TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples) 20020597 from Illumina

Product Specs
ItemTruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20020597
ApplicationsTotal RNA-Seq, Whole-transcriptome sequencing
ReactivityHuman, Mouse, Rat
TypeRNA Library Prep Kit
Quantity96 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Patient-derived pediatric brain tumor orthotopic xenografts and tumor organoids faithfully recapitulate primary tumorsScience AdvancesApril 17, 2026Justin S. Williams, Dana M. Farmer, Qianqian Li, Jake D. Friske, Jose Grenet, Sarah Robinson, Kimberly S. Mercer, Vanshita Goel, Laura Janke, Liusheng He, et al.(Thermo Fisher Scientific, #Q10211). Total RNA libraries were prepared using the TrueSeq Stranded Total RNA kit (Illumina, #20020597). Each RNA sample was sequenced using a paired-end approach with the Illumina NovaSeq 6000 platform

(2) IGFBP3 repression driven by inflammation links air pollution to placental and developmental defectsEMBO Molecular MedicineMarch 24, 2026Sunil Singh, Isha Goel, Anubhuti Rana, Anamta Gul, Javed A Quadri, Asit Ranjan Mridha, Lakshay Malhotra, Neha Kashyap, Baburajan Radha, Arnab Nayek, et al.Kit,Epigentek,P-3100-96 TUNEL In Situ Apoptosis Kit,Elabscience,E-CK-A331 TrueSeq Stranded total RNA Kit,Illumina,20020597 Qubit 4.0 fluorometer,Thermofisher,Q33238 Tapestation 4150,Agilent, D1000 screentapes,Agilent,5067-5582 Illumina

(3) microRNA-422a promotes HIV replication and innate immune evasion by targeting MECP2Molecular Therapy Nucleic AcidsMarch 12, 2026Li Du, Jean-Noël Billaud, Sushama Telwatte, Nikhila Kadiyala, Prerna Dabral, Mohamed S. Bouzidi, John Guatelli, Steven A. Yukl, Satish K. PillaicDNA libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit (Illumina, 20020597), and sequencing was performed on the Illumina Nextseq 550 Platform, generating 75 bp paired-end reads.

(4) Protocol for retinal stress culture and single-cell analysisSTAR ProtocDecember 12, 2025Jackie L. Norrie, Cody Ramirez, Michael A. DyerHigh Sensitivity Screentape,Agilent,5067–5584 TruSeq Stranded Total RNA Library Prep Kit,Illumina,20020597 ,, Deposited data,Deposited data,Deposited data ,, RNA-sequencing data,This paper,GSE264571 Single

(5) Integrative multi-omics analysis in vivo identifies influenza virus host factorsiScienceNovember 21, 2025Shriver LP, Polacco BJ, Eckhardt M, Chang MW, Martínez-Romero C, Adkins-Travis K, Cahalane C, Haas K, Stein DA, Fourati S, et al.commercial assays,Critical commercial assays ,, TruSeq Stranded Total RNA Library Prep Kit,Illumina,Cat# 20020597 "Dharmacon, siGENOME siRNA SMARTpool cherry picked pre-designed library",Horizon Discovery,N/A PTMScan

(6) Circular RNA profiling identifies circ_0001522, circ_0001278, and circ_0001801 as predictors of unfavorable prognosis and drivers of triple-negative breast cancer hallmarksCell Death DiscovJuly 9, 2025Dania Awata, Vishnubalaji Radhakrishnan, Hibah Shaath, Ramesh Elango, Sameera Rashid, Mohammed Akhtar, Thasni Karedath Abdul Azis, Ikhlak Ahmed, Khalid Ouararhni, Ammira S. Al-Shabeeb Akil, et al.sequencing Total RNA was used to prepare libraries employing the TruSeq Stranded Total RNA Library Kit (Cat no. 20020597, Illumina Inc., San Diego, CA, USA) according to the manufacturer’s protocol. Briefly, 500 ng of total

