Description
Principle of the Assay: This kit applies the indirect method (ELISA). The microtiter plate has been pre-coated with TNFalpha MCAB antigen (solid phase antigen), standards or samples are added to the microtiter plate wells, then add anti-human IgG HRP labeled antibody. Wells are thoroughly washed to remove all unbound components. And a sandwich complex of solid antigen-antibody enzyme-labeled secondary antibodies is formed on the solid surface of the microplate. Next, Substrate solution A and substrate solution B are added to each well, and it will produce blue materials under HRP catalysis. The enzyme-substrate reaction is terminated by the addition of sulphuric acid and the color change to yellow. Measured spectrophotometrically at a wavelength of 450 nm, the absorbance (OD value) is positively correlated with the concentration of TNFalpha MCAB in the sample. The concentration of TNFalpha MCAB in the samples could be determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This kit is used for detecting the TNFalpha MCAB concentration of serum, plasma, and cell culture supernatant