Illumina Stranded mRNA Prep, Ligation (96 Samples) 20040534 from Illumina

Product Specs
ItemIllumina Stranded mRNA Prep, Ligation (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20040534
ApplicationsmRNA-Seq, Stranded RNA-Seq
ReactivityHuman, Mouse, Rat, Bovine
TypeRNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Paternal genetic background orchestrates fetal development through tissue-specific cis-regulatory divergenceiScienceAugust 21, 2026Muhammad Arsalan Iqbal, Eduard Murani, Frieder Hadlich, Henry Reyer, Michael Oster, Nares Trakooljul, Klaus Wimmers, Siriluck Ponsuksilikit with 11 PCR-cycles of amplification according to the manufacturer’s recommendation (Illumina, Cat#20040534, Germany). The libraries were quality checked for fragment length distribution on Agilent 2100 Bioanalyzer

(2) PBRM1-dependent PBAF targeting is required for EMT and metastasis in breast cancerSci AdvJuly 31, 2026Alisha Dhiman, Mitchell G. Ayers, Guanming Jiao, Jamie L. McCuiston, Aparna B. Shinde, Saeed Salehin Akhand, Juan Jauregui-Lozano, Marco Hadisurya, Elizabeth G. Porter, W. Andy Tao, et al.isolation. Library construction was done using the Illumina Stranded mRNA prep, ligation kit (Illumina, #20040534) according to the manufacturer’s instructions and checked for quality using Qubit and Agilent Bioanalyzer

(3) Transcriptomic changes in the jerboa supraoptic nucleus during osmotic stress: PCSK1 and PTPRN changes are conserved across desert and mesic speciesJ NeuroendocrinolJuly 26, 2026Benjamin T. Gillard, Nabil Amor, Michael P. Greenwood, Abdulaziz N. Alagaili, David Murphyng of input total RNA was used in the Illumina Stranded mRNA library preparation with ligation kit (20040534) and followed with a 4 min fragmentation time. IDT for Illumina RNA UD Indexes (20,040,553, Set A) were

(4) Differential activation of NF-Κβ and HIF-1α between fetal liver-derived and myeloid-derived macrophages drives inflammatory differences following Mycobacterium abscessus infectionInfection and ImmunityJuly 14, 2026Haleigh N. Gilliland, Soledad Soverina, Kayla N. Conner, Taryn E. Vielma, Andrew J. Olive(Tecan). RNA sequencing and analysis The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the

(5) Effects of Red Onion Peel Extracts on Oxidative Stress in Caenorhabditis elegansBiomoleculesJuly 13, 2026Héctor Palacios, Miguel Ángel Rodríguez, Nerea Abasolo, Adrià Ceretó, Sara Martinez de Cripan, Camille Malterre, Kevin Leonard, Helena Torrell, Antoni Del Pino, Núria Canela, et al.Clara, CA, USA). Sequencing libraries were prepared using the Illumina Stranded mRNA Prep Kit (Cat. No. 20040534), following the manufacturer’s protocol. Paired-end sequencing (2 × 76 bp) was performed on the Illumina

(6) Tumor-derived complement C3 overexpression in STK11-mutant lung adenocarcinoma drives tumor growth and immune checkpoint inhibitor resistanceCancer Immunol ResJuly 2, 2026Sora Suzuki, Bojidar Kandar, Catrina Ting, Peter Deraska, Te-An Chen, Thejaswini Giridharan, Anm Nazmul H Khan, Kristopher Attwood, Han Yu, Kayla Catalfamo, et al.740955). Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit (Illumina 20040534). All sequencing libraries were prepared from 100ng total RNA. Library quality and quantity were verified,

