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TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples) from Illumina
Be the first to write a review!
Citations:
(65)
Supplier Page
Supplier Page from
Illumina for
TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
Description
Whole-transcriptome stranded RNA-Seq library prep with Ribo-Zero rRNA depletion for human, mouse, and rat samples; 96-sample format
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
20020597
Applications
Total RNA-Seq, Whole-transcriptome sequencing
Reactivity
Human, Mouse, Rat
Type
RNA Library Prep Kit
Quantity
96 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (65)
(21)
Chromatin regulator SMARCAL1 modulates cellular lipid metabolism
Commun Biol
December 21, 2023
Taylor Hanta Nagai, Chrissy Hartigan, Taiji Mizoguchi, Haojie Yu, Amy Deik, Kevin Bullock, Yanyan Wang, Debra Cromley, Monica Schenone, Chad A. Cowan,
et al.
RNA-seq
analyses, libraries were prepared using TruSeq® Stranded Total
RNA Library Prep
Human
/
Mouse
/
Rat
(
20020597
,
Illumina). Sequencing
reads with 75 bp paired ends were generated on an
Illumina
HiSeq 2500 platform
(22)
Carbon source–dependent capsule thickness regulation in Streptococcus pneumoniae
Frontiers in Cellular and Infection Microbiology
November 29, 2023
Joel P. Werren, Nadja Mostacci, Ilche Gjuroski, Lalaina Holivololona, Lukas J. Troxler, Lucy J. Hathaway, Julien Furrer, Markus Hilty
RNA were made into cDNA libraries using a TruSeq Stranded Total RNA
Human
/
Mouse
/
Rat
Kit (
Illumina
,
20020597
) with IDT for Illumina TruSeq RNA UD Indexes (Illumina, 20022371). This was done according to the reference
(23)
An integrated systems biology approach reveals differences in formate metabolism in the genus Methanothermobacter
iScience
October 20, 2023
Isabella Casini, Tim McCubbin, Sofia Esquivel-Elizondo, Guillermo G. Luque, Daria Evseeva, Christian Fink, Sebastian Beblawy, Nicholas D. Youngblut, Ludmilla Aristilde, Daniel H. Huson,
et al.
Agilent RNA 6000 Nano Kit
,
Agilent Technologies
,Cat#
5067-1511
TruSeq™ Stranded Total RNA Kit,Illumina,Cat#
20020597
Illumina
Ribo-Zero Plus rRNA Depletion Kit,
Illumina
,Cat#
20037135
,, Deposited data,Deposited data,Deposited
(24)
The NSL complex is required for piRNA production from telomeric clusters
Life science alliance
September 1, 2023
Iyer SS, Sun Y, Seyfferth J, Manjunath V, Samata M, Alexiadis A, Kulkarni T, Gutierrez N, Georgiev P, Shvedunova M,
et al
.
(1 μg) was used to prepare libraries for
sequencing
. The TrueSeq Stranded Total
RNA Library Prep
(#
20020597
;
Illumina
) was used to generate libraries using the manufacturer’s recommendations. Ribosomal RNAs were
(25)
Transcriptome from Paired Samples Improves the Power of Comprehensive COVID-19 Host-Viral Characterization
International Journal of Molecular Sciences
August 23, 2023
Ognjen Milicevic, Ana Loncar, Dzihan Abazovic, Marija Vukcevic, Dragana Despot, Tatjana Djukic, Vladimir Djukic, Andjela Milovanovic, Nikola Panic, Nemanja Plecic,
et al.
from total RNA using poly-T oligo-attached magnetic beads after rRNA removal (
Illumina
, Kit Cat. No.
20020597
). After fragmentation, the first strand of cDNA was synthesized using random hexamer primers, followed
(26)
Human galectin-9 potently enhances SARS-CoV-2 replication and inflammation in airway epithelial cells
Journal of molecular cell biology
August 3, 2023
Du L, Bouzidi MS, Gala A, Deiter F, Billaud JN, Yeung ST, Dabral P, Jin J, Simmons G, Dossani ZY,
et al
.
cDNA libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit (
Illumina
,
20020597
), and
sequencing
was performed on the
Illumina
Nextseq 550
Platform, generating 75 bp paired-end reads.
