Illumina Stranded mRNA Prep, Ligation (96 Samples) 20040534 from Illumina

Product Specs
ItemIllumina Stranded mRNA Prep, Ligation (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20040534
ApplicationsmRNA-Seq, Stranded RNA-Seq
ReactivityHuman, Mouse, Rat, Bovine
TypeRNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Coordinated neural progenitor adaptations contribute to primate neocortical downscalingSci AdvSeptember 25, 2026Lidiia Tynianskaia, Cesar Mateo Bastidas-Betancourt, Esther Marie Grewe, Julia Marie Kniep, Neringa Liutikaite, Nesil Esiyok, İrfan Burak Göloğlu, Sabrina Heide, Nancy Rüger, Dimitri Lindenwald, et al.on the STAR Hamilton NGS automation platform using the Illumina Stranded mRNA Prep Kit (catalog no. 20040534) and the IDT for Illumina RNA UD Indexes Set A, Ligation with 96 Indexes (catalog no. 20091646), starting

(2) A geothermal amoeba sets a new upper temperature limit for eukaryotesCellSeptember 22, 2026H. Beryl Rappaport, Natalie A. Petek-Seoane, Tomáš Tyml, Jessica K. Niblo, Felix Mikus, Naomi E. Gilbert, Kurt LaButti, Godwin Ani, Ethan MacVicar, Rachel M. Shepherd, et al.Monarch Spin RNA Isolation Kit (Mini),New England Biolabs,Cat#T2110S Illumina Stranded mRNA Prep,Illumina,Cat#20040534 DNEasy PowerSoil Kit,Qiagen,Cat#47014 Deposited data,Deposited data,Deposited data Analysis code

(3) Exercise intensity modulates interorgan communication and is associated with cardiometabolic health outcomes in humansCell Reports MedicineSeptember 15, 2026Luke Olsen, Javier Botella, Douglas Barrows, Ethan Romero, Kaitlyn Baird, Mutsumi Katayama, Ece Kilic, Christopher Peralta, Nadège Zanou, Henry Sanford, et al.ng of total RNA was used to generate RNA-Seq libraries using Illumina stranded mRNA kit (Catalog #: 20040534), following the manufactures protocol. Libraries prepared with unique dual indexes were pooled at equal

(4) Innervated human cardiac muscle model reveals sympathetic drivers of KCNH2-associated arrhythmiasNat CommunSeptember 2, 2026Lennart Valentin Schneider, Michael Gani Setya, Guobin Bao, Daniel Härtter, Aditi Methi, Dennis Krüger, Marie Kristin Schreiber, Ole Jensen, Elisavet Kanari, Kea Aline Schmoll, et al.using the STAR Hamilton NGS automation platform, applying the Illumina Stranded mRNA Prep Kit (Cat. No. 20040534) and the IDT for Illumina RNA UD Indexes Set A, Ligation with 96 Indexes (Cat. No. 20091646), starting

(5) Isthmin-2 is a first-trimester predictor of preeclampsia and fetal growth restrictionNat MedSeptember 1, 2026Ruoyu Miao, Sungsam Gong, Ulla Sovio, Irving L. M. H. Aye, Giulia Avellino, Jacqueline Guettler, Stephanie N. Rogers, Emma Cook, Ana Toribio, Lina Bergman, et al.sample to generate barcoded sequencing libraries with the Illumina Stranded mRNA prep ligation kit (#20040534) and the Illumina index kit A (#20091655). The quality of the cDNA libraries was validated using the

(6) Paternal genetic background orchestrates fetal development through tissue-specific cis-regulatory divergenceiScienceAugust 21, 2026Muhammad Arsalan Iqbal, Eduard Murani, Frieder Hadlich, Henry Reyer, Michael Oster, Nares Trakooljul, Klaus Wimmers, Siriluck Ponsuksilikit with 11 PCR-cycles of amplification according to the manufacturer’s recommendation (Illumina, Cat#20040534, Germany). The libraries were quality checked for fragment length distribution on Agilent 2100 Bioanalyzer

