Nextera XT DNA Library Preparation Kit (24 Samples) FC-131-1024 from Illumina

Product Specs
ItemNextera XT DNA Library Preparation Kit (24 Samples)
CompanyIllumina
Price Supplier Page View Company Product Page
Catalog NumberFC-131-1024
ApplicationsAmplicon sequencing, WGS, De novo sequencing, Metagenomics
ReactivityAny
TypeDNA Library Prep Kit
Quantity24 Samples

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Illumina

5200 Illumina Way

San Diego, CA 92122

United States

Phone: 858.202.4500

Company Profile

Website: http://illumina.com

(1) Axonal defasciculation is restricted to specific branching points during regeneration of the lateral line nerve in zebrafishDevelopmentAugust 15, 2026Rohan S. Roy, A. J. Hudspethof cDNA was then used to prepare libraries using the Illumina Nextera XT DNA sample preparation kit (FC-131-1024, Illumina). Libraries generated from hair cells 1 day post nerve lesion and control hair cell samples

(2) Linking Composition and Function: A Two-Year Metatranscriptomic Study of Microbial Adaptation in an Industrial Wastewater Treatment PlantFEMS Microbiology EcologyJune 18, 2026Asala Mahajna, Ranko Gacesa, Karel J Keesman, Gert-Jan W Euverink, Andreas Froemelt, Carlos J Melián, Bayu Jayawardhanasubsequently prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA; Cat. No. FC-131-1024), and sequencing was performed on the MiSeq platform at the University Medical Center Groningen Sequencing

(3) Differentiation defects reposition sebaceous glands as inflammatory instigators in the early pathogenesis of hidradenitis suppurativaFront ImmunolJune 8, 2026Xiang Chen, Jiaqi Li, Yibo Feng, Zhanyan Pan, Qiong Wu, Xinyi Xu, Qian Zhao, Tingting Hu, Guangjie Chen, Christos C. Zouboulis, et al.transcription of RNA were processed. The cDNA was amplified with the Nextera XT DNA sample Pre-Kit (FC-131-1024, Illumina, San Diego, USA) to gain sequencing libraries. Libraries of individual samples were measured

(4) Clinical severity-dependent virulence of Staphylococcus aureus from human diabetic foot ulcers drives impaired wound healing in a diabetic murine modelFront Cell Infect MicrobiolJune 5, 2026Keun Seok Seo, Lee M. Nicols, Cara Dale Palmer, K. Taylor Hellmann, D. Ashley Robinson, Alicia K. Olivier, Justin A. Thornton, Khadija Ferdous, Wilson Cooper Brookshire, Joo Youn ParkScientific, USA), and 150bp paired-end libraries were prepared with a Nextera XT DNA Library Preparation Kit (FC-131-1024, Illumina, USA). Sequencing was performed at the Molecular and Genomics Core Facility of the University

(5) Squamous-state excursions activate APOBEC3A in cancerbioRxivMay 28, 2026Josefine Striepen, Alexandra Dananberg, Aušrinė Ruzgaitė, Alyssa Hurley, Eléonore Toufektchan, Ashley Nichols, Hannah Rosenberg, Cameron Cordero, Tony M. Mertz, Roshan Xavier Norman, et al.ng of cDNA was used to prepare libraries with the Nextera XT DNA Library Preparation Kit (Illumina, FC-131–1024) in a total volume of 6.25 μL with 8–12 cycles of PCR. Indexed libraries were pooled by volume and cleaned

(6) COX2 + / PDGFRα + fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in micePhysiological ReportsMay 24, 2026Momo Goto, Tamaki Kurosawa, Hiroyuki Koike, Noriyuki Kaji, Ichiro Manabe, Taiki Mihara, Takashi Chaen, Yumiko Oishi, Masatoshi HoriSequencing (# 634888, Takara Bio, Shiga, Japan) and Nextera TX DNA Library Prep Kit for Illumina (# FC‐131‐1024, Illumina, San Diego, CA, USA). Libraries were sequenced using Illumina NovaSeq 6000 (Illumina), and

