Description
Principle of the Assay::The Human CD93 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD93 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD93 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD93 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD93 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD93 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo