T7 mScript™ Standard mRNA Production System V2 C-MSC11610 from CELLSCRIPT

Product Specs
ItemT7 mScript™ Standard mRNA Production System V2
CompanyCELLSCRIPT
Price $356.00Supplier Page View Company Product Page
Catalog NumberC-MSC11610
Quantity10 reactions
ApplicationsmRNA synthesis, Cell and gene therapy, mRNA vaccine development, Canonical nucleotide transcription
TypeCanonical nucleotide transcription

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CELLSCRIPT

726 Post Road

Madison, WI 53713

United States

Phone: Toll-Free within the USA:
+1 888 826 5586

Tel: +1 608 442 6484

Email: customerservice@cellscript.com

Company Profile

Website: www.cellscript.com

(1) ESPEC-SUIT: a versatile and robust platform to identify and track antigen-specific T cell receptors in patients with cancerJournal for ImmunoTherapy of CancerSeptember 21, 2025Katharina Lindner, Saskia R Stange, Julian Benedikt Hlawatsch, Moritz Hamberger, Tamara Boschert, Chin Leng Tan, Ingrid Hülsmeyer, Samira Moreen Sachse, Simone Jünger, Alexander Ernst, et al.the resulting PCR product was used as a template for the T7-mScript Standard mRNA-Production-System (C-MSC100625, CELLSCRIPT). mRNA was m7G capped and enzymatically polyadenylated following the manufacturer’s instructions. TCR

(2) Adaptive evolution of CENP-T modulates centromere bindingCurrent BiologyMarch 10, 2025Damian Dudka, Alexandra L. Nguyen, Katelyn G. Boese, Océane Marescal, R. Brian Akins, Ben E. Black, Iain M. Cheeseman, Michael A. Lampsonng/μl; gRNA – 20-30ng/μl. mRNAs were synthesized using the T7 mScriptTM Standard mRNA Production System (C-MSC100625; CELLSCRIPT), purified using MEGAclear Transcription Clean-Up Kit (Thermo Fisher Scientific; AM1908)

(3) Satellite DNA shapes dictate pericentromere packaging in female meiosisNatureFebruary 20, 2025Damian Dudka, Jennine M. Dawicki-McKenna, Xueqi Sun, Keagan Beeravolu, Takashi Akera, Michael A. Lampson, Ben E. BlackNucleoSpin kit (740588.25; Takara). mRNA was prepared using T7 mScript™ Standard mRNA Production System (C-MSC100625; CellScript) in vitro transcription kit. Oocyte microinjection Oocytes were microinjected with ~5

(4) CD32B1, a versatile non-signaling antibody-binding scaffold for enhanced T cell adhesion to tumor stromal cognate antigensFrontiers in immunologyJanuary 1, 2025Feigelson SW, Dadosh T, Levi N, Sapoznikov A, Weinstein-Marom H, Blokon-Kogan D, Avraham Y, Unger T, Gross G, Dahan R, et al.and capping reactions were carried out using T7 mScript Standard mRNA Production System (Cellscript, C-MSC11610). The mRNA product was purified by DNase-I digestion, followed by LiCl precipitation, according to the

(5) Antigen-specific T cell immunotherapy by in vivo mRNA deliverybioRxivNovember 6, 2024Fang-Yi Su, Jamison C. Siebart, Ching S. Chan, Matthew Y. Wang, Xinyi Yao, Aaron Silva Trenkle, Avanti Sivakumar, Melanie Su, Rustin Harandi, Neha Shahrawat, et al.nuclease-free water. IVT was performed using T7 mScript™ Standard mRNA Production System (Cellscript C-MSC11610) following the manufacturer’s protocol with complete substitution of uridine with N1-methyl-pseudouridine.

(6) DNA-PK inhibition enhances gene editing efficiency in HSPCs for CRISPR-based treatment of X-linked hyper IgM syndromeMolecular therapy. Methods & clinical developmentSeptember 12, 2024Pugliano CM, Berger M, Ray RM, Sapkos K, Wu B, Laird A, Ye Y, Thomson D, DeGottardi MQ, Khan IF, et al.

(7) An mRNA-LNP-based Lassa virus vaccine induces protective immunity in miceJournal of virologyJune 13, 2024Hashizume M, Takashima A, Iwasaki M.

(8) T-Cell-Based Platform for Functional Screening of T-Cell Receptors Identified in Single-Cell RNA Sequencing Data Sets of Tumor-Infiltrating T-CellsBio-protocolApril 20, 2024Ehrenfried AR, Zens S, Steffens LK, Kehm H, Paul A, Lauenstein C, Volkmar M, Poschke I, Meng Z, Offringa R.PDA30364-OVA [18] Reagents T7 mScript standard mRNA production system (Cellscript, catalog number: C-MSC100625)RNA 6000 Nano Kit for 2100 Bioanalyzer Systems (Agilent, catalog number: 5067-1511)pcDNATM 3.1(+) (Invitrogen,

