Cell Counting Kit-8 (CCK-8) C0005 from TargetMol

Product Specs
ItemCell Counting Kit-8 (CCK-8)
CompanyTargetMol
Price $79.00Supplier Page View Company Product Page
Catalog NumberC0005
TypeChemical
Quantity10 mL (1000 tests)

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TargetMol

34 Washington Street, Suite 220

Wellesley Hills, MA 02481

United States

Phone: +1-781-999-5354

Email: sales@targetmol.com

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Website: www.targetmol.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangcell-count validation during counting. Cell viability was assessed via Cell Counting Kit-8 (CCK-8) assay (#C0005, TargetMol). At each time point (0, 24, 48, 72, and 96 h), 10 μL of CCK-8 reagent was added to each

(2) Physical activity-associated extracellular vesicles inhibit inflammagen-induced microglial activationBrain, Behavior, & Immunity - HealthMay 1, 2026Chien-Yu Su, Heng-Juei Hsu, Tzu-Feng Wang, Hong-Lin Chou, Sheng-Feng Tsai, Chu-Wan Lee, Ching-Li Cheng, Yang-Kao Wang, Yu-Min Kuodescribed in the experimental design. One hour before the end of treatment, 22 μL of CCK-8 reagent (Cat#: C0005, TargetMol, Boston, MA, USA) was added to each well and incubated for 1 h at 37 °C. Absorbance at 450

(3) ATF3/SLC31A1-Mediated Cuproptosis Contributes to Bortezomib-Induced Peripheral Neurotoxicity and Intervention by (−)-Epigallocatechin GallateInt J Mol SciApril 21, 2026Yonghai Wang, Jiabin Lu, Xuejing Feng, Bo Yang, Qiaojun He, Peihua Luo, Xiaochun Yangidentifier PXD072911. 4.5. CCK-8 The cell inhibition rate was assessed using the CCK-8 (Catalog No. C0005, TargetMol). Following the removal of the existing culture medium, fresh medium containing 10% CCK-8

(4) Modulation of Iron-Induced Glucose Metabolic Reprogramming Alleviates Retinal Pigment Epithelial Cell SenescenceInvest Ophthalmol Vis SciApril 14, 2026Zhenzhen Zhao, Qingjian Ou, Jincheng Zhu, Zixu He, Yike Yang, Jiaming Jiang, Qian Wang, Ye Zhou, Ying Liu, Xiaoman Zhu, et al.statistical analysis. Cell Viability Assay Cell viability was determined using a Cell Counting Kit-8 (C0005; TargetMol Chemicals). The iRPE cells were seeded in 96-well plates at 1.0 × 104 cells per well and

(5) Erianin facilitates pyroptosis in endometrial cancer via targeting m6A reader YTHDF1Chin MedApril 7, 2026Wan Shu, Xing Zhou, Rong Zhao, Kejun Dong, Xiaoyu Shen, Guanxiao Chen, Shuangshuang Cheng, Qi Zhang, Ting Zhou, Jiarui Zhang, et al.(Houston, TX, USA). Cell viability assay Cell viability was assayed using Cell Counting Kit-8 (CCK-8; C0005; TargetMol, Boston, USA). An amount of 5 × 103 cells/well were seeded into 96-well plates and incubated

(6) Pterostilbene attenuates lung ischemia-reperfusion injury: integrative insights from network pharmacology, molecular dynamics, and experimental validationFront PharmacolApril 1, 2026Haotian Bai, Heng Zhao, Jinteng Feng, Hongyi Wang, Yixing Li, Zhe Chen, Bin He, Chi Wang, Rui Gao, Rui Zhao, et al.Cell counting Kit-8 (CCK-8) assay Cell viability was evaluated with the Cell Counting Kit-8 (CCK-8, C0005, TargetMol, United States) according to the guidelines of the manufacturer, employing a colorimetric

(7) Arginine dependency in omental metastasis of epithelial ovarian cancer reveals a therapeutic vulnerabilityCell Death & DiseaseMarch 24, 2026Jiming Tian, Ting Lei, Yulu Du, Dalin Wang, Haiping Liang, Zeyu Yan, Jun Xu, Wenhao Xue, Shuangbin Wang, Xuehan Bi, et al.48, 72, or 96 h) at 37 °C under 5% CO₂. To assess cell viability, 10 μL of CCK-8 reagent (Cat. No. C0005, TargetMol, Shanghai, China) was added to each well and incubated for 3 h. Absorbance was measured at