(27)
Transcriptome profiling and network enrichment analyses identify subtype-specific therapeutic gene targets for breast cancer and their microRNA regulatory networks
Cell Death & Disease
July 12, 2023
Ramesh Elango, Sameera Rashid, Radhakrishnan Vishnubalaji, Reem Al-Sarraf, Mohammed Akhtar, Khalid Ouararhni, Julie Decock, Omar M. E. Albagha, Nehad M. Alajez
RNA sequencing
Total RNA was used for library preparation using TruSeq Stranded Total RNA Library (Cat no.
20020597
,
Illumina Inc
., San Diego, CA, USA) following the manufacturer’s protocol. Briefly, 500 ng of total
(28)
A Protocol for Low-Input RNA-Sequencing of Patients with Febrile Neutropenia Captures Relevant Immunological Information
International Journal of Molecular Sciences
June 16, 2023
Victoria Probst, Lotte Møller Smedegaard, Arman Simonyan, Yuliu Guo, Olga Østrup, Kia Hee Schultz Dungu, Nadja Hawwa Vissing, Ulrikka Nygaard, Frederik Otzen Bagger
Protocol Standard
RNA-sequencing
was performed using a TruSeq Stranded
RNA Library Preparation
Kit (cat no.
20020597
,
Illumina
, San Diego, CA, USA) (hereafter termed “standard protocol”) according to the manufacturer’s
(29)
Transcriptional and metabolic programs promote the expansion of follicular helper T cells in lupus-prone mice
iScience
May 19, 2023
Gong M, Choi SC, Park YP, Zou X, Elshikha AS, Gerriets VA, Rathmell JC, Mohamazadeh M, Morel L.
commercial
assays
,, RNeasy Kit,
Qiagen
,
74004
TruSeq Stranded RNA Sample Preparation Kit,
Illumina
,
20020597
PicoGreen
assay
,
Thermo Fisher
,
P7589
CD4
T Cell
Isolation Kit,
Miltenyi Biotec
,
130-104-454
Naïve CD4
(30)
Genomic–transcriptomic evolution in lung cancer and metastasis
Nature
April 20, 2023
Carlos Martínez-Ruiz, James R. M. Black, Clare Puttick, Mark S. Hill, Jonas Demeulemeester, Elizabeth Larose Cadieux, Kerstin Thol, Thomas P. Jones, Selvaraju Veeriah, Cristina Naceur-Lombardelli,
et al.
using an
Illumina
TruSeq Stranded Total RNA
Human
/
Mouse
/
Rat
ribo-depletion library preparation kit (
20020597
), and
PCR
was amplified for 15 cycles according to the manufacturer’s guidelines. Final libraries were
(31)
A multiomics analysis of direct interkingdom dynamics between influenza A virus and Streptococcus pneumoniae uncovers host-independent changes to bacterial virulence fitness
PLoS pathogens
December 1, 2022
Platt MP, Lin YH, Penix T, Wiscovitch-Russo R, Vashee I, Mares CA, Rosch JW, Yu Y, Gonzalez-Juarbe N.
sequencing
.
Sequencing
The library was prepped using the TruSeq Stranded Total RNA by
Illumina
(PN.
20020597
). Samples were sequenced on the Illumina Novaseq 6000.
RNA-seq
analysis Raw paired-end reads in FASTQ
(32)
Profiling mRNA, miRNA and lncRNA expression changes in endothelial cells in response to increasing doses of ionizing radiation
Sci Rep
November 19, 2022
Sunita Chopra, Uma Shankavaram, Michelle Bylicky, Juan Dalo, Kevin Scott, Molykutty J. Aryankalayil, C. Norman Coleman
(Novogene, Sacramento, CA) using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, US; catalogue #
20020597
) with
Illumina
Ribo-Zero Plus rRNA Depletion kit (
Illumina
, US; catalogue #
20037135
) following manufacturer’s
(33)
Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples
iScience
November 18, 2022
Tom Verbeelen, Rob Van Houdt, Natalie Leys, Ramon Ganigué, Felice Mastroleo
Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat#
20037135
Illumina
TruSeq Stranded Total RNA,
Illumina
,Cat#
20020597
,, Deposited data,Deposited data,Deposited data ,,
RNA-seq
data of
Nitrosomonas europaea
,This paper;
(34)
Genome-wide fetalization of enhancer architecture in heart disease
Cell Reports
September 20, 2022
Cailyn H. Spurrell et al.
