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Assay Kits
Assay Kit
TruSeq RNA Library Prep Kit v2 Set B (48 Samples)
TruSeq RNA Library Prep Kit v2 Set B (48 Samples) from Illumina
Be the first to write a review!
Citations:
(211)
Supplier Page
Supplier Page from
Illumina for
TruSeq RNA Library Prep Kit v2 Set B (48 Samples)
Description
Simple, cost-effective non-stranded mRNA library prep kit for analysis of the coding transcriptome with minimal hands-on time; Set B indexes
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TruSeq RNA Library Prep Kit v2 Set B (48 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
RS-122-2002
Applications
mRNA-Seq
Reactivity
Human, Mouse, Rat, Bovine
Type
RNA Library Prep Kit
Quantity
48 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (211)
(1)
5-Aminolevulinic Acid Drives Coordinated Astaxanthin and Lipid Accumulation in Green Alga Chromochloris zofingiensis
Foods
May 17, 2026
Jinrui Gao, Zhongliang Sun, Bin Liu, Yu Zhang, Liqin Sun
instructions. RNA
library construction
was performed using the TruSeq RNA Sample Preparation Kit (Cat. No.
RS-122-2002
,
Illumina
, San Diego, CA, USA) according to the manufacturer’s instructions.
Sequencing
was conducted
(2)
Increased leptin signaling drives the response of hypothalamic LepRb neurons to diet-induced obesity
Mol Metab
May 2, 2026
James Dell'Orco, Warren Pan, Margaret B. Allison, Abigail J. Tomlinson, Jordan Wean, Paul V. Sabatini, Christopher J. Rhodes, David P. Olson, Martin G. Myers Jr., Paulette B. Goforth
greater were prepared using the Illumina TruSeq mRNA Sample Prep v2 kit (Catalog #
RS-122-2001
and #
RS-122-2002
) (
Illumina
, San Diego, CA), where 0.1–3 μg of RNA was converted to mRNA using a polyA
purification
.
(3)
Persistent PI3K–AKT signaling fortifies cellular defense against oxidative stress and ferroptosis through augmented mitochondrial fitness
Cellular & Molecular Biology Letters
March 14, 2026
Xin Xie, Shanshan Wang, Wentao Zeng, Xiaohang Long, Daheng Zheng, Jianping Ye, Rachid Rezgui, Pu-Ste Liu
(
Qiagen
,
74106
).
RNA-sequencing
libraries were prepared using TruSeq RNA Library Prep Kit v2 (
Illumina
,
RS-122-2002
) based on the manufacturer’s instructions. Library quality and size were analyzed using Bioanalyzer
(4)
Biomimetic Copper‐Doped Nano‐Aluminum Adjuvant Potentiates Therapy in Chemoresistant Acute Myeloid Leukemia
Advanced Healthcare Materials
March 10, 2026
Chao He, Lingxiao Zhang, Yue Xiong, Yu Wang, Yuqing Zeng, Dandan Su, Ran Wang, Ying Li, Hongbo Chen, Fang Cheng,
et al.
DNA (cDNA), following the TruSeq RNA Sample Preparation v2 protocol (catalog numbers
RS‐122‐2001
and
RS‐122‐2002
,
Illumina
, USA). The resulting paired‐end libraries had an average insert size of 300 ± 50 base pairs
(5)
NSD2 upregulation is driven by high-risk HPV E6/E7 and disrupts epithelial differentiation in HPV-associated head and neck cancer
Journal of Experimental & Clinical Cancer Research
January 8, 2026
Lavinia Ghiani, Simona Citro, Alessandro Medda, Mirko Doni, Farkhondeh Ghoryani, Roberta Noberini, Ottavio Croci, Fausto Maffini, Claudia Miccolo, Laura Monteleone,
et al.
used to prepare library for
RNA sequencing
using the Truseq RNA Sample Prep Kit V2set B (
Illumina
#
RS-122–2002
) according to the manufacturer instructions.
Sequencing
was performed using
NovaSeq 6000 system
(
Illumina
)
(6)
Substrain-Dependent Differences in Doxorubicin-Induced Cardiotoxicity in Adult C57BL/6 Mice
Cardiovasc Toxicol
December 23, 2025
Mary R. Daniel, Marianne K. O. Grant, Mohamed S. Dabour, Maria Razzoli, Fernando Souza-Neto, Jop H. van Berlo, Alessandro Bartolomucci, Beshay N. Zordoky
used for library preparation using either the TruSeq RNA Sample Preparation Kit (Cat. #
RS-122–2001
or
RS-122–2002
) or the TruSeq Stranded mRNA
Library Prep
Kit (Cat. #RS-122–2101;
Illumina
, San Diego, CA, USA), following
(7)
Fosl1 is a transcriptional effector of BRAF V600E-driven intestinal tumorigenesis
iScience
November 21, 2025
Zakia Alam, Rebecca Nightingale, Analia Lesmana, Cheng Liu, Laura J. Jenkins, Mark F. Richardson, Lawrence Croft, Ian Y. Luk, Camilla M. Reehorst, Fiona Chionh,
et al.
