TruSeq DNA PCR-Free Low Throughput Library Prep Kit 20015962 from Illumina

Product Specs
ItemTruSeq DNA PCR-Free Low Throughput Library Prep Kit
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog Number20015962
ApplicationsWhole-genome sequencing
ReactivityHuman, Mouse, Rat, Any
TypeDNA Library Prep Kit
Quantity24 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Common Chiffchaffs (Phylloscopus collybita) Diverge in a Genomic Region Associated With Migration Differences in Willow Warblers (Phylloscopus trochilus)Mol EcolJuly 9, 2026Violeta Caballero-Lopez, Alexander Mackintosh, Diana Ekman, Estelle Proux-Wéra, Max Lundberg, Gintaras Malmiga, Daria Shipilina, Michal Polakowski, Michaëla Berdougo, Łukasz Jankowiak, et al.sequencing libraries were prepared from 1 μg DNA with a TruSeq PCR‐free sample preparation kit (cat#20015962/3, Illumina Inc.) targeting a size of 350 bp and sequenced on Novaseq 6000 S4 (Illumina) using 1.5 sequencing

(2) Genetics of growth rate in induced pluripotent stem cellsStem Cell ReportsJune 9, 2026Brian N. Lee, Henry J. Taylor, Filippo Cipriani, Narisu Narisu, Catherine C. Robertson, Amy J. Swift, Neelam Sinha, Tingfen Yan, Lori L. Bonnycastle, Nathan Dale, et al.NISC for library preparation with the Illumina TruSeq PCR-free Sample Preparation kit (Illumina, Cat. #20015962). We sequenced libraries on the Illumina NovaSeqX platform using 25B flow cells. We downloaded genome

(3) An X-linked sex determination mechanism in cannabis and hopNature CommunicationsMay 26, 2026Sarah B. Carey, Philip C. Bentz, John T. Lovell, Laramie M. Akozbek, Zachary A. Myers, Walid Korani, Joshua S. Havill, Lillian Padgitt-Cobb, Ryan C. Lynch, Nicholas Allsing, et al.and sequencing libraries were constructed using an Illumina TruSeq DNA PCR-free library kit (Catalog #20015962) using standard protocols. Libraries were sequenced on an Illumina NovaSeq 6000 instrument using paired

(4) Structure and sequence evolution in the pennycress ( Thlaspi arvense ) pangenomeNew PhytologistApril 2, 2026Kevin A. Bird, Joanna L. Rifkin, Chloee M. McLaughlin, Avril M. Harder, Pawan Basnet, Ella Katz, Tomáš Brůna, Kerrie Barry, LoriBeth Boston, Christopher Daum, et al.libraries were prepared for all accessions using Illumina TruSeq PCRfree library prep kits (catalog no.: 20015962). In addition, Omni‐C libraries were prepared for extra scaffolding using Dovetail Omni‐C kits (catalog

(5) Chromosome-level genome assembly of Neoseiulus longispinosus (Phytoseiidae)Scientific DataMarch 6, 2026Zi-Rui Han, Li-Jiu Zheng, Lang Liang, Zheng-Pei Ye, Yue-Guan Fu, Tian-Ci Yi, Jun-Yu Cheninsert library was constructed utilising a TruSeq DNA PCR-Free Library Preparation Kit (Illumina, Cat. #20015962; San Diego, CA, USA), which was followed by paired-end sequencing on an Illumina NovaSeq. 6000 platform.

(6) Integrated Histology and Molecular Profiling of Postmortem Human Auditory and Vestibular Organs via a Poly (Methyl Methacrylate)-Based WorkflowJournal of the Association for Research in OtolaryngologyFebruary 23, 2026David Bächinger, Brock Peyton, Jacqueline Neubauer, Anbuselvan Dharmarajan, MengYu Zhu, Jennifer T. O’Malley, Venus Kallupurackal, Steven Senese, Alison Brown, Sabina Wunderlin, et al.and TV7-FF, DNA (1 µg input) was processed independently using the TruSeq PCR-Free library prep kit (#20015962, Illumina, San Diego, CA), following the manufacturer’s guidelines. Libraries were quantified with the