(7) UMI-guided single locus sequence typing method for phylotyping Cutibacterium acnes from skin samplesFrontiers in Cellular and Infection MicrobiologyMay 20, 2026Elody Orcel, Erwin Sentausa, Hayat Hage, Karen Louis, May Taha, Samuel Bellais, Adrien Villain, Laurent Beloeil, Steven Sijmons, Nathalie Devos, et al.subjected to Illumina library preparation using the Nextera XT DNA Library Preparation Kit (Illumina, #FC-131-1024) with minor modifications. Briefly, 5 µL of pooled DNA diluted to 0.2 ng/µL were mixed with 10 µL of

(8) Endocrine therapy reprogramming of breast cancer facilitates metastatic escape via upregulation of P-Rex1/Rac1 signallingNat CommunMay 11, 2026Kristine J. Fernandez, Ghazal Sultani, Max Nobis, Brian Gloss, Leila Eshraghi, Amy E. McCart Reed, Sarah Alexandrou, Christine Lee, Daniel L. Roden, Emily I. Jones, et al.amplified by PCR. Pooled cDNAs were then tagmented with the Nextera XT DNA Library kit (Illumina Cat# FC-131-1024) and custom primers as in ref. 96. Tagmented and multiplexed cDNA libraries were sequenced as in ref.

(9) RNase P/MRP subunits chaperone telomerase holoenzyme assembly in fission yeastEMBO ReportsApril 28, 2026Lili Pan, Valentine Patterson, Martin M Möckel, Rachel M Helston, Raymund J Wellinger, David C Zappulla, Peter Baumannequimolar ratios, and dual-indexed libraries were prepared using the Nextera XT Library Prep Kit (Illumina, FC-131-1024). Libraries were sequenced as 2×150 bp paired-end reads on a NextSeq 500 or 2000 platform. SHAPE data

(10) Two molecularly-defined neuronal types in the mammillary body govern different temporal periods during working memory maintenanceResearch (Wash D C)April 22, 2026Xinyan Guo, Lanfang Guo, Lanfang Hou, Zhenye Hou, Xiaomei Tang, Xiaomei Tang, Changdong Zeng, Changdong Jiang, Fuliang Jiang, Zhigao Xiang, et al.(Agilent 5067-4626). Sequencing libraries were prepared with Nextera XT DNA Library Prep Kit (Illumina FC-131-1024), followed by sequencing on Illumina NovaSeq (>1 million reads per sample). TopHat2 (v2.1.0) aligned

(11) Characterizing the impact of MLL fusion variants and fusion partners on leukemia plasticity using a human CRISPR-engineered MLL-rearranged leukemia modelNeoplasiaApril 18, 2026Pia Radszuweit, Rahel Fitzel, Sarah Bruestl, Thomas Hentrich, Fulya Korkmaz, Barbara Mankel, Irene González-Menéndez, Saskia Rudat, Rolf Marschalek, Estelle Erkner, et al.The final libraries were prepared by using the Nextera DNA Library Preparation Kit (Illumina, Cat# FC‐131‐1024). The libraries were sequenced on the AVITI platform (Element Biosciences). Histology of mouse organs Histology

(12) A human-associated Spiroplasma ixodetis lineage responsible for infantile cataracts and adult febrile illnessiScienceApril 17, 2026Marie Buysse, Matthew J. Ballinger, Marjorie Bruley, Julien Amoros, Justine Grillet, Navid Farassat, Annerose Serr, Wolf Alexander Lagrèze, Christine Wennerås, Anna Grankvist, et al.assays ,, DNeasy Blood and Tissue Kit,Quiagen,Cat#69504 Nextera XT DNA Library Preparation Kit,Illumina,Cat#FC-131-1024 ,, Deposited data,Deposited data,Deposited data ,, Spiroplasma multi-locus sequences,This study,"NCBI

(13) Novel GH115 xylan α-1,2-glucuronidases with distinct preferences for xylan-derived oligomers and polymersProtein SciApril 8, 2026Savvina Leontakianakou, Simone Balzer Le, Anders Sundin, Andrius Jasilionis, Giang-Son Nguyen, Anna Nordborg, Lalitha D. Gottumukkala, Carl Grey, Anna Sofia Lewin, Eva Nordberg Karlssonsequencing libraries were made following the Nextera XT DNA Library Preparation Kit (Illumina cat. FC131‐1024) and sequenced by the Illumina MiSeq instrument. The generated sequence dataset was stored locally on