(7) Targeting metabolic dependencies to reverse chemoradiotherapy resistance in colorectal cancerJ Exp Clin Cancer ResJune 23, 2026Maximilian Hellkamp, Simon Gertken, Jonas Buchloh, Julius-Leonard Hellwig, Lukas Ben Kowitzke, Ningjun Duan, Ilaria Gaspardo, Stefan Küffer, Michael Linnebacher, Philipp Ströbel, et al.for RNA-seq was performed in the STAR Hamilton NGS automation using the Illumina Stranded mRNA Prep (#20040534; Illumina) and the ID for Illumina RNA UD Indexes Set A, ligation with 96 indexes (#20091646; Illumina)

(8) A functional betalain-producing dioxygenase in Neolecta irregularis reveals an expanded evolutionary origin of the pathwayIMA FungusJune 5, 2026Pedro Martínez-Rodríguez, M. Alejandra Guerrero-Rubio, Paula Henarejos-Escudero, Samanta Hernández-García, Fernando Gandía-HerrerocDNA libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit (Illumina, catalog no 20040534), following the manufacturer’s instructions. The kit performs a PolyA capture followed by a ligation-based

(9) Combinatorial and Inducible CRISPRa/i Enables Canalized hiPSC Forward Programming and Iterative Refinement via Single-Cell GenomicsbioRxivJune 1, 2026Federica Sozza, Alberto Romano, Nicole D'Elia, Martina Terenzi, Maria Luisa Ratto, Emily R. Cliff, Gabrielle Nattenberg, Sara Bianchi, Silvia Becca, Heather Klug, et al.D3025 Quick-RNA MiniPrep Kit,Zymo Research,Cat# ZYR1055 Stranded mRNA Prep Ligation Kit,Illumina,Cat# 20040534 TruSeq RNA UD Indexes,Illumina,Cat# 20020591 TruSeq Stranded mRNA Kit,Illumina,Cat# 20020594 Recombinant

(10) Myeloid-derived alveolar-like macrophages are a tractable model to understand the role of ontogeny in alveolar macrophage function ex vivo and in the lungsbioRxivMay 26, 2026Reham A. Ammar, Andrew J. Oliveaccording to the manufacturer’s protocol. The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the

(11) Dopamine drives persistent remodelling of the maternal brainNatureMay 20, 2026Jennifer C. O’Chan, Giuseppina Di Salvo, Ashley M. Cunningham, Sohini Dutta, Elizabeth Brindley, Benjamin H. Weekley, Winnie Chen, Rasika R. Iyer, Ethan Wan, Cindy Zhang, et al.preparation, 150 ng of mRNA per sample was used with either the Illumina Stranded mRNA Prep Kit (Illumina, 20040534) or the TruSeq RNA Library Prep Kit v2 (Illumina, RS-122-2001), according to the manufacturer’s protocols.

(12) ASF1B promotes erythropoiesis by regulating the establishment and enrichment of H3.3 nucleosomesNucleic Acids ResMay 8, 2026Jinlei Liu, Xuemei Song, Lecong Zhou, Yangu Zhao, Juhyun Kim, Jun Li, Ann Dean, Xiang GuoE1–E4 population were used for RNA-seq library preparation following manufacturer’s protocol (Illumina 20040534). MEL and HUDEP2 cell RNA was isolated by TRIZOL reagent (Abclonal, rk30129). Hundred nanograms of RNAs

(13) Integrative analysis reveals generalizable human neurodegenerative disease-associated glial statesbioRxivApril 30, 2026Liam Horan-Portelance, Dominic J. Acri, Alexandra Mann, Janet Brooks, Hannah M. Bailey, J. Raphael Gibbs, Alex R. DeCasien, Mark R. CooksonMD). Library preparation for mRNA sequencing was performed using Illumina Stranded mRNA Prep Kit (Illumina #20040534) per manufacturer protocol. Quality control for sample distribution was performed with MiSeq (MiSeq

(14) Wastewater intelligence predicts the emergence of clinically-relevant and drug-resistant Candidozyma auris at healthcare facilitiesNature CommunicationsApril 18, 2026Ching-Lan Chang, Michael A. Moshi, Quang-Huy Nguyen, Junghun Oh, Ha Nguyen, Pratik Paranjape, Mohammed Abushanab, Austin J. Tang, Jose Yani Itorralba, Lauryn Massic, et al.RNase-free water. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina Catalog #20040534) with IDT RNA UD Indexes Set A (Illumina Catalog #20040553). The library preparation followed the manufacturer’s

