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Assay Kit
Nextera XT DNA Library Preparation Kit (24 Samples)
Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina
Be the first to write a review!
Citations:
(551)
Supplier Page
Supplier Page from
Illumina for
Nextera XT DNA Library Preparation Kit (24 Samples)
Description
90-minute tagmentation-based library prep for small genomes, PCR amplicons, plasmids, and cDNA with low DNA input requirement
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Nextera XT DNA Library Preparation Kit (24 Samples)
Company
Illumina
Price
Supplier Page
View Company Product Page
Catalog Number
FC-131-1024
Applications
Amplicon sequencing, WGS, De novo sequencing, Metagenomics
Reactivity
Any
Type
DNA Library Prep Kit
Quantity
24 Samples
Add to Compare List
Illumina
5200 Illumina Way
San Diego
,
CA
92122
United States
Phone
: 858.202.4500
Company Profile
Website:
http://illumina.com
Citations (551)
(1)
Axonal defasciculation is restricted to specific branching points during regeneration of the lateral line nerve in zebrafish
Development
August 15, 2026
Rohan S. Roy, A. J. Hudspeth
of cDNA was then used to prepare libraries using the Illumina Nextera XT DNA sample preparation kit (
FC-131-1024
,
Illumina
). Libraries generated from hair cells 1 day post nerve lesion and control hair cell samples
(2)
Dose-dependent supplementation of Schizochytrium in the biofloc system modulates dual microbiomes to enhance growth and survival in Pacific white shrimp (Litopenaeus vannamei)
Front Microbiol
July 22, 2026
Zhi Li, Xiaohan Li, Buwen Sun, Hongwei Wu, Jingyi Zhang, Xiulin Wan, Baohua Zhao, Nasi Xiao, Zhen Qi, Qingyang Li,
et al.
concentrations, and approximately 5 μg
sequencing
libraries were created using a TruSeq DNA Sample Prep Kit (
FC-131-1024
,
Illumina
, United States) before being sequenced on an Illumina PE300 platform (Illumina, United States). Raw
(3)
NodoMap: A single-cell and spatial transcriptomic atlas of the mouse nodose ganglion
Cell Press Blue
July 20, 2026
Sijing Cheng, Georgina K.C. Dowsett, Kara Rainbow, Mariana Norton, Anna G. Roberts, Phyllis Phuah, Gavin A. Bewick, Brian Y.H. Lam, Giles S.H. Yeo, Kevin G. Murphy
kit,Curio Bioscience,"SQ8002, K001, K002, K003" Nextera XT
DNA Library Preparation
Kit,"
Illumina
, USA",
FC-131-1024
,, Deposited data,Deposited data,Deposited data ,, NodoMap - snRNASeq of
mouse
nodose
ganglion
,This
(4)
Gut commensal bifidobacteria are associated with restrained inflammatory reprogramming in human monocyte-derived dendritic cells
iScience
July 17, 2026
Sayaka Ishihara, Tatsuki Nishimura, Riko Mishima, Eri Mitsuyama, Akira Sen, Hiroki Kaneko, Yukihiro Hishida, Miyuki Tanaka, Toshitaka Odamaki
RAW2,Macherey-Nagel,Cat#740359.80 SMART-Seq mRNA LP,Takara Bio,Cat#634768 Nextera XT
DNA Library Prep
Kit,
Illumina
,Cat#
FC-131-1024
"IDT for Illumina - DNA/RNA UD Indexes, Tagmentation",
Illumina
,Cat#20091654
NovaSeq X
Series 25B Reagent
(5)
Decoding stage-specific functions of PRC2 in early embryogenesis uncovers roles in preimplantation development and primordial germ cell fate
Nat Cell Biol
July 10, 2026
Chengjie Zhou, Meng Wang, Zhiyuan Chen, Yi Zhang
634890) was used to construct cDNA libraries, and Nextera® XT DNA Sample Preparation Kit kit (
Illumina
,
FC-131–1024
) was used for the sequence libraries construction. All libraries were sequenced by the Illumina NextSeq
(6)
Cerebellar microRNA-206 tunes Purkinje neuron firing dynamics to control sensorimotor gating
bioRxiv
June 30, 2026
Mary P. Heyer, Masago Ishikawa, Junshi Wang, John Erol Evangelista, Amanda K. Fakira, Avi Ma'ayan, Guoping Feng, Paul J. Kenny
Illumina,
New England Biolabs
,Cat#
E7560S
Nextera XT DNA Sample Preparation Kit for 24 Samples,
Illumina
,Cat#
FC-131-1024
Curio Seeker 3×3 Seeker Kit Bundle V1.1 (8 tiles),Curio Biosciences,Cat#SK004 Curio Seeker Dual Indexing
(7)
Epidemiological and Epizootological Monitoring and Spatiotemporal Dynamics of Plague in Natural Foci of Kazakhstan
Pathogens
June 29, 2026
Ziyat Abdel, Zauresh Zhumadilova, Raikhan Mussagalieva, Aigul Abdirassilova, Svetlana Issaeva, Galina Kovaleva, Bolatbek Baitursyn, Beck Abdeliyev, Temirkhan Sagidulin, Nurbol Shaki,
et al.