(8) Hypoxia-Induced GRP78 Activation Disrupts the Fndc5/Irisin Axis to Accelerate Skeletal Muscle AtrophyCell Stress and ChaperonesMarch 23, 2026Shiqiang Liu, Linyao Xu, Xinru Song, Zhenhao Zhu, Yuxin Zhang, Yumeng Sun, Sharon Nyoja, Qin Wang, Jialin Gao, Lizhuo Wang, et al.HA15 on cell viability was assessed using the Cell Counting Kit-8 (CCK-8; TargetMol, Boston, MA, USA; C0005). Initially, 2.5 × 10⁴ C2C12 murine myoblasts were seeded into a 96-well plate. Upon reaching 70%-80%

(9) Fisetin alleviates lipopolysaccharide-induced mastitis by inhibiting ferroptosis and modulating the gut microbiotaVet QMarch 17, 2026Chao Tong, Li Wang, Haojie Wen, Xinhui Yao, Jiang Tong, Ruya Zhang, Xiao Li, Yuqiang Xiang, Xuebing Wangpurchased from Solaibro. The Cell Counting Kit-8 (CCK-8) was purchased from TargetMol Chemicals Inc. (C0005), while Trizol (R401-01), HiScript II Q RT SuperMix for qPCR (+gDNA wiper) (R223-01), and ChamQ Universal SYBR qPCR Master Mix (Q711

(10) Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopeniaJournal of NanobiotechnologyMarch 16, 2026Benchi Che, Yongzhi Cui, Zhengsheng Chen, Yanchun Gao, Lei Luo, Kaiwen zheng, Jiashuo Liu, Yu Xiang, Jiaqi Cheng, Yuanyuan Guo, et al.in vitro Mouse BMSCs and MuSCs were used to access the cytotoxicity of miR@DT/iMNV with a CCK-8 kit (C0005, TargetMol, Boston, MA, USA). Different cells were incubated with PBS/iMSC-EV/miR@DT-Lipo/ miR@DT/iMNV

(11) An integrative analysis reveals the mechanism of plastic stabilizers inducing breast cancerPLoS Comput BiolMarch 6, 2026Xingfa Huo, Xueqin Duan, Xiaojuan Huang, Linyuan Xue, Lantao Zhao, Yufeng Li, Xiaochun Zhang, Na Zhoutime points, the original medium was replaced with a culture medium containing 10% CCK-8 reagent (Cat#C0005, TargetMol, USA). Following a 2-hour incubation period in a humidified incubator at 37°C, the absorbance

(12) Engineering NIR-II carbon dots through aniline extension with graphene and nitrogen enrichment for hepatobiliary theranosticsNature CommunicationsMarch 2, 2026Lijuan Yang, Man Li, Yisheng Peng, Yu Zhou, Junjie Zou, Huanhuan Liu, Wenxiang Zhang, Jie Liu, Peng He, Fan Dai, et al.sterile PBS and treated with 100 μL of fresh culture medium and 10 μL of CCK-8 solution (TargetMol, C0005, USA) for an additional 2 h at 37 °C. Cell viability was assessed by measuring the absorbance at 450

(13) Bifendate inhibits cell PARthanatos by activating the MEK/ERK pathwayBiochemistry and Biophysics ReportsMarch 1, 2026Ao-meng Hu, Meng Chen, Can-can Zhu, Zai-man Zhu, Xiao-hui Xua density of 1.3 × 104 cells/well. After the indicated treatments, 10 μL CCK-8 reagent (TargetMol, #C0005) was added to each well, and incubated for 2 h at 37 °C. The absorbance was then measured at 450 nm,

(14) Mechanistic evidence for dibutyl phthalate as an environmental trigger for inflammatory bowel diseaseiScienceFebruary 20, 2026Hang Yuan, Gang Chen, Xuejun Sun, Junhui Yuindicating optimal affinity. CCK-8 Cell viability was assessed using the Cell Counting Kit-8 (CCK-8; CAT#C0005, TargetMol, USA). Initially, NCM460 cells were plated into 96-well plates at a density of 4,000 cells