LT07-318
GenePrint® 10 System
,
Promega
,Cat#
B9510
TruSeq
RNA Library Preparation
Kit v2,
Illumina
,"Cat#
RS-122-2001
,
RS-122-2002
" ,, Deposited data,Deposited data,Deposited data ,,
RNA-seq
data,This paper,GEO: GSE306906 ,,
Experimental models
(8)
A histone-centric multi-omics study shows that increased H3K4 methylation sustains triple-negative breast cancer phenotypes
Nature Communications
September 30, 2025
Roberta Noberini, Giulia Robusti, Alessandro Vai, Evelyn Oliva Savoia, Maria Giovanna Jodice, Giovanni Bertalot, Betül Çat, Isabella Pallavicini, Giuseppina Bonizzi, Maria Capra,
et al.
Table 2. Libraries for
RNA sequencing
were prepared with the TruSeq RNA Sample Preparation v2 kit (
RS-122-2002
,
Illumina
), starting from 100 ng of total RNA for clinical samples.
Sequencing
was performed using a
(9)
Identification of GREM-1 and GAS6 as Specific Biomarkers for Cancer-Associated Fibroblasts Derived from Patients with Non-Small-Cell Lung Cancer
Cancers (Basel)
August 30, 2025
Bo-Guen Kim, Kyunghee Park, Mina Hwang, Hyewon Lee, Kyung-Mi Park, Junsu Choe, Sun Hye Shin, Byeong-Ho Jeong, Kyungjong Lee, Junghee Lee,
et al.
RNA Sample Preparation Kit v2, set A and B (
Illumina, Inc
., San Diego, CA, USA, Cat#
RS-122-2001
and
RS-122-2002
) following manufacturer’s protocols. Paired-end
sequencing
(2 × 100 bp) of the RNA libraries was performed
(10)
LIN-39 is a neuron-specific developmental determinant of longevity in Caenorhabditis elegans with reduced insulin signaling
Nat Commun
July 16, 2025
Alan Kavšek, Jérôme Salignon, Lluís Millan-Ariño, Patryk Marcinkowski, Ilke Sen, Christian G. Riedel
then constructed, using the TruSeq
RNA Library Preparation
Kit v2 (
Illumina
, Cat. No.:
RS-122-2001
/
RS-122-2002
) according to the manufacturer’s instructions. Libraries were eventually submitted for 50 bp single-end
(11)
Retained introns in phototransduction genes of 5xFAD mouse retina suggest vision impairment as an early diagnostic marker for Alzheimer's disease
Sci Rep
July 15, 2025
Hyun-Min Lee, Jinho Kim, Ji-Young Kim, Mi-Jin An, Geun-Seup Shin, Ah-Ra Jo, Yuna Park, Chul-Hong Kim, Yujeong Hwangbo, Ju-Hyun Lee,
et al.
was prepared using the TruSeq RNA Sample Preparation Kit v2 (
Illumina, Inc
., San Diego, CA, cat. no.
RS-122–2002
). Briefly, 100 ng of total RNA from each sample was utilized. Poly-A mRNA was isolated by binding to
(12)
Transcriptomic analysis reveals shared deregulated neutrophil responses in COVID-19 and idiopathic pulmonary fibrosis
Respiratory research
June 11, 2025
Divolis G, Synolaki E, Tringidou R, Tzouvelekis A, Boumpas DT, Skendros P, Galani IE.
preparation, 1 μg of total RNA was processed using the TruSeq
RNA Library Preparation
Kit v2 (
Illumina
,
RS-122-2002
), as previously described [32–33].
Sequencing
was carried out at the Greek Genome Center (BRFAA, Athens,
(13)
Transcriptome profile of early matured Macrobrachium rosenbergii (Giant Freshwater Prawn) from Taiwan
Scientific Data
June 3, 2025
Sofia Priyadarsani Das, Yu-Ru Lin, Yu-Sheng Wu, Chun-Hung Liu, Yeh-Fang Hu, Fan-Hua Nan
Library preparation The TrueSeq RNA Sample Preparation Kit v2 (
Illumina
, Cat. No.
RS-122-2001
and/or
RS-122-2002
) was used to create an
mRNA–Seq
library in accordance with the manufacturer’s instructions. The Illumina
(14)
Investigating oncoprotein-mediated chromatin dysregulation in Drosophila melanogaster uncovers novel modifiers of the developmental impact of H3 K27M and EZHIP
bioRxiv
May 28, 2025
Samuel D. Krabbenhoft, Tyler E. Masuda, Yadwinder Kaur, Truman J. Do, Siddhant U. Jain, Peter W. Lewis, Melissa M. Harrison
RNA isolation
procedure
, and libraries were prepared using the TruSeq RNA sample prep kit v2 (Ilumina
RS-122-2002
). 75-base-pair reads were obtained using an
Illumina
NextSeq500 High-Throughput Sequencer.
Sequencing
(15)
Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia
PLoS Pathog
May 13, 2025
Qinyu Luo, Lihua Shen, Shiyue Yang, Yan Zhang, Yihang Pan, Zehua Wu, Qiang Shu, Qixing Chen
library in accordance with the protocol for TruSeq RNA Sample Preparation v.2 (
Illumina
,
RS-122–2001
and
RS-122–2002
); the average insert size for the paired-end libraries was 300 bp (±50 bp). Paired-end
sequencing
was
(16)
Mapping effective microRNA pairing beyond the seed using abasic modifications
Nucleic Acids Res
April 29, 2025
David M Kosek, Katja Petzold, Emma R Andersson
Sequencing
libraries were prepared with the TruSeq RNA Sample Preparation v2 kit (
Illumina
,
RS-122-2001
/
RS-122-2002
), according to the manufacturer’s protocol. The quality of the complementary DNA libraries was checked
(17)
An evolutionarily conserved constellation of functional cis-elements programs the virus-responsive fate of the human (epi)genome
Nucleic Acids Res
March 25, 2025
Marianna A Koutsi, Marialena Pouliou, Dimitris Chatzopoulos, Lydia Champezou, Konstantinos Zagkas, Marili Vasilogianni, Alexandra G Kouroukli, Marios Agelopoulos
RNA sequencing
libraries were generated using the TruSeq RNA Library Prep Kit v2, Set A and B (
RS-122-2001
and
RS-122-2002
) for
Illumina
following the manufacturer’s instructions [17] or the NEBNext Ultra II Directional RNA
(18)
Nutrient control of splice site selection contributes to methionine addiction of cancer
Molecular Metabolism
March 1, 2025
Da-Wei Lin, Francisco G. Carranza, Stacey Borrego, Linda Lauinger, Lucas D Dantas de Paula, Harika R. Pulipelli, Anna Andronicos, Klemens J. Hertel, Peter Kaiser
preparation was performed using the TruSeq
RNA Library Preparation
Kit v2 (
Illumina
,
RS-122-2001
and
RS-122-2002
) with the
ERCC RNA Spike-In mix
(
ThermoFisher
,
4456740
) to control for sample preparation variation.
(19)
KAT6B overexpression rescues embryonic lethality in homozygous null KAT6A mice restoring vitality and normal lifespan
Nat Commun
February 25, 2025
Maria. I. Bergamasco, Yuqing Yang, Alexandra L. Garnham, Bilal N. Sheikh, Gordon K. Smyth, Anne. K. Voss, Tim Thomas
RNA used to generate libraries using a
library construction
kit (TruSeq RNA prep kit v2;
Illumina
,
RS-122-2002
), according to the manufacturer’s instructions. Samples were run on a
sequencing
machine (NextSeq 2000;
(20)
Porcine myogenesis in cloned wildtype and MYF5/MYOD/MYF6-null porcine embryo
Commun Biol
February 11, 2025
Yong-Ho Choe, Satyabrata Das, Xiao Ma, Hyeonjeong Lee, Jacob R. Sorensen, Daniel B. Hoffman, Chan-Hee Jo, Casey P. Johnson, Nicolette Cassel, Daniel J. Garry,
et al.
Illumina sequencing
libraries using
Illumina
’s TruSeq RNA Sample Preparation Kit (Cat. #
RS-122-2001
or
RS-122-2002
) or Stranded mRNA Sample Preparation Kit (Cat. # RS-122-2101). (Please see www.illumina.com for a detailed
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TruSeq RNA Library Prep Kit v2 Set B (48 Samples) from Illumina
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