(9) Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapyNature BiotechnologyMarch 7, 2024C. L. Tan, K. Lindner, T. Boschert, Z. Meng, A. Rodriguez Ehrenfried, A. De Roia, G. Haltenhof, A. Faenza, F. Imperatore, L. Bunse, et al.resulting PCR product used as a template for the T7 mScript Standard mRNA Production System (CELLSCRIPT C-MSC11610). mRNA was m7G capped and enzymatically polyadenylated following the manufacturer’s instructions. For

(10) FREEDA: An automated computational pipeline guides experimental testing of protein innovationJournal of Cell BiologySeptember 4, 2023Damian Dudka, R. Brian Akins, Michael A. Lampsoncytoplasmic expression. mRNAs were synthesized using the T7 mScriptTM Standard mRNA Production System (C-MSC100625; CELLSCRIPT). Immunofluorescence imaging Oocyte maturation was induced in vitro by washing out milrinone

(11) G-quadruplex in hepatitis B virus pregenomic RNA promotes its translationJournal of Biological ChemistrySeptember 1, 2023Jingjing Wang, Haiyan Huang, Kaitao Zhao, Yan Teng, Li Zhao, Zaichao Xu, Yingcheng Zheng, Lu Zhang, Conghui Li, Yurong Duan, et al.and G4M) were used to produce pgRNA using the T7 mScript Standard mRNA Production System (CELLSCRIPT, C-MSC100625) according to the manufacturer’s instructions. IVT-RNA transfection Huh7-hNTCP cells were seeded in

(12) SARS-CoV-2 nsp13 Restricts Episomal DNA Transcription without Affecting Chromosomal DNAJournal of virologyJuly 27, 2023Li A, Zhang B, Zhao K, Yin Z, Teng Y, Zhang L, Xu Z, Liang K, Cheng X, Xia Y.listed in Table 1. The GFP mRNA was generated by the T7 mScript Standard mRNA Production System (cat no. C-MSC100625, CELLSCRIPT, Madison, WI, USA). The chemicals used in this study are listed in Table 2. Luciferase reporter assay

(13) USH2A Gene Mutations in Rabbits Lead to Progressive Retinal Degeneration and Hearing LossTranslational Vision Science & TechnologyFebruary 16, 2023Van Phuc Nguyen, Jun Song, Diane Prieskorn, Junhuang Zou, Yanxiu Li, David Dolan, Jie Xu, Jifeng Zhang, K. Thiran Jayasundera, Jun Yang, et al.transcribed in vitro, capped and polyadenylated using the T7 mScript Standard mRNA Production System (C-MSC100625; CELLSCRIPT, Madison, WI). Guide RNA (gRNA) was designed using CRISPOR software,36 synthesized as chemically

(14) Discrete regulatory modules instruct hematopoietic lineage commitment and differentiationNature communicationsNovember 23, 2021Georgolopoulos G, Psatha N, Iwata M, Nishida A, Som T, Yiangou M, Stamatoyannopoulos JA, Vierstra J.In vitro transcription of TALEN-mRNA was performed by the T7 mScript™ Standard mRNA Production System (CELLSCRIPT, C-MSC100625), including 5’-capping and poly-A addition reactions. Electroporation was performed as previously described88.

(15) Production of CFTR-ΔF508 RabbitsFrontiers in GeneticsJanuary 22, 2021Dongshan Yang et al.transcribed in vitro, capped and polyadenylated using the T7 mScriptTM Standard mRNA Production System (C-MSC100625, CELLSCRIPT, Madison, WI, United States). Cas9 mRNA and sgRNA were diluted in RNase-free TE buffer (1

(16) Rapid identification and evaluation of neoantigen-reactive T cell receptors from single cells.Journal of ImmunotherapyJanuary 1, 2021Biman C. Paria et al.(IVT). The IVT was performed using T7 mScript Standard mRNA Production System (Cellscript, Cat. No. C-MSC11610). CD4+ or CD8+ Jurkat-NFAT-Luc reporter cell production The Jurkat-NFAT-luciferase cell line was obtained

(17) Rapid identification and evaluation of neoantigen-reactive T cell receptors from single cellsJ ImmunotherJanuary 1, 2021Biman C. Paria, Noam Levin, Frank Lowery, Anna Pasetto, Drew C. Deniger, Maria R. Parkhurst, Rami Yossef, Sanghyun P. Kim, Maria Florentin, Lien Ngo, et al.(IVT). The IVT was performed using T7 mScript Standard mRNA Production System (Cellscript, Cat. No. C-MSC11610). CD4+ or CD8+ Jurkat-NFAT-Luc reporter cell production The Jurkat-NFAT-luciferase cell line was obtained

(18) Extraction of mRNA from Stalled Ribosomes by the Ski ComplexMolecular cellMarch 19, 2020Zinoviev A, Ayupov RK, Abaeva IS, Hellen CUT, Pestova TV.Avian Myeloblastosis Virus Reverse Transcriptase,Promega,M5108 T7 mScript-Standard mRNA Production System,Cellscript,C-MSC100625 BspQI,New England Biolabs,R0712S BstNI,New England Biolabs,R0607S EcoRI,New England Biolabs,R0101S HindIII,New England Biolabs

(19) Production of immunodeficient rabbits by multiplex embryo transfer and multiplex gene targetingScientific ReportsDecember 1, 2017Jun Song, Dongshan Yang, Jinxue