(7) Identification of Resistance Genes in Breast Cancer Cells Treated with Fulvestrant and Ribociclib via Retroviral Screening and Integration Site SequencingCellsJanuary 29, 2026Zhangzan Huang, Corine Beaufort, Jean Helmijr, Brian Zantboer, Giada Rozema, Camilla Muritti, Julia J. Whien, Anna Uijterwegen, Michele Massimino, John W. M. Martens, et al.prepared for all samples using the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA, Cat. No. 20015962), and pools of libraries were sequenced in paired-end form at 2 × 150 bp on a MiSeq Illumina system

(8) Integrated analysis of gut microbiota structure and metabolic function in patients with cirrhosis and refractory ascitesBiomedical ReportsJanuary 2, 2026Dianyan Chen, Sandu Liu, Ka Zhang, Ke Pan, Linsu Zhang, Deyun Shu, Jin Zhang, Dingyao Renfluorometer. Sequencing libraries were constructed using the TruSeq DNA PCR-Free Kit (Illumina, Inc; cat. no. 20015962). The final library concentration was quantified by qPCR and loaded at a final concentration of 12.5

(9) Combined somatic mutation and transcriptome analysis reveals region-specific differences in clonal architecture in human cortexCell ReportsNovember 25, 2025Vinayak V. Viswanadham, Sonia N. Kim, Emre Caglayan, Ryan N. Doan, Yanmei Dou, Sara Bizzotto, Sattar Khoshkhoo, August Yue Huang, Rebecca Yeh, Brian H. Chhouk, et al.commercial assays QIAamp DNA mini kit,Qiagen,Cat. #51304 Illumina TruSeq PCR-free preparation kit,Illumina,20015962 AmpliSeq Primer Pool and Phusion U Mastermix,Thermo Fisher Scientific,F-562 "Chromium Next GEM Single

(10) A comparison of 27 Arabidopsis thaliana genomes and the path toward an unbiased characterization of genetic polymorphismNature GeneticsSeptember 1, 2025Anna A. Igolkina, Sebastian Vorbrugg, Fernando A. Rabanal, Hai-Jun Liu, Haim Ashkenazy, Aleksandra E. Kornienko, Joffrey Fitz, Max Collenberg, Christian Kubica, Almudena Mollá Morales, et al.prepared according to the manufacturer’s instructions with either the TruSeq DNA PCR-free kit (Illumina, 20015962) or the NxSeq AmpFREE Low DNA Library kit (Lucigen, 14000-2). In total, libraries for 89 accessions

(11) Physiological plasticity in zebra finch color varieties mitigates DNA damage under oxidative stressiScienceJuly 18, 2025Cristiana I. Marques, Sol Rodríguez-Martínez, Pedro Miguel Araújo, Sandra Afonso, Mohammed Zougagh, Ángel Ríos, Miguel Carneiro, Ismael Galváncommercial assays,Critical commercial assays ,, TruSeq DNA PCR-free Library Preparation Kit,Illumina,20015962 RNeasy Mini Kit Plus,Qiagen,74134 Xpert cDNA Synthesis Kit,GRiSP,GK80.0100 ,, Deposited data,Deposited

(12) Multiple, redundant carboxylic acid transporters support mitochondrial metabolism in Plasmodium falciparumJournal of Biological ChemistryJuly 1, 2025Krithika Rajaram, Gabriel W. Rangel, Justin T. Munro, Sethu C. Nair, Rubayet Elahi, Manuel Llinás, Sean T. Priggewith 1 μg of gDNA per sample using the TruSeq DNA PCR-Free Low-Throughput Library Prep Kit (Illumina, 20015962) according to the manufacturer’s directions. The Δ2 and Δ3 libraries were sequenced on a HiSeq2500 Illumina

(13) Wild and domesticated animal abundance is associated with greater late-Holocene alpine plant diversityNat CommunApril 25, 2025Sandra Garcés-Pastor, Peter D. Heintzman, Scarlett Zetter, Youri Lammers, Nigel G. Yoccoz, Jean-Paul Theurillat, Christoph Schwörer, Andreas Tribsch, Kevin Walsh, Boris Vannière, et al.Each pool was converted into a DNA library using a modified TruSeq PCR-free library kit (Illumina, 20015962) and unique dual-indexing (Illumina, 20022370)51. The libraries were quantified by qPCR using the Library