Qubit dsDNA HS Assay Kit
,
Life Technologies
,
Q32851
TruSeq Stranded Total RNA with Ribo-Zero
Human
/
Mouse
/
Rat
kit,
Illumina
,
20020597
,, Deposited data,Deposited data,Deposited data ,, Raw and analyzed data,This paper,GEO: GSE126573 ,,
Experimental models
(35)
Genetic and microenvironmental intra-tumor heterogeneity impacts colorectal cancer evolution and metastatic development
Communications Biology
September 9, 2022
Daniel Sobral, Marta Martins, Shannon Kaplan, Mahdi Golkaram, Michael Salmans, Nafeesa Khan, Raakhee Vijayaraghavan, Sandra Casimiro, Afonso Fernandes, Paula Borralho,
et al.
library preparation.
Whole transcriptome sequencing
Illumina TruSeq™ Stranded Total RNA (
Illumina
, PN
20020597
) with 100 ng input per RNA sample was used for generating whole transcriptome libraries according to
(36)
Identification of a neural development gene expression signature in colon cancer stem cells reveals a role for EGR2 in tumorigenesis
iScience
July 15, 2022
Joseph L. Regan et al.
kit,
Qiagen
,ID:
74136
Sensiscript RT kit
,
Qiagen
,ID:
205213
TruSeq
RNA Library Preparation
Kit,
Illumina
,
20020597
CellTiter-Glo
Cell Viability Assay
,Fisher Scientific,G7571 ,, Deposited data,Deposited data,Deposited
(37)
Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability
SSRN Electronic Journal
March 30, 2022
Diana Zatreanu, Richard Mitter, Emanuela Tumini, Hannah Williams, Lea Gregersen, Stefania Roma, Aengus Stewart, Andres Aguilera, Jesper Svejstrup
kit
Illumina
20020595
Critical Commercial
Assays
Strand specific TruSeq total RNA kit
Illumina
20020597
KAPA
RNA
Hyper prep
Illumina KR1350 Taqman Reverse Transcriptase Reagents
ThermoFisher Scientific
N8080234
Comet
(38)
Non-cell-autonomous disruption of nuclear architecture as a potential cause of COVID-19-induced anosmia
Cell
March 17, 2022
Marianna Zazhytska et al.
assays
,Critical commercial
assays
,Critical commercial
assays
,, Truseq RNA Library Prep Kit v2,
Illumina
,
20020597
Direct-zol RNA kits from Zymo Research,
Zymo Research
,cat no.
R2052
RNAscope
Multiplex
Fluorescenct
(39)
Virome characterization of game animals in China reveals a spectrum of emerging pathogens
Cell
March 1, 2022
Wan-Ting He, Xin Hou, Jin Zhao, Jiumeng Sun, Haijian He, Wei Si, Jing Wang, Zhiwen Jiang, Ziqing Yan, Gang Xing,
et al.
RESOURCE,SOURCE,IDENTIFIER TRIzol,
Thermofisher
,Cat. #
15596026
TruSeqTM Stranded Total RNA Sample Preparation Kit,
Illumina
,Cat. #
20020597
DNase I,TAKAR,Cat. #2270B Ribo-ZeroTM rRNA Removal Kits,
Illumina
,Cat. #
20037135
Biological samples,Biological
(40)
Predictive value of using plasma long non‑coding RNAs ANRIL and HOXA11‑AS for in‑stent restenosis
Experimental and Therapeutic Medicine
December 6, 2021
Zhijiang Jin, Hongfeng Shen, Wei Cha, Haijiang Xia, Longbin Liu
strand-specific manner from 4 µg of DNase-treated RNA using TruSeq Stranded Total RNA Library Prep kit (cat. no.
20020597
;
Illumina, Inc
.) according to the manufacturer's instructions. The samples were denatured to single-stranded
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TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples) from Illumina
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