Description
This kit has been developed to isolate CD24-CD44+ breast cancer stem cells by negative selection and positive selection. Unwanted cells are depleted by labeling biotin conjugated anti-CD24 antibody followed by incubation with magnetic streptavidin nanoparticles, leaving CD24- cells in suspension. Then cells in suspension are labeled with biotinylated anti-CD44 antibody that targets the desired cells. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, mmune statusmonitoring, or other functional assays.