(7) Circular RMST cooperates with lineage-driving transcription factors to govern neuroendocrine transdifferentiationCancer CellMay 12, 2025Mona Teng, Jiacheng Guo, Xin Xu, Xinpei Ci, Yulin Mo, Yakup Kohen, Zuyao Ni, Sujun Chen, Wang Yuan Guo, Martin Bakht, et al.used for library construction with the TruSeq stranded total RNA library prep kit (Illumina, cat. #20020597) following manufacturer’s protocol. In brief, ribosomal RNA was first depleted and the remaining RNA

(8) KLC3 activates PI3K/AKT signaling and promotes ovarian cancer cell proliferation and migration through COL3A1Oncology ReportsApril 16, 2025Jing Yang, Rongxia He, Xiaoling Zhang, Xing Wang, Min Liu, Xiaolong Liu, Yulan Liwas used to construct cDNA libraries using the TruSeq Stranded Total RNA Library Prep Kit (cat. no. 20020597; Illumina, Inc.), according to the manufacturer's protocol. RNA was fragmented and subjected to two

(9) Latent epigenetic programs in Müller glia contribute to stress and disease response in the retinaDevelopmental CellApril 1, 2025Jackie L. Norrie, Marybeth S. Lupo, Danielle R. Little, Abbas Shirinifard, Akhilesh Mishra, Qiong Zhang, Natalie Geiger, Daniel Putnam, Nadhir Djekidel, Cody Ramirez, et al.Single Cell 3' Kit v3,10x Genomics,PN-1000075 TruSeq Stranded Total RNA Library Prep Kit,Illumina,20020597 Deposited Data,Deposited Data,Deposited Data RNA-sequencing Data,This paper,GSE264571 Single Cell

(10) ANGPTL3 regulates the peroxisomal translocation of SmarcAL1 in response to cell growth statesSci RepFebruary 11, 2025Taylor Hanta Nagai, Taiji Mizoguchi, Yanyan Wang, Amy Deik, Kevin Bullock, Clary B. Clish, Yu-Xin XuRNA-seq analyses, libraries were generated using TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (20020597, Illumina). Sequencing reads with 75 bp paired ends were generated on an Illumina HiSeq 2500 platform

(11) Isolating high-quality RNA for RNA-Seq from 10-year-old blood samplesScientific ReportsDecember 28, 2024Charlene Portelli, Elisa Seria, Ritienne Attard, Mitra Barzine, Eva M. Esquinas-Roman, Francesca Borg Carbott, Karen Cassar, Matthew Vella, Brendon P. Scicluna, Jean-Paul Ebejer, et al.out using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Globin Human kit (catalogue number 20020597). This kit removes ribosomal RNA and globin mRNA. Sequencing was carried out on a NovaSeq 6000, with

(12) Protocol to establish an accelerated murine model for Helicobacter-induced gastric cancerSTAR ProtocolsSeptember 20, 2024Prerna Bali, Ivonne Lozano-Pope, Jonathan Hernandez, Monica V. Estrada, Christopher Benner, Marygorret Obonyo,Thermo Fisher Scientific,4368814 Illumina TruSeq stranded total RNA sample prep kit with Ribo-Zero,Illumina,20020597 ,, Deposited data,Deposited data,Deposited data ,, GEO accession number: GSE250438,"Bali et al.,

(13) Bridging the gap: Multi‐omics profiling of brain tissue in Alzheimer's disease and older controls in multi‐ethnic populationsAlzheimer's & DementiaAugust 30, 2024Joseph S. Reddy, Laura Heath, Abby Vander Linden, Mariet Allen, Katia de Paiva Lopes, Fatemeh Seifar, Erming Wang, Yiyi Ma, William L. Poehlman, Zachary S. Quicksall, et al.Scientific) and the Agilent Fragment Analyzer. Using Illumina's TruSeq Stranded Total RNA reagent kit (Cat #20020597) and the Illumina Ribo‐Zero Plus rRNA Depletion kit (Cat #20037135), libraries were prepared according

(14) CRISPR screening uncovers a long-range enhancer for ONECUT1 in pancreatic differentiation and links a diabetes risk variantCell ReportsAugust 27, 2024Samuel Joseph Kaplan, Wilfred Wong, Jielin Yan, Julian Pulecio, Hyein S. Cho, Qianzi Li, Jiahui Zhao, Jayanti Leslie-Iyer, Jonathan Kazakov, Dylan Murphy, et al.,Thermo Fisher Scientific,00-5523-00 Arima-Hi-C kit,Arima,A510008 Monarch® HMW DNA Extraction Kit,NEB,T3050 TruSeq Stranded Total RNA,Illumina,20020597 Zero Blunt TOPO PCR Cloning Kit,Thermo Fisher Scientific,450245 Deposited data,Deposited data,Deposited

