Description
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDNF in human serum, plasma, cell culture supernatants and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human GDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDNF present is bound by the immobilized antibody. After washing away any unbound substances, and then a detection antibody specific for GDNF is added to the wells and binds to the combination of capture antibody GDNF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of GDNF bound in the initial step. The color development is stopped and the absorbance is measured