC-131-1096, Illumina Inc., San Diego, CA, USA) and Illumina DNA/RNA UD Indexes Set A (#20091654, Illumina, CA).

(18) Pre-treatment bacterial cell states shape antibiotic-induced transcriptional reprogramming and survivalNature CommunicationsJune 2, 2026Qiang Liu, Zehui Yu, Xiaoli Liu, Amy Iverson, Tyler Simmons, Jason W. Rosch, Peijun Masequencing Sequencing libraries were prepared using the Illumina Nextera XT DNA Library Prep Kit (FC-131-1096, Illumina) with protocol adjustments. For each 20 μl tagmentation reaction, 1 to 2 ng of enriched cDNA

(19) Validation of Brachial Vein Endothelial Transcriptomics to Assess the Coronary VasculatureCirc ResJune 1, 2026Michael S. Garshick, Florencia Schlamp, Isabelle Boothman, Tessa Barret, Filipp Kazatsker, Gael Westby, Yuhe Xia, Nathaniel R. Smilowitz, Sanja Jelic, Naomi Hamburg, et al.Micro Kit,Qiagen,74004 SMART-Seq mRNA HT,Takara Bio,634791 Nextera XT DNA Library Prep Kit,Illumina,FC-131–1096 Major Resources Tables

(20) Transient tumor exposure induces persistent functional defects in memory CD8+ T cellsiScienceMay 15, 2026Daphné Laubreton, Margaux Prieux, Sophia Djebali, Maxence Dubois, Simon De Bernard, Olivier Gandrillon, Christophe Arpin, Jacqueline MarvelBiosystem,Cat#KK2602 Agencourt AMPure XP,Beckman,Cat# A63880 Nextera XT DNA sample preparation kit,Illumina,Cat#FC-131-1096 Nextera XT 96-index kit,Illumina,Cat#FC-131-1002 ,, Critical commercial assays,Critical commercial

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Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina

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Nextera XT DNA Library Preparation Kit (96 Samples) from Illumina

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