(14) Duckweed genomes and epigenomes underlie triploid hybridization and clonal reproductionCurrent BiologyApril 21, 2025Evan Ernst, Bradley Abramson, Kenneth Acosta, Phuong T.N. Hoang, Cristian Mateo-Elizalde, Veit Schubert, Buntora Pasaribu, Patrice S. Albert, Nolan Hartwick, Kelly Colt, et al.Lj8627 were prepared from 2μg of HMW gDNA using the Illumina TruSeq DNA PCR-Free kit (Illumina Cat#20015962) and sequenced on an Illumina MiSeq (PE 250bp, PE 300bp) or HiSeq 2500 instrument (PE 150bp). Libraries

(15) Short- and mid-term temporal variability of the human urinary microbiota: a prospective observational cohort studyBMC MicrobiolApril 16, 2025Vojtěch Tláskal, Jan Hrbáček, Vítězslav Hanáček, Petra Baránková, Pavel Čermák, Roman Zachoval, Priscila Thiago Dobbler(Thermo Fisher Scientific). Sequencing libraries were prepared using the TruSeq DNA PCR-Free Kit (Illumina, Ref. 20015962) according to manufacturer’s instructions while following official ligation protocol for the kit used.

(16) Relics of interspecific hybridization retained in the genome of a drought-adapted peanut cultivarG3: Genes, Genomes, GeneticsNovember 6, 2024Paul P Grabowski, Phat Dang, Jerry J Jenkins, Avinash Sreedasyam, Jenell Webber, Marshall Lamb, Qiong Zhang, Alvaro Sanz-Saez, Yucheng Feng, Victoria Bunting, et al.Line8, Georgia Green, and C76-16 were built using standard protocols (Illumina TruSeq PCRfree Catalog #20015962). We sequenced Line8 using a whole-genome shotgun sequencing strategy and standard sequencing protocols.

(17) Landscape of genomic structural variations in Indian population-based cohorts: Deeper insights into their prevalence and clinical relevanceHuman Genetics and Genomics AdvancesJuly 18, 2024Krithika Subramanian, Mehak Chopra, Bratati Kahalifluorometer (Thermo Fisher Scientific, Cat #Q33228). We used the TruSeq DNA PCR-Free kit (Illumina, Cat # 20015962) for library preparation as per the manufacturer’s instructions. TruSeq DNA PCR-Free offers superior

(18) Chromosome-level genome assembly and annotation of the social amoeba Dictyostelium firmibasisScientific DataJune 22, 2024Bart Edelbroek, Jonas Kjellin, Jon Jerlström-Hultqvist, Sanna Koskiniemi, Fredrik Söderbomsequencing library was prepared with 1 µg of DNA using the TruSeq PCR-free DNA sample preparation kit (cat# 20015962, Illumina), according to the TruSeq DNA PCR-free Reference Guide, using unique dual indexes (cat# 20022370,

(19) Comparative genomics of Ascetosporea gives new insight into the evolutionary basis for animal parasitism in RhizariaBMC BiologyMay 3, 2024Markus Hiltunen Thorén, Ioana Onuț-Brännström, Anders Alfjorden, Hana Pecková, Fiona Swords, Chantelle Hooper, Astrid S. Holzer, David Bass, Fabien Burkisequencing libraries were prepared from 1 µg DNA using the TruSeq PCRfree DNA sample preparation kit (cat# 20015962, Illumina Inc.) and unique dual indexes (cat#20022370, Illumina Inc.), targeting an insert size of 350

(20) Secondary bone marrow graft loss after third-party virus-specific T cell infusion: Case report of a rare complicationNat CommunMarch 29, 2024Michael D. Keller, Stefan A. Schattgen, Shanmuganathan Chandrakasan, E. Kaitlynn Allen, Mariah A. Jensen-Wachspress, Christopher A. Lazarski, Muna Qayed, Haili Lang, Patrick J. Hanley, Jay Tanna, et al.extracted and processed using the TruSeq DNA PCR-Free whole genome library preparation kit (Illumina, Cat#20015962). These regions were sequenced by massive parallel (NextGen) sequencing with 150 bp paired-end reads

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TruSeq DNA PCR-Free Low Throughput Library Prep Kit from Illumina

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TruSeq DNA PCR-Free Low Throughput Library Prep Kit from Illumina

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