Description
Background: Matrilin-2 is a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues.The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.MATN2 contains 2 VWFA-like domains, 10 EGF-type modules, and an oligomerization module. RT-PCR demonstrated wide expression of MATN2 in human cell lines of fibroblastic or epithelial origin. Two mRNA transcripts were detected which differ from each other in a short region that is unique to matrilin-2; 19 triplets are included or spliced out of the mature mRNA as a result of alternative 3-prime splice site selection.
Principle of the Assay: Human Matrilin-2 (MATN2) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN2 in samples. An antibody specific for MATN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN2 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MATN2 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MATN2 bound in the initial step. The color development is stopped and the intensity of the color is measured