Description
Background: Matrin 3(MATR3) is localized in the nuclear matrix. It may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Two transcript variants encoding the same protein have been identified for this gene.The deduced 845-amino acid protein has a calculated molecular mass of approximately 95 kD. Its primary structure consists of 33% charged residues and is generally hydrophilic. Like the lamins, matrin-3 has a positively charged N terminus that contains a large number of amino acids with free hydroxyl groups. A highly acidic domain near the C terminus, in which 32% of the amino acids are acidic, is a characteristic found in other nuclear proteins.
Principle of the Assay: Human Matrilin-3 (MATN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN3 in samples. An antibody specific for MATN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MATN3 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human MATN3 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of MATN3 bound in the initial step. The color development is stopped and the intensity of the color is measured