(15) Context-dependent Interactors Regulate TDP-43 Dysfunction in ALS/FTLDbioRxivApril 10, 2026Longxin Xie, Yuehua Zhu, Bryan T. Hurtle, Matthew Wright, John L. Robinson, Jocelyn C. Mauna, Emily E. Brown, Marilyn Ngo, Cristian A. Bergmann, Jiazhen Xu, et al.DEG analysis mRNA-seq libraries were generated using the Illumina Stranded mRNA Prep Kit (Illumina, 20040534), following the protocol provided by the manufacturer. Library concentration and integrity were then

(16) Ku limits aberrant mRNA splicing promoted by intronic antisense Alu elementsbioRxivApril 2, 2026Giovanni Pascarella, Mariia Mikhova, Gargi Parkhi, Jared Godfrey, Joshua Heyza, Tomáš Janovič, Andrew Olive, Piero Carninci, Jens C Schmidt, Katheryn Meekgenerating cell lysates. RNA-seq. The Illumina Stranded mRNA Library Prep kit (Illumina, Cat no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters was used for library preparation following the

(17) BAF complexes maintain accessibility at stimulus-responsive chromatin and are required for transcriptional stimulus responsesbioRxivApril 1, 2026Alexander O. D. Gulka, Kihoon A. Kang, Ziben Zhou, David U. Gorkingenerated from 500ng total RNA for each sample using the Illumina Stranded mRNA prep kit (Illumina, 20040534) following manufacturer protocol. Library quality control and sequencing was performed as described

(18) Prolonged Loss of Oxidative Phosphorylation and Mitochondrial Mass Characterize CD66b + Leukocytes from Patients with SepsisbioRxivMarch 31, 2026Christine Rodhouse, Evan L. Barrios, Leilani Zeumer-Spataro, Leandro Balzano-Nogueira, Ruoxuan Wu, Xuanxuan Yu, Guimei Tian, Jason O. Brant, Marie-Pierre L. Gauthier, Jing Chen, et al.(Ref# 5067–5576) on TapeStation 4200 (Agilent). Illumina Stranded mRNA Prep and Ligation kit (Ref# 20040534; Illumina) were used to prepare sample libraries following the manufacturer’s recommendations. Briefly,

(19) Spatially-resolved subtype progression reveals metabolic vulnerabilities in pancreatic ductal adenocarcinomaMolecular CancerMarch 27, 2026Alexandra Maria Schmitt, Fabio Bennet Gätje, Michelle Yi Ran Tran, Teona Midelashvili, Xingbo Xu, Johannes Robert Fleischer, Henrik Spahn, Kelvin Lai, Tobias Tacke, Sara Younes, et al.performed on the STAR Hamilton NGS automation platform using the Illumina Stranded mRNA Prep Kit (Cat. No. 20040534) together with the Illumina RNA UD Indexes Set A, Ligation with 96 Indexes (Cat. No. 20091646), starting

(20) Protocol for combining BMP, MEK, WNT inhibition and iNGN2 to enable rapid neuronal differentiation and regional patterning from hiPSCsSTAR ProtocolsMarch 20, 2026Carina Habich, Astrid Doering, Justine D. Manos, Miroslav Cik, Peter ReinhardtA, B, C",Illumina,"20040553, 20040554, 20040555" "Illumina Stranded mRNA Prep, Ligation",Illumina,20040534 "Lonza™ BioWhittaker™ Versene™ (EDTA), 0.02%",Thermo Fisher,BW17711E N2 Supplement (100x),Thermo Fisher,17502-048 Neurobasal medium

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Illumina Stranded mRNA Prep, Ligation (96 Samples) from Illumina

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Illumina Stranded mRNA Prep, Ligation (96 Samples) from Illumina

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