(15) Cigarette Smoke Extract Combined with LPS Upregulates PITPβ Expression in Chronic Pulmonary Inflammation and May Be Related to the EGFR/ERK Signaling PathwayToxicsFebruary 18, 2026Yan Sun, Haojie Li, Xueqing Zhu, Jue Songserum albumin; BS114-25) was purchased from Biosharp (Beijing, China). Cell counting kit-8 (CCK8) (C0005) was purchased from TargetMol (Shanghai, China). 2.2. Preparation of Rat Models The experiment employed

(16) A conserved eIF1A+ luminal cell-centered hypoxic and “cold” tumor microenvironment promotes pan-subtype prostate cancer progressionCell Reports MedicineFebruary 17, 2026Yifei Cheng, Lilin Wan, Enyao Huang, Bing Zheng, Xuefei Ding, Song Tan, Gaoxiang Ma, Wenchao Li, Chunyan Chu, Tiange Wu, et al.IHC kit for mouse,Absin,Cat# abs50030 CoCl2,Macklin,Cat# C885204 Cell Counting Kit-8,TargetMol,Cat# C0005 Degarelix Acetate,TargetMol,Cat# 934016-19-0 Homoharringtonine,TargetMol,Cat# 26833-87-4 ,, Experimental models

(17) Targeting NPY5R—A Member of the NPY Receptor Family: Pharmacological and Transcriptomic Mechanisms of the Euphorbia Factor L2 Against Lung AdenocarcinomaPharmaceuticals (Basel)February 15, 2026Pengzhuo Tao, Wei Liu, Yongfu Wang, Yajing Xue, Changmin Liu, Yizhen Yuan, Kim Fey Leu, Shilin Chen, Chi SongFollowing treatment, 100 µL of CCK-8 working solution, freshly prepared by mixing 10 µL of CCK-8 reagent (C0005; TargetMol, Shanghai, China) with 90 µL of culture medium, was added to each well, and the plates were

(18) KIF20A inhibits TRIM21-dependent ubiquitination of DHX9 to boost SOX2 stability, enhancing OSCC stemness and ferroptosis resistanceCell Death & DiseaseFebruary 11, 2026Ziyun Zhang, Yi Li, Jingjiang Hu, Xingjie Tang, Zhanpeng Li, Xiaoyan Sun, Dade Feng, Yixin Yao, Chao Mao, Yongguang Tao, et al.96-well plates for indicated days, cell proliferation was assessed using a Cell Counting Kit-8 (CCK-8, #C0005, TargetMol). The CCK-8 solution was diluted 1:10 in medium and incubated with the cells at 37 °C for

(19) Lactylation‑based machine algorithm combined with multi‑omics analysis to predict prognosis in cervical cancerOncology LettersFebruary 11, 2026Ruyue Wang, Li Ning, Xiu Li, Yating Xu, Yu Si, Hongting Zhao, Qingling RenHealth). Cell Counting Kit-8 (CCK-8) assay Cell proliferation was assessed using the CCK-8 Assay Kit (cat. no. C0005; TargetMol Chemicals Inc.). Cells were seeded in 96-well plates and absorbance was measured at 24, 48

(20) Curcumin pretreatment enhances the capacity of BMSC exosomes to attenuate renal ischemia-reperfusion injury by ferroptosis suppression via miR-16-5p/Smad3/Mb axisStem Cell Research & TherapyFebruary 9, 2026Bingyi Chen, Jinxing Chen, Zekun Shen, Shuang Liu, Yifan Mei, Hui Cai, Kai Li, Ziyang Peng, Liwei Zhang, Weiyi Wang, et al.medium, followed by an additional 24 h culture. Under light-protected conditions, 10 µL CCK8 solution (#C0005, TargetMol) was added to each well. The plate was returned to the incubator for 30 min incubation before

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Cell Counting Kit-8 (CCK-8) from TargetMol

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Cell Counting Kit-8 (CCK-8) from TargetMol

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