MD, USA). DNA libraries were prepared using the Nextera XT
DNA Library Preparation
Kit (catalog No.
FC-131-1024
) according to the manufacturer’s protocol.
Sequencing
was performed on the
Illumina
MiSeq platform using
(8)
Local and systemic immune correlates of anal high-risk HPV infection and clearance in men living with HIV and men at high risk for HIV
Frontiers in Microbiology
June 25, 2026
Veronica Ober, Eva Gruener, Danni Wang, Olga Baranov, Renate Stirner, Gabriele Reiling, Raffaele Conca, Christopher Dächert, Gerardo Ibarra, Ulrich Seybold,
et al.
(
Roche
; #7958935001)]. Libraries were generated with Nextera XT
DNA Library Preparation
Kit (
Illumina
; #
FC-131-1024
) and Nextera XT Index Kit v2 Set (Illumina; FC-131-2001) according to manufacturer’s protocol but using
(9)
Linking Composition and Function: A Two-Year Metatranscriptomic Study of Microbial Adaptation in an Industrial Wastewater Treatment Plant
FEMS Microbiology Ecology
June 18, 2026
Asala Mahajna, Ranko Gacesa, Karel J Keesman, Gert-Jan W Euverink, Andreas Froemelt, Carlos J Melián, Bayu Jayawardhana
subsequently prepared using the Nextera XT DNA Sample Preparation Kit (
Illumina
, San Diego, CA, USA; Cat. No.
FC-131-1024
), and
sequencing
was performed on the MiSeq platform at the University Medical Center Groningen
Sequencing
(10)
Differentiation defects reposition sebaceous glands as inflammatory instigators in the early pathogenesis of hidradenitis suppurativa
Front Immunol
June 8, 2026
Xiang Chen, Jiaqi Li, Yibo Feng, Zhanyan Pan, Qiong Wu, Xinyi Xu, Qian Zhao, Tingting Hu, Guangjie Chen, Christos C. Zouboulis,
et al.
transcription of RNA were processed. The cDNA was amplified with the Nextera XT DNA sample Pre-Kit (
FC-131-1024
,
Illumina
, San Diego, USA) to gain
sequencing
libraries. Libraries of individual samples were measured
(11)
Clinical severity-dependent virulence of Staphylococcus aureus from human diabetic foot ulcers drives impaired wound healing in a diabetic murine model
Front Cell Infect Microbiol
June 5, 2026
Keun Seok Seo, Lee M. Nicols, Cara Dale Palmer, K. Taylor Hellmann, D. Ashley Robinson, Alicia K. Olivier, Justin A. Thornton, Khadija Ferdous, Wilson Cooper Brookshire, Joo Youn Park
Scientific, USA), and 150bp paired-end libraries were prepared with a Nextera XT
DNA Library Preparation
Kit (
FC-131-1024
,
Illumina
, USA).
Sequencing
was performed at the Molecular and Genomics Core Facility of the University
(12)
Commensal-derived acetylcholine enhances mucosal immune education
Nature
June 3, 2026
Deguang Song, Brianna Duncan-Lowey, Varnica Khetrapal, Randy Hamchand, Tong Deng, Hailey Brown, Anchi Wu, Anjelica L. Martin, Kaylyn M. Bauer, Yanyu Zhao,
et al.
Sequencing
libraries were prepared using the Illumina Nextera XT library preparation kit (
Illumina
,
FC-131-1024
) and barcoded using the Nextera XT Index Kit V2 (Illumina, FC−131-2001). The libraries were sequenced
(13)
Squamous-state excursions activate APOBEC3A in cancer
bioRxiv
May 28, 2026
Josefine Striepen, Alexandra Dananberg, Aušrinė Ruzgaitė, Alyssa Hurley, Eléonore Toufektchan, Ashley Nichols, Hannah Rosenberg, Cameron Cordero, Tony M. Mertz, Roshan Xavier Norman,
et al.
