TruSeq Stranded Total RNA Library Prep Gold (48 Samples) 20020598 from Illumina

Product Specs
ItemTruSeq Stranded Total RNA Library Prep Gold (48 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20020598
ApplicationsTotal RNA-Seq, Whole-transcriptome sequencing
ReactivityHuman, Mouse, Rat
TypeRNA Library Prep Kit
Quantity48 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) SenCat: Cataloging human cell senescence through multi-omic profiling of multiple senescent primary cell typesMol CellJune 11, 2026Carlos Anerillas, Gisela Altés, Katarina Gresova, Dimitrios Tsitsipatis, Krystyna Mazan-Mamczarz, Reema Banarjee, Ana S.G. Cunningham, Martin Salamini-Montemurri, Jen-Hao Yang, Rachel Munk, et al.preparation following the Illumina TruSeq Stranded Total RNA Library Preparation Kit protocol (Illumina, Cat# 20020598). Briefly, after rRNA depletion and cDNA generation, the cDNAs were subjected to 3’-end adenylation,

(2) Functional heterogeneity in trophoblast stem cells derived from recurrent pregnancy loss products of conceptionMolecular Human ReproductionMay 29, 2026Qi Fu, Keisuke Kozai, Xingrao Ke, Joseph M Varberg, Sheng Xia, Ashley S Howard, Michael Lydic, Kristin Holoch, Courtney Marsh, Elin Grundberg, et al.4200. Total RNA (500 ng) was processed using the Illumina TruSeq Stranded Total RNA Library Prep Gold (20020598, Illumina) in the following steps: (i) ribosomal RNA (rRNA) depletion using biotinylated, target-specific

(3) RAG1-Independent Atypical B cell Development in Chickens: Redefining the Role of RAG1 in Avian ImmunologyiScienceApril 17, 2026Seung Je Woo, Kyung Youn Lee, Jin Lee Lee Kim, Tae Hyun Kim, Jae Yong HanQuant-iT™ RiboGreen® RNA Assay Kit,Invitrogen,Cat#R11490 Illumina TruSeq Stranded Total RNA Library Prep Gold Kit,Illumina,Cat#20020598 ,, Deposited data,Deposited data,Deposited data ,, E18 chicken bursa single cell RNA seq data,In

(4) Dissecting PGE2-driven inhibition of T cell activation using single-cell multi-omic and inflammatory bowel disease genetic association analysisNature CommunicationsApril 7, 2026Zhongli Xu, Shiyue Tao, Site Feng, Xiangyu Ye, Ting Wang, Haoran Hu, Elisa Heidrich-O’Hare, Wei Chen, Richard H. DuerrInvitrogen). For RNA-seq library preparation, the TruSeq Stranded Total RNA Library Prep Gold (48 Samples) (20020598, Illumina) was used according to the manufacturer’s protocol. Library pools containing up to 4 paired

(5) Comprehensive Transcriptome Profiling of Sporadic Medullary Thyroid CarcinomasScientific DataJanuary 15, 2026Domenico Palumbo, Viola Melone, Luigi Palo, Fabio Russo, Domenico Rocco, Caterina Mian, Susi Barollo, Simona Censi, Loris Bertazza, Maria Chiara Zatelli, et al.instructions. RNA sequencing Libraries preparation was performed by using the TruSeq Stranded Total RNA kit (Cat. 20020598, Illumina) according to manufacturer’s guidelines and starting from 200 ng of input material. Libraries

(6) Non-canonical Metatranscriptomic analysis of COVID-19 and Dengue reveals an expanded microbial and AMR landscape in COVID-19 mortality patientsPLOS PathogensNovember 19, 2025Aanchal Yadav, Raiyan Ali, Priti Devi, Pallawi Kumari, Jyoti Soni, Garima, Bansidhar Tarai, Sandeep Budhiraja, Uzma Shamim, Rajesh PandeyCOVID-19 was performed using the Illumina TruSeq Stranded Total RNA Library Prep Gold (Illumina, Cat. No. 20020598). Before proceeding with cDNA synthesis for downstream library preparation, the depletion of cytoplasmic

(7) Ether-lipids accumulation promotes hepatocellular carcinoma progression linked to PPARα deficiencyJournal of Biomedical ScienceSeptember 11, 2025Pei-Yin Liao, Wen-Jen Lin, Pei-Chun Shen, Cian-Ru Yang, Ying-Chun Yu, Chun-Chieh Yeh, Long-Bin Jeng, Hsieh-Chou Lai, Wei-Chung Cheng, Wen-Lung Mathe Illumina's official protocol. Illumina's TruSeq Stranded Total RNA Library Prep Gold Kit (Cat. 20020598) was used for library construction, followed by AMPure XP beads (Beckman Coulter, USA) size selection.

