Description
Europium cryptate labeling kit tailored for biomolecule labeling and straightforward development of time-resolved fluorescence resonance energy transfer assays. The Cryptate labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with Europium cryptate. This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRFassay development.The N-hydroxysuccinimide activated Europium-Trisbipyridine (TBP) supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).HTRF assays offer many advantages over other technologies:
Homogeneous add-and-read format
No wash steps
Low background
Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
Stable signal, providing flexibility in time of readout or size of assays