Illumina Stranded mRNA Prep, Ligation (16 Samples) 20040532 from Illumina

Product Specs
ItemIllumina Stranded mRNA Prep, Ligation (16 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20040532
ApplicationsmRNA-Seq, Stranded RNA-Seq
ReactivityHuman, Mouse, Rat, Bovine
TypeRNA Library Prep Kit
Quantity16 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Paired mutation calling and spatial transcriptomics identify cellular neighborhoods associated with the neoplastic outcome of mouse colitisNat GenetJuly 28, 2026Elisa B. Moutin, Linus L. H. Chang, Giada Giavara, Shenay Mehmed, Mathilde Colombé, Carla Boquetale, Kate Marks, Filipe C. Lourenço, Nefeli Skoufou-Papoutsaki, Richard Kemp, et al.performed by the CRUK Cambridge Institute Genomics Core using the Illumina Stranded mRNA Prep kit (20040532, Illumina) according to manufacturer’s instructions. Samples were sequenced using the Illumina Novaseq

(2) Essential lncRNAs in the human transcriptomeCell GenomicsJuly 8, 2026Wen-Wei Liang, Simon J. Mueller, Sydney K. Hart, Hans-Hermann Wessels, Alejandro Méndez-Mancilla, Akash Sookdeo, Olivia Choi, Christina M. Caragine, Alba Corman, Lu Lu, et al.using the Direct-zol RNA Purification Kit (Zymo R2062). We used the Stranded mRNA Prep kit (Illumina 20040532) with RNA UD Indexes Set A (Illumina 20040553) and followed the manufacturer’s protocol using 1 μg total

(3) Simultaneous Transcriptomic Analysis of Both Host and Symbiont in Insect–Fungus InteractionsBio ProtocJuly 5, 2026McKeon Laws, Ellie S. Burns, Matt T. Kason, Teiya Kijimoto, Jason E. Stajichtubes (multiple manufacturers) 9. Illumina Stranded mRNA Prep, Ligation Kit (Illumina, catalog number: 20040532) 10. AMPure XP beads for DNA cleanup (Beckman Coulter, product number: A63880) Note: These are required

(4) Cerebellar microRNA-206 tunes Purkinje neuron firing dynamics to control sensorimotor gatingbioRxivJune 30, 2026Mary P. Heyer, Masago Ishikawa, Junshi Wang, John Erol Evangelista, Amanda K. Fakira, Avi Ma'ayan, Guoping Feng, Paul J. KennyHigh Sensitivity DNA kit,Agilent,Cat#5067-4626 Illumina Stranded mRNA library prep kit,Illumina,Cat#20040532 NovaSeq 6000 S1 Reagent Kit v1.5 (300 cycles),Illumina,Cat#20028312 NextSeq 500 High Output v2.5 kit

(5) Genetic inactivation of Translin/Trax RNase disrupts miRNAs, hippocampal synaptic plasticity, and memoryiScienceJune 19, 2026Mahesh Shivarama Shetty, Junko Kasuya, Xiuping Fu, Marisol Carmela Lauffer, Satya Murthy Tadinada, Tania Chatterjee Chowdhury, Zeru Peterson, Knute D. Carter, Jay M. Baraban, Ted AbelRNA Library Preparation Kit,Illumina,Cat # RS-200 Illumina Stranded mRNA Library Preparation Kit,Illumina,Cat # 20040532 iScript cDNA Synthesis Kit,Bio-Rad,Cat # 1708890 SYBR™ Green Universal Master Mix,Thermo Fisher,Cat

(6) Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cellsNature CommunicationsMay 20, 2026Pamuditha N. Silva, Johanna E. Mayrhofer, Pavlo Potalitsyn, Geraldine O. Trüllinger, Svenja Godbersen, Nic Amstutz, Sebastian M. Siegner, Ben Kodiyan, Alexandra C. Title, Burcak Yesildag, et al.oligo-d(T)-based poly(A)+ RNA capture with the Illumina Stranded mRNA Prep, Ligation kit (Illumina 20040532). Guanidine/inosine (G/I) tailing of the poly(A) RNA was performed using reagents from the Poly(A) Tail-Length