(8) Human lncRNAs NEAT1 and MALAT1 regulate the tumor microenvironment in lung cancer PDX models in athymic nude miceSci RepJuly 2, 2025Min-Shiau Hsieh, Meng-Xian Lin, Bing-Ying Ho, Jong-Kai Hsiaoemploying the RNA 6000 Nano kit. Library construction was performed using the same Illumina kit (Cat. No. 20020598), and libraries were size-selected using AMPure XP beads (Beckman Coulter, USA). Sequencing was conducted

(9) CREPT is required for the metastasis of triple-negative breast cancer through a co-operational-chromatin loop-based gene regulationMol CancerJune 10, 2025Jianghua Li, Lu Xu, Jiayu Wang, Xuning Wang, Yuting Lin, Alex Yutian Zou, Fangli Ren, Yinyin Wang, Jun Li, Zhijie ChangPureLink™ Genomic DNA Mini Kit,K182001,Thermofisher QIAquick PCR Purification Kit,28104,QIAGEN TruSeq Stranded Total RNA Gold sequencing kit,20020598,Illumina Zymo clean & concentrate kit,D4003,Zymo Research TruePrep DNA Library Prep Kit V2 for Illumina,TD501,Vazyme TruePrep

(10) Alzheimer's Disease Risk Gene SORL1 Promotes Receptiveness of Human Microglia to Pro-Inflammatory StimuliGliaApril 1, 2025Ovesen PL, Juul-Madsen K, Telugu NS, Schmidt V, Frahm S, Radbruch H, Louth EL, Korshøj AR, Heppner FL, Diecke S, et al.create RNA‐seq libraries using the Illumina TruSeqTM Stranded Total RNA Library Prep Gold (Illumina, #20020598). Libraries were sequenced on the Illumina NovaSeq 6000 platform in paired‐end 100 nt mode, aiming at

(11) The translational landscape of reactive astrocytes reveals the impact of eIF2B-mediated dysregulation in VWM diseaseNAR Molecular MedicineMarch 29, 2025Shir Mandelboum, Liat Lev-Ari, Andrea Atzmon, Melisa Herrero, Naama Brezner, Daniel Benhalevy, Orna Elroy-Steinpoly(A)-selected RNA, libraries were generated using TruSeq Stranded Total RNA Library Prep Gold (Illumina #20020598). For both Ribo-seq and RNA-seq, sequencing was performed using Illumina NextSeq 550 platform using

(12) Senataxin prevents replicative stress induced by the Myc oncogeneCell Death DisMarch 19, 2025Silvia Sberna, Marco Filipuzzi, Nicola Bianchi, Ottavio Croci, Federica Fardella, Chiara Soriani, Sara Rohban, Sara Carnevali, Alessandra Alberta Albertini, Nicola Crosetto, et al.EU-RNA were used for library construction by TruSeq Stranded Total RNA by Illumina (Illumina, cat. no. 20020598). Libraries were sequenced on a Novaseq 6000 sequencer (50 bp, paired-ends, 35 millions reads). Breaks

(13) A first-in-human clinical study of an allogenic iPSC-derived corneal endothelial cell substitute transplantation for bullous keratopathyCell reports. MedicineJanuary 21, 2025Hirayama M, Hatou S, Nomura M, Hokama R, Hirayama OI, Inagaki E, Aso K, Sayano T, Dohi H, Hanatani T, et al.STEMdiff Trilineage Differentiation Kit,STEMCELL TECHNOLOGIES,Cat#05230 TruSeq Stranded Total RNA Library Prep Gold,Illumina,Cat#20020598 ,, Deposited data,Deposited data,Deposited data ,, WGS and RNA-seq,This manuscript,"EGA accession

