Description
Whole transcriptome library prep that generates high-quality libraries in under 4.5 hours. A highly streamlined Polaris Depletion module depletes highly abundant rRNA and globin transcripts in human, mouse, and rat samples, providing improved coverage of biologically interesting transcripts, including long non-coding RNAs. The automation-friendly workflow reduces total turnaround time, hands-on time, and consumable use through a reduction in bead purification and reaction steps. A novel, engineered reverse transcriptase improves the conversion of RNA to cDNA, enabling high-quality performance with 1 ng to 1 µg of total RNA, as well as FFPE-derived RNA.
- Increase sensitivity and retain quantitative gene expression information with low-input samples, down to 1 ng
- Access more biologically relevant information from FFPE samples
- Generate stranded libraries in under 4.5 hours, including rRNA and globin depletion
- Improve automatability with fewer purifications and reagent additions
- Effectively sequence blood samples while covering lncRNAs