(7) Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite developmentElifeAugust 12, 2026Pascale Pescher, Thibaut Douché, Quentin Giai Gianetto, Karen Druart, Julie Kovářová, Blaise Li, Thomas Cokelaer, K Shanmugha Rajan, Laura Piel, Céline Besse, et al.Library Preparation Kit,Illumina,20015963, Commercial assay or kit,Illumina Stranded mRNA Prep,Illumina,20040534, "Chemical compound, drug",YO-PRO,Invitrogen,Y3306, "Chemical compound, drug",Propidium iodide,Sigma-Aldrich,P4864, "Chemical

(8) Tumoral extracellular vesicles and particles reprogram interstitial macrophages in the lung to promote vascular permeability and metastasisNat CancerAugust 5, 2026Shani Dror, Inbal Wortzel, Serena Lucotti, Yura Seo, Jianlong Li, Lee Shaashua, Irina Matei, Nancy Boudreau, Haiying Zhang, Maider Astorkia-Amiama, et al.performed by the Weill Cornell Medicine Genomics Core using the Illumina Stranded mRNA Library Prep kit (PN 20040534) on an Agilent Bravo system. Libraries were sequenced on an Illumina NextSeq 2000 (paired-end reads,

(9) PBRM1-dependent PBAF targeting is required for EMT and metastasis in breast cancerSci AdvJuly 31, 2026Alisha Dhiman, Mitchell G. Ayers, Guanming Jiao, Jamie L. McCuiston, Aparna B. Shinde, Saeed Salehin Akhand, Juan Jauregui-Lozano, Marco Hadisurya, Elizabeth G. Porter, W. Andy Tao, et al.isolation. Library construction was done using the Illumina Stranded mRNA prep, ligation kit (Illumina, #20040534) according to the manufacturer’s instructions and checked for quality using Qubit and Agilent Bioanalyzer

(10) Transcriptomic changes in the jerboa supraoptic nucleus during osmotic stress: PCSK1 and PTPRN changes are conserved across desert and mesic speciesJ NeuroendocrinolJuly 26, 2026Benjamin T. Gillard, Nabil Amor, Michael P. Greenwood, Abdulaziz N. Alagaili, David Murphyng of input total RNA was used in the Illumina Stranded mRNA library preparation with ligation kit (20040534) and followed with a 4 min fragmentation time. IDT for Illumina RNA UD Indexes (20,040,553, Set A) were

(11) Differential activation of NF-Κβ and HIF-1α between fetal liver-derived and myeloid-derived macrophages drives inflammatory differences following Mycobacterium abscessus infectionInfection and ImmunityJuly 14, 2026Haleigh N. Gilliland, Soledad Soverina, Kayla N. Conner, Taryn E. Vielma, Andrew J. Olive(Tecan). RNA sequencing and analysis The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the

(12) Effects of Red Onion Peel Extracts on Oxidative Stress in Caenorhabditis elegansBiomoleculesJuly 13, 2026Héctor Palacios, Miguel Ángel Rodríguez, Nerea Abasolo, Adrià Ceretó, Sara Martinez de Cripan, Camille Malterre, Kevin Leonard, Helena Torrell, Antoni Del Pino, Núria Canela, et al.Clara, CA, USA). Sequencing libraries were prepared using the Illumina Stranded mRNA Prep Kit (Cat. No. 20040534), following the manufacturer’s protocol. Paired-end sequencing (2 × 76 bp) was performed on the Illumina

(13) Tumor-derived complement C3 overexpression in STK11-mutant lung adenocarcinoma drives tumor growth and immune checkpoint inhibitor resistanceCancer Immunol ResJuly 2, 2026Sora Suzuki, Bojidar Kandar, Catrina Ting, Peter Deraska, Te-An Chen, Thejaswini Giridharan, Anm Nazmul H Khan, Kristopher Attwood, Han Yu, Kayla Catalfamo, et al.740955). Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit (Illumina 20040534). All sequencing libraries were prepared from 100ng total RNA. Library quality and quantity were verified,