(15) Radiotolerance of N-cycle bacteria and their transcriptomic response to low-dose space-analogue ionizing irradiationiScienceMay 17, 2024Tom Verbeelen, Celia Alvarez Fernandez, Thanh Huy Nguyen, Surya Gupta, Baptiste Leroy, Ruddy Wattiez, Siegfried E. Vlaeminck, Natalie Leys, Ramon Ganigué, Felice MastroleoRibo-Zero Plus rRNA Depletion Kit,Illumina,Cat#20037135 Illumina TruSeq Stranded Total RNA,Illumina,Cat#20020597 QiAMP DNA Mini kit,Qiagen,Cat#51304 QuantiNova SYBR Green RT-PCR kit,Qiagen,Cat#208154 ,, Deposited

(16) Hadza Prevotella require diet-derived microbiota-accessible carbohydrates to persist in miceCell ReportsMarch 21, 2024Rebecca H. Gellman, Matthew R. Olm, Nicolas Terrapon, Fatima Enam, Steven K. Higginbottom, Justin L. Sonnenburg, Erica D. Sonnenburg"RiboMinus™ Transcriptome Isolation Kit, bacteria",Invitrogen,Cat#K155004 TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat,Illumina,Cat#20020597 NovaSeq SP Flow Cell,Illumina,NovaSeq 6000 System ,, Deposited data,Deposited data,Deposited data ,, Hadza

(17) TRIF-IFN-I pathway in Helicobacter-induced gastric cancer in an accelerated murine disease model and patient biopsiesiScienceMarch 8, 2024Prerna Bali, Ivonne Lozano-Pope, Jonathan Hernandez, Monica V. Estrada, Maripat Corr, Michael A. Turner, Michael Bouvet, Christopher Benner, Marygorret ObonyoKit,Thermo Fisher,4368814 Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero,Illumina,20020597 ,, Deposited data,Deposited data,Deposited data ,, GEO accession number: GSE250438,This paper,GSE250438 ,, Experimental models

(18) Chromatin regulator SMARCAL1 modulates cellular lipid metabolismCommun BiolDecember 21, 2023Taylor Hanta Nagai, Chrissy Hartigan, Taiji Mizoguchi, Haojie Yu, Amy Deik, Kevin Bullock, Yanyan Wang, Debra Cromley, Monica Schenone, Chad A. Cowan, et al.RNA-seq analyses, libraries were prepared using TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (20020597, Illumina). Sequencing reads with 75 bp paired ends were generated on an Illumina HiSeq 2500 platform

(19) Carbon source–dependent capsule thickness regulation in Streptococcus pneumoniaeFrontiers in Cellular and Infection MicrobiologyNovember 29, 2023Joel P. Werren, Nadja Mostacci, Ilche Gjuroski, Lalaina Holivololona, Lukas J. Troxler, Lucy J. Hathaway, Julien Furrer, Markus HiltyRNA were made into cDNA libraries using a TruSeq Stranded Total RNA Human/Mouse/Rat Kit (Illumina, 20020597) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, 20022371). This was done according to the reference

(20) An integrated systems biology approach reveals differences in formate metabolism in the genus MethanothermobacteriScienceOctober 20, 2023Isabella Casini, Tim McCubbin, Sofia Esquivel-Elizondo, Guillermo G. Luque, Daria Evseeva, Christian Fink, Sebastian Beblawy, Nicholas D. Youngblut, Ludmilla Aristilde, Daniel H. Huson, et al.Agilent RNA 6000 Nano Kit,Agilent Technologies,Cat# 5067-1511 TruSeq™ Stranded Total RNA Kit,Illumina,Cat# 20020597 Illumina Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat# 20037135 ,, Deposited data,Deposited data,Deposited

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TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples) from Illumina

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TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples) from Illumina

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