Description
The Human Estradiol E2 ELISA Kit is a solid-phase sandwich Enzyme‑Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of 17-beta-estradiol (E2) in serum and plasma. The assay will recognize both natural and recombinant human estradiol E2.
Principle of the method
The Human Estradiol solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation