NEBNext UltraExpress® RNA Library Prep Kit E3330L / E3330S from New England Biolabs

Product Specs
ItemNEBNext UltraExpress^® RNA Library Prep Kit
CompanyNew England Biolabs
Price Inquire
Catalog NumberE3330L / E3330S
Quantity96 reactions / 24 reactions
ApplicationsLibrary Construction, Next Gen Sequencing (NGS)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male developmentNat CommunApril 9, 2026Elisheva Abberbock, Meshi Ridnik, Isabelle Stévant, Roni Weiss, Carmel Bamberger, Shelly Ziv Lhermann, Maor Lubman, Yumi Minyi Yao, Ariel Afek, Francis Poulat, et al.Magnetic Isolation Module (NEB, E7490S), along with NEBNext UltraExpress RNA library prep kit (NEB, E3330S) and cleaned using AMPure beads (Beckman Coulter, Bc-a63881) according to manufacturer's instructions.

(2) Protective Effects of Cyclosporin H Against Sepsis-Induced Acute Kidney Injury via Modulation of Fpr1 Signaling and Inhibiting PyroptosisRes Rep UrolMarch 7, 2026Hangwei Feng, Linqian Jiang, Cuilian Wengintegrity. Libraries were constructed using Illumina’s NEBNext® UltraTM RNA Library Prep Kit (NEB, E3330S) and sequenced on the Illumina platform to generate 150 bp paired-end reads. The paired-end sequences

(3) A Cross-Species Hydroquinone Model Replicates Smoking-Related Corneal Endothelial Oxidative Injury and Enables Therapeutic Screening of FGF10Invest Ophthalmol Vis SciMarch 6, 2026Bryanna J. Lee, Devansh Agarwal, John H. Huang, Anastasiia Ivanova, Jennifer J. Bu, Christian A. Hill, Takako Noguchi, Jong Hwa Jun, Helen P. Makarenkova, Karl J. Wahlin, et al.prepared with the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA; cat. E3330L) and sequenced on a NovaSeq X Plus (100-bp paired-end reads; Illumina, San Diego, CA, USA) at the UCSD

(4) Emerging role of KDM5C in X-linked intellectual disability based on human genetic data and zebrafish modelsFrontiers in Molecular NeuroscienceFebruary 10, 2026Baoqiong Liao, Meihuan Chen, Yun Huang, Mei Shuai, Liangpu Xu, Shuwen He, Hailong Huangagarose gel electrophoresis. Subsequently, Illumina's NEBNext® UltraTM RNA Library Construction Kit (NEB, E3330S) was used to construct an RNA library, which was sequenced on the Illumina platform to obtain a paired

(5) GJB2 c.109G > A mutation activating IFI27-mediated mitochondrial apoptosis pathway leading to hereditary non-syndromic hearing lossScientific ReportsJanuary 26, 2026Yao Chen, Peiran Zhao, Qingying Lin, Yinglin Zeng, Xiaolong Qiu, Ting Huang, Jianping Tang, Liangpu Xuintegrity. Libraries were constructed using Illumina’s NEBNext® UltraTM RNA Library Prep Kit (NEB, E3330S) and sequenced on the Illumina platform to generate 150 bp paired-end reads. The paired-end reads were

(6) The role and potential mechanism of PEST-containing nuclear protein (PCNP) in endothelial cell angiogenesis in vitroSci RepDecember 11, 2025Yuxi Du, Chaoyuan Xu, Yalong DangSequencing libraries were prepared using the NEBNext Ultra™ RNA Library Prep Kit for Illumina® (Cat. No. E3330S, NEB, USA) and sequenced on an Illumina NovaSeq 6000 platform (Megigene Biotechnology Co., Ltd., China).

(7) Small Extracellular Vesicles From Radioresistant H3K27M-Pediatric Diffuse Midline Glioma Cells Modulate Tumor Phenotypes and Radiation ResponseJ Extracell VesiclesOctober 30, 2025Viral D. Oza, Kenan A. Flores, Yelena Chernyavskaya, Majd A. Al-Hamaly, Caitlyn B. Smith, Ronald C. Bruntz, Jessica S. Blackburntranscripts. Library preparation followed the NEBNext Ultra II Express Library Prep Kit for Illumina (E3330S, New England Biolabs) protocol, which included mRNA fragmentation at 94°C for 15 min, first‐ and second‐strand cDNA synthesis

(8) Microphysiological Uremia Model Reveals Biophysical Potentiators of Vascular DysfunctionAdvanced ScienceSeptember 1, 2025Mitesh Rathod, Ryan N. Stack, Sarah E. Kubik, Stephanie A. Huang, Chloe P. Whitworth, Wen Y. Aw, Elizabeth L. Doherty, Sara M. Meehan, Prabir Roy‐Chaudhury, William J. PolacheckLibraries were prepared using 50 ng input RNA and the NEBNext ULTRAEPXRESS RNA Library Prep Kit (NEB, E3330S) according to manufacturer's instructions. Libraries were quantitated and assessed for fragment size

(9) Absolute quantification of mammalian microRNAs for therapeutic RNA cleavage and detargetingRNAAugust 1, 2025Carolyn Kraus, Jiayi Wang, Haiying Zheng, Jennifer Broderick, Nandagopal Ajaykumar, Mina Zamani, Mengqi Yang, Katharine Cecchini, Shun-qing Liang, Olena Kolumba, et al.depletion (Supplemental Table S3) was performed with NEBNext UltraExpress RNA Library Prep Kit (NEB, #E3330S), except that UMI-containing adaptors were used (Fu et al. 2018). For sequencing of polyadenylated RNAs

(10) Transmission of maternal oral pathobionts to the infant gut predisposes offspring to exacerbated enteritisCell ReportsJuly 22, 2025Masafumi Haraguchi, Yeji Kim, Natsuru Watanabe, Kyoko Yamazaki, Kira L. Newman, Merritt G. Gillilland, Yijie Guo, Daisuke Watanabe, Ingrid L. Bergin, Takehiro Suzuki, et al.NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490), NEBNext UltraExpress RNALibrary Prep Kit for Illumina (NEB E3330L), and NEBNext Multiplex Oligos for Illumina Unique dual (NEB E6440L). Total RNA was converted to mRNA

(11) Draft genome sequence of Modicisalibacter sp. strain Wilcox isolated from produced waterMicrobiology Resource AnnouncementsFebruary 11, 2025Damilare Ajagbe, William Marsh, Robert Murdoch, Babu Fathepurefragments of 350 bp, and the DNA library was constructed using the NEBNext Ultra DNA Library Prep Kit (E3330L), New England Biolabs, Massachusetts, USA, following the manufacturer’s protocol. Sequencing was performed

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NEBNext UltraExpress® RNA Library Prep Kit

NEBNext UltraExpress® RNA Library Prep Kit

NEBNext UltraExpress^® RNA Library Prep Kit from New England Biolabs

Description

New England Biolabs

Citations (11)

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NEBNext UltraExpress^® RNA Library Prep Kit from New England Biolabs

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NEBNext UltraExpress® RNA Library Prep Kit

NEBNext UltraExpress® RNA Library Prep Kit

NEBNext UltraExpress® RNA Library Prep Kit from New England Biolabs

Description

New England Biolabs

Citations (11)

Reviews

NEBNext UltraExpress® RNA Library Prep Kit from New England Biolabs

NEBNext UltraExpress^® RNA Library Prep Kit from New England Biolabs

NEBNext UltraExpress^® RNA Library Prep Kit from New England Biolabs