(7) Targeting Host Metabolic and Epigenetic Rewiring Blocks Lytic Gammaherpesvirus ProductionVirusesMay 19, 2026Morgan C. Jones, Tina M. Le, Connor J. Mahoney, Sara K. Hartman, Robynne D. Dona, Yennifer A. Gaspar, Sennah J. Hong, Benjamin R. Sheirbon, Thelma M. Escobar, Tracie Delgadototal RNA was used for library preparation using the Illumina Stranded mRNA Prep kit (Illumina No. 20040532, San Diego, CA, USA), which includes poly(A) selection. Libraries were indexed using the Illumina DNA/RNA

(8) Modeling human enterovirus A71 infection using an intestinal microphysiological systemJournal of VirologyMay 19, 2026Hiroki Futatsusako, Sayaka Deguchi, Kaori Kosugi, Rina Hashimoto, Noriyo Nagata, Tadaki Suzuki, Takuya Yamamoto, Kazuo TakayamaRNA-seq libraries were constructed using an Illumina Stranded mRNA Prep, Ligation Kit (Illumina, Cat#20040532) according to the manufacturer’s instructions. Sequencing was performed on an Illumina NextSeq 2000

(9) Protein Synthesis Profiling Enables In Vivo Capture of Ribosome-Associated mRNAs in YeastbioRxivMay 5, 2026Cesar Arcasi Matta, Zhi Qi Ten, Simpson Josephbiotinylated RNA was used for library preparation with the Illumina Stranded mRNA Prep Ligation Kit (Cat# 20040532), following the manufacturer’s instructions. Four biological replicates per condition were processed

(10) γδ T cell-stromal networks modulate matrix composition and vascularity in foreign body responseNature CommunicationsApril 17, 2026Anna Ruta, Kavita Krishnan, JiWon Woo, Joscelyn C. Mejías, Elise F. Gray-Gaillard, David R. Maestas Jr., Helen Hieu Nguyen, Alexandra N. Rindone, Christopher Cherry, Michael Patatanian, et al.preparation with unique dual indexes (UDI) was performed using Stranded mRNA Prep, Ligation kit (Illumina 20040532). QC was assessed with Qubit and Agilent Fragment Analyzer. Libraries were pooled and pair-end sequencing

(11) Structural variants in human congenital heart disease disrupt distal genomic regulatory contacts of developmental genesbioRxivMarch 2, 2026Jodi Lee, Jingshing Wu, Maureen Pittman, Zoe L. Grant, Shuzhen Kuang, Daniel Quiat, Sarah U. Morton, Geoff Fudenberg, Michela Traglia, Kelly A. Hayes, et al.manufacturer’s instructions. RNA samples were processed using the Illumina Stranded mRNA Prep Ligation Kit (20040532) according to manufacturer’s instructions. For each sample, 250ng of total RNA (quantified on the Invitrogen

(12) Synergy between regulatory elements can render cohesin dispensable for distal enhancer functionScienceFebruary 5, 2026Karissa L. Hansen, Annie S. Adachi, Luca Braccioli, Smit Kadvani, Ryan M. Boileau, Moreno Martinovic, Bozhena Pokorny, Rini Shah, Erika C. Anderson, Kaite Zhang, et al.as input for poly(A) capture and library preparation using the Illumina Stranded mRNA kit (Illumina 20040532) per manufacturer’s instructions, and DNA quality was assessed using the Bioanalyzer High Sensitivity

(13) Stage-dependent dual mechanisms of MORC2 in retrotransposon silencing and sex chromosome inactivation in germ cellsNucleic Acids ResDecember 11, 2025Zhenlong Kang, Rui Guo, Shenglin Han, Fang Yang, Jinwen Zhang, Yuanyuan Wang, Kara A Nelson, Vamshidhar C Nallamala, N Adrian Leu, Zhengyi Li, et al.(Invitrogen). RNA-seq libraries were prepared using the Illumina Stranded mRNA Prep kit (Illumina, 20040532), pooled, and sequenced on the Illumina NovaSeq 6000 platform at the UPenn Next-Generation Sequencing