(14) A single-cell and spatial atlas of prostate cancer reveals the combinatorial nature of gene modules underlying lineage plasticity and metastasisbioRxivMarch 31, 2026Hanbing Song, Junxiang Xu, Keila Velazquez-Arcelay, Arda Demirci, Brendan L. Raizenne, Sarah C. Hsu, Joshua Choi, Julia H. Pham, Yih-An Chen, Hannah N. W. Weinstein, et al.KAPA HiFi HotStart ReadyMix PCR Kit,Kapa Biosystems,KK2602 Nextera XT DNA Library Preparation Kit,Illumina,FC-131-1024 10x Genomics Chromium Single Cell 3’ Kit,10x Genomics,PN-1000691 Xenium Prime 5K Human Pan Tissue

(15) Rapid Sequence Identification of Foot-and-Mouth Disease Virus Utilizing FMDV-ONTAPS: The Oxford Nanopore Technologies Amplicon P1 Sequencing ProtocolVirusesMarch 28, 2026Sean Yeo, Kate Hole, Taeyo Chestley, Grace E. Seo, Anna Majer, Katherine Handel, Michelle Nebroski, Oliver Lung, Charles Nfon, Shawn Babiukwith nuclease-free water. Libraries were prepared using the Nextera XT DNA Library Preparation Kit (FC-131-1024, Illumina, San Diego, CA, USA) and Nextera XT Index Kit v2 (FC-131-2001, Illumina) following the manufacturer’s

(16) A fine kinetic balance of interactions directs transcription factor hubs to genesbioRxivMarch 20, 2026Samantha Fallacaro, Apratim Mukherjee, Puttachai Ratchasanmuang, Joseph Zinski, Yara I Haloush, Kareena Shankta, Mustafa Mirassay,Agilent,5067–5579, Phasemaker™ tubes,ThermoFisher Scientific,A33248, Nextera XT DNA Library Preparation Kit,Illumina,FC-131–1024, Illumina Tagment DNA Enzyme and Buffer Small Kit,Illumina,20034197, Nextera DNA CD Indexes 24 indexes

(17) Chromatin reorganization drives overexpression of a Btaf1 variant underpinning hematopoietic agingNature CommunicationsMarch 18, 2026Le Zong, Bongsoo Park, Yaqiang Cao, Fei Ma, Ferda Tekin-Turhan, Wakako Kuribayashi, Keji Zhao, Isabel Beermanmanufacturer’s protocol. Sequencing libraries were constructed using Nextera XT DNA Library Preparation Kit (FC-131-1024, Illumina) with 125 pg input cDNA. Sequencing was done with the Illumina HiSeq 2500 instrument for WT

(18) Heme enhances B-cell proliferation and plasma cell formation through reduced p21 and Rb expressionJ ImmunolMarch 18, 2026Herbey O Padilla-Quirarte, Anna K Kania, Nicolas V Janto, Bagdeser Akdogan-Ozdilek, Sakeenah L Hicks, Carly J Roman, Mansi Gupta, Jeremy M Boss, Christopher D Scharerkit. Final libraries were generated using 200 pg of cDNA as input for the NexteraXT kit (Illumina, FC-131-1024) with 12 cycles of PCR amplification. Final RNA sequencing (RNA-seq) libraries were quantitated by QuBit

(19) Multiplexed optical barcoding and sequencing for spatial omicsScientific ReportsMarch 18, 2026Aditya Venkatramani, Didar Ciftci, Khanh Pham, Limor Cohen, Leonardo Sepulveda, Christopher Li, Xiaowei ZhuangAmpure XP beads at a 0.8x v/v ratio. We used the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024) for tagmentation with a few modifications. First, we amplified the tagmented products with our custom

(20) Strain variation identifies a neural substrate for behavioral evolution in DrosophilaCurrent BiologyMarch 9, 2026Anna Ryba, Philipp Brand, Rory T. Coleman, Yarden Greenfeld, Yael N. Tsitohay, Florian Hollunder, Katharine R. Keller, Murtaza Hathiyari, Tianyi Wei, Paolo Emilio Barbano, et al.PolyA selection and library preparation using the Nextera XT DNA library preparation kit (Illumina #FC-131-1024). Samples (3 each for CO13N, CS, and hybrid) were pooled before distributing the pool across sequencing

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Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina

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Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina

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