Description
This experiment aims to count cells that secrete human interleukin-3 (IL-3). The kit includes monoclonal antibodies, biotinylated detection antibodies, Streptavidin-ALP and ELISpot plates pre-coated with BCIP/NBT-plus substrates. Human T cell polyclonal activator (mAb CD3-2) was used as a positive control for cytokine secretion. The pre-coated plate reduces the measurement time and minimizes variability