(14) Focal adhesion assembly and cell migration require myoferlin in PDAC cell linesScientific ReportsNovember 28, 2025Charlotte Gullo, Emilie Laverdeur, Manon Dancre, Raphaël Peiffer, Yasmine Boumahd, Ferman Agirman, Naïma Malouhjamoum, Laury Désiront, Marc Thiry, Gilles Rademaker, et al.(5067-1511, Agilent). Next, a mRNA library was generated using the Illumina stranded mRNA Prep Ligation kit (20040532, Illumina) according to manufacturer’s instructions and sequencing was performed on an Illumina Novaseq6000

(15) Comprehensive androgen-dependent transcriptome analysis in human genital tissueBMC GenomicsNovember 17, 2025Radhika Sivaprasad, Kristian Händler, Almuth Caliebe, Malte Spielmann, Paul-Martin Holterhus, Nadine C. Hornigconverted into sequencing libraries using the Illumina Stranded mRNA Prep Ligation kit (Illumina, Cat. No. 20040532, San Diego, USA). Sample-specific barcoding was achieved utilizing Illumina Unique Dual (UD) Set A index

(16) Fecal exfoliome sequencing captures immune dynamics of the healthy and inflamed gutNature BiotechnologyNovember 17, 2025Yiming Huang, Yiwei Sun, Carlotta Ronda, Chrystal F. Mavros, Jing Li, Justin Jacobse, Lei H. Huang, Samuel J. Resnick, Marla Giddins, Daniel E. Freedberg, et al.library were prepared for RNA from mouse colon tissue using Illumina Stranded mRNA Prep kit [Illumina 20040532] according to manufacturer’s instructions. Yielding RNA-seq libraries that were sequenced on Illumina

(17) DICER1 hotspot mutation induces 3p microRNA gain of function via Argonaute strand switchNature Structural & Molecular BiologyNovember 4, 2025Sharan Malagobadan, Chunmei Shi, Acong Yang, Indranil Mondal, Habikah Baldeh, Karrie Spain, Wilfried Guiblet, Shuo Gurepression. RNA-seq The library for mRNA-seq was generated using Illumina Stranded mRNA Prep (Illumina, 20040532) and sequenced on a NovaSeq 6000 SP using paired-end sequencing by the NCI CCR Sequencing Facility. CLASH CLASH

(18) The Human Omental Adipose Depot Mitigates Inflammation, Immune Response, and Oxidative Stress Pathways in Response to Injury via Its SecretomeBiology (Basel)October 28, 2025Meredith Krause-Hauch, Rekha S. Patel, Bangmei Wang, Brianna Jones, Paul Albear, Niketa A. PatelLibrary Prep Kit was used according to the manufacturer’s instructions (Illumina, San Diego, CA, USA, Cat#: 20040532). The DNA libraries were checked for quality and concentration prior to loading samples into the Illumina

(19) Prediction of the hypothalamus-pituitary organoid formation using machine learningCell Reports MethodsAugust 18, 2025Ryusaku Matsumoto, Hidetaka Suga, Yutaka Takahashi, Takashi Aoi, Takuya Yamamotoanalysis. RNA-seq library construction was performed using an Illumina Stranded mRNA Prep Kit (Cat# 20040532, Illumina) according to the manufacturer’s instructions. Obtained libraries were sequenced with NextSeq

(20) METTL9 sustains vertebrate neural development primarily via non-catalytic functionsNature communicationsAugust 1, 2025Codino A, Spagnoletti L, Olobardi C, Cuomo A, Santos-Rosa H, Palomba M, Margaroli N, Girotto S, Scarpelli R, Luan SL, et al.experiments (NF22), transcriptomic libraries were generated using the Stranded mRNA Prep Kit (Illumina, #20040532) starting from 200 ng of total RNA. Equimolar amounts of libraries were loaded and sequenced on a NovaSeq

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Illumina Stranded mRNA Prep, Ligation (16 Samples) from Illumina

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Illumina Stranded mRNA Prep, Ligation (16 Samples) from Illumina

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