ng of cDNA was used to prepare libraries with the Nextera XT
DNA Library Preparation
Kit (
Illumina
,
FC-131–1024
) in a total volume of 6.25 μL with 8–12 cycles of
PCR
. Indexed libraries were pooled by volume and cleaned
(14)
COX2 + / PDGFRα + fibroblasts selectively localize near bile ducts and interact with immune cells in early liver fibrosis in mice
Physiological Reports
May 24, 2026
Momo Goto, Tamaki Kurosawa, Hiroyuki Koike, Noriyuki Kaji, Ichiro Manabe, Taiki Mihara, Takashi Chaen, Yumiko Oishi, Masatoshi Hori
Sequencing
(# 634888, Takara Bio, Shiga, Japan) and Nextera TX
DNA Library Prep
Kit for Illumina (#
FC‐131‐1024
,
Illumina
, San Diego, CA, USA). Libraries were sequenced using Illumina NovaSeq 6000 (Illumina), and
(15)
Age-related decline in nuclear envelope LINC complex drives neuronal aging via axon initial segment dysfunction
EMBO Reports
May 22, 2026
Koichi Hasegawa, Noriyuki Hama, Mina Amemiya, Chao Zeng, Yasuyuki Ito, Sadafumi Suzuki, Keiichiro Nakamura, Junpei Kondo, Chiharu Takeda, Yuji Kurihara,
et al.
A510008 SMART-Seq mRNA Kit,Takara Bio,Cat. #: 634772 Nextera XT
DNA Library Preparation
Kit,
Illumina
,Cat. #:
FC-131-1024
AAV-Venus,This paper,N/A AAV-LINC-DN,This paper,N/A AAV-Sun1-3xHA,This paper,N/A AAV-eGFP-Cre,This
(16)
UMI-guided single locus sequence typing method for phylotyping Cutibacterium acnes from skin samples
Frontiers in Cellular and Infection Microbiology
May 20, 2026
Elody Orcel, Erwin Sentausa, Hayat Hage, Karen Louis, May Taha, Samuel Bellais, Adrien Villain, Laurent Beloeil, Steven Sijmons, Nathalie Devos,
et al.
subjected to
Illumina library preparation
using the Nextera XT
DNA Library Preparation
Kit (
Illumina
, #
FC-131-1024
) with minor modifications. Briefly, 5 µL of pooled DNA diluted to 0.2 ng/µL were mixed with 10 µL of
(17)
Endocrine therapy reprogramming of breast cancer facilitates metastatic escape via upregulation of P-Rex1/Rac1 signalling
Nat Commun
May 11, 2026
Kristine J. Fernandez, Ghazal Sultani, Max Nobis, Brian Gloss, Leila Eshraghi, Amy E. McCart Reed, Sarah Alexandrou, Christine Lee, Daniel L. Roden, Emily I. Jones,
et al.
amplified by
PCR
. Pooled cDNAs were then tagmented with the Nextera XT DNA Library kit (
Illumina
Cat#
FC-131-1024
) and custom primers as in ref. 96. Tagmented and multiplexed cDNA libraries were sequenced as in ref.
(18)
HIV-driven virome dysbiosis unveils distinct virome features and inter-viral correlations in blood and respiratory niches
Communications Biology
May 8, 2026
Wang Li, Ping Ni, Juan Xu, Xingyue Zhao, Anhua Dou, Yanhuan Wang, Linjie Peng, Shiyin Huang, Yue Chen, Qi Shi,
et al.
polymerase (
NEB
,
M0210L
), 1 μl/pool, followed by library preparation with Nextera XT Pre kit (
Illumina
,
FC-131-1024
) and paired-end
sequencing
(MiSeq, 2 × 250 bp). The
sequencing
quality statistics for each sample are
(19)
RNase P/MRP subunits chaperone telomerase holoenzyme assembly in fission yeast
EMBO Reports
April 28, 2026
Lili Pan, Valentine Patterson, Martin M Möckel, Rachel M Helston, Raymund J Wellinger, David C Zappulla, Peter Baumann
equimolar ratios, and dual-indexed libraries were prepared using the Nextera XT
Library Prep
Kit (
Illumina
,
FC-131-1024
). Libraries were sequenced as 2×150 bp paired-end reads on a NextSeq 500 or 2000 platform. SHAPE data
(20)
Two molecularly-defined neuronal types in the mammillary body govern different temporal periods during working memory maintenance
Research (Wash D C)
April 22, 2026
Xinyan Guo, Lanfang Guo, Lanfang Hou, Zhenye Hou, Xiaomei Tang, Xiaomei Tang, Changdong Zeng, Changdong Jiang, Fuliang Jiang, Zhigao Xiang,
et al.
(
Agilent
5067-4626
).
Sequencing
libraries were prepared with Nextera XT
DNA Library Prep
Kit (
Illumina
FC-131-1024
), followed by
sequencing
on Illumina NovaSeq (>1 million reads per sample). TopHat2 (v2.1.0) aligned
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Nextera XT DNA Library Preparation Kit (24 Samples) from Illumina
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