(14) Echinococcus granulosus sensu lato promotes osteoclast differentiation through DUSP4-MAPK signaling in osseous echinococcosisFrontiers in microbiologyJanuary 1, 2025Sun H, Liu Y, Huang Y, Xiong K, Zhang Z, Wang W, Dai Y, Li J, Li Q, Wang S, et al.strand-specific libraries were constructed with Illumina TruSeq Stranded Total RNA Library Prep Kit (20020598) using 200 ng RNA as template. Gene expression quantification was performed using DESeq2 v1.38.3, with

(15) PALB2-mutated human mammary cells display a broad spectrum of morphological and functional abnormalities induced by increased TGFβ signalingCellular and Molecular Life SciencesDecember 1, 2024Hanna Tuppurainen, Niina Laurila, Marjut Nätynki, Leila Eshraghi, Anna Tervasmäki, Louisa Erichsen, Claus Storgaard Sørensen, Katri Pylkäs, Robert Winqvist, Hellevi PeltoketoLibraries were prepared from 100 ng of RNA using TruSeq® Stranded Total RNA LT Gold kit (Illumina #20020598). The quantity and quality of the libraries were assessed using Bioanalyzer 2100 with DNA 1000 Kit (Agilent Technologies

(16) Cryopreservation of mesenchymal stem/stromal cells using a DMSO-free solution is comparable to DMSO-containing cryoprotectants: results of an international multicenter PACT/BEST collaborative studyCytotherapyDecember 1, 2024Theodros Mamo, Cheryl A. Cox, Connor Demorest, Magali J. Fontaine, Allison Hubel, Linda Kelley, Aisha Khan, Denese C. Marks, Shibani Pati, Jo-Anna Reems, et al.Clara, CA, United States). cDNA libraries were generated using TruSeq Stranded Total RNA kit (Cat # 20020598, Illumina, San Diego, CA, United States) according to its protocol and sequenced on Illumina NextSeq 550

(17) Direct mitochondrial import of lactate supports resilient carbohydrate oxidationbioRxivOctober 12, 2024Ahmad A. Cluntun, Joseph R. Visker, Jesse N. Velasco-Silva, Marisa J. Lang, Luis Cedeño-Rosario, Thirupura S. Shankar, Rana Hamouche, Jing Ling, Ji Eon Kim, Ashish G. Toshniwal, et al.libraries were prepared as described using the Illumina TruSeq Stranded Total RNA Library Prep Gold kit (20020598) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an Agilent Technologies

(18) Enhancing mitochondrial pyruvate metabolism ameliorates ischemic reperfusion injury in the heartJCI InsightSeptember 10, 2024Joseph R. Visker, Ahmad A. Cluntun, Jesse N. Velasco-Silva, David R. Eberhardt, Luis Cedeño-Rosario, Thirupura S. Shankar, Rana Hamouche, Jing Ling, Hyoin Kwak, J. Yanni Hillas, et al.libraries were prepared as described using the Illumina TruSeq stranded Total RNA Library Prep Gold kit (20020598) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an Agilent Technologies

(19) A simple and scalable zebrafish model of Sonic hedgehog medulloblastomaCell ReportsAugust 27, 2024Mattie J. Casey, Priya P. Chan, Qing Li, Ju-Fen Zu, Cicely A. Jette, Missia Kohler, Benjamin R. Myers, Rodney A. Stewart5067–5579 and 5067–5580). The Illumina TruSeq Stranded Total RNA kit with Ribo-Zero Gold (Illumina 20020598) was used for library preparation. Sequencing libraries were chemically denatured and applied to Illumina

(20) Amino acids trigger MDC-dependent mitochondrial remodeling by altering mitochondrial functionbioRxivJuly 13, 2024Nidhi Raghuram, Adam L. HughesRNA sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Gold kit (20020598) with TruSeq RNA UD Indexes (20022371). Purified libraries were qualified on an Agilent Technologies

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TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina

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TruSeq Stranded Total RNA Library Prep Gold (48 Samples) from Illumina

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