(14) Targeting metabolic dependencies to reverse chemoradiotherapy resistance in colorectal cancerJ Exp Clin Cancer ResJune 23, 2026Maximilian Hellkamp, Simon Gertken, Jonas Buchloh, Julius-Leonard Hellwig, Lukas Ben Kowitzke, Ningjun Duan, Ilaria Gaspardo, Stefan Küffer, Michael Linnebacher, Philipp Ströbel, et al.for RNA-seq was performed in the STAR Hamilton NGS automation using the Illumina Stranded mRNA Prep (#20040534; Illumina) and the ID for Illumina RNA UD Indexes Set A, ligation with 96 indexes (#20091646; Illumina)

(15) Newly identified invasion drivers define the tumor front in oral squamous cell carcinomaCell Death DiscoveryJune 20, 2026Guillermo Flores, Slyn Uaroon, Juan A. R. Garay, Marion Vanneste, Jesse D. Riordan, Adam J. Dupuy, Kristina W. Thiel, Kaitriana E. Colling, Christopher S. Stipp, Li-Chun Lin, et al.used to prepare sequencing libraries using the Illumina stranded mRNA library preparation kit (Cat. #20040534, Illumina, Inc., San Diego, CA). The molar concentrations of the resulting indexed libraries were measured

(16) Patient-matched transcriptomics of in vivo and in vitro colonic epithelium substantiate organoids as a translational model for ulcerative colitisScientific ReportsJune 14, 2026Gunnar Andreas Enerhaug Walaas, Arun Sridhar, Lusie Frostvoll Kuraas, Siri Sæterstad, Amanda Yin Schioldborg, Atle Van Beelen Granlund, Arne Kristian Sandvik, Ann Elisabet Østvik, Ingunn Bakke, Torunn BrulandStates). RNA sequencing libraries were prepared using the Illumina Stranded mRNA Prep (Ligation) Kit (#20040534, Illumina), following the manufacturer’s protocol. Briefly, 300 ng total RNA was used as the starting

(17) A functional betalain-producing dioxygenase in Neolecta irregularis reveals an expanded evolutionary origin of the pathwayIMA FungusJune 5, 2026Pedro Martínez-Rodríguez, M. Alejandra Guerrero-Rubio, Paula Henarejos-Escudero, Samanta Hernández-García, Fernando Gandía-HerrerocDNA libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit (Illumina, catalog no 20040534), following the manufacturer’s instructions. The kit performs a PolyA capture followed by a ligation-based

(18) Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement via Single-Cell GenomicsbioRxivJune 1, 2026Federica Sozza, Alberto Romano, Nicole D'Elia, Martina Terenzi, Maria Luisa Ratto, Emily R. Cliff, Gabrielle Nattenberg, Sara Bianchi, Silvia Becca, Heather Klug, et al.D3025 Quick-RNA MiniPrep Kit,Zymo Research,Cat# ZYR1055 Stranded mRNA Prep Ligation Kit,Illumina,Cat# 20040534 TruSeq RNA UD Indexes,Illumina,Cat# 20020591 TruSeq Stranded mRNA Kit,Illumina,Cat# 20020594 Recombinant

(19) Myeloid-derived alveolar-like macrophages are a tractable model to understand the role of ontogeny in alveolar macrophage function ex vivo and in the lungsbioRxivMay 26, 2026Reham A. Ammar, Andrew J. Oliveaccording to the manufacturer’s protocol. The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the

(20) Dopamine drives persistent remodelling of the maternal brainNatureMay 20, 2026Jennifer C. O’Chan, Giuseppina Di Salvo, Ashley M. Cunningham, Sohini Dutta, Elizabeth Brindley, Benjamin H. Weekley, Winnie Chen, Rasika R. Iyer, Ethan Wan, Cindy Zhang, et al.preparation, 150 ng of mRNA per sample was used with either the Illumina Stranded mRNA Prep Kit (Illumina, 20040534) or the TruSeq RNA Library Prep Kit v2 (Illumina, RS-122-2001), according to the manufacturer’s protocols.

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Illumina Stranded mRNA Prep, Ligation (96 Samples) from Illumina

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Illumina Stranded mRNA Prep, Ligation (96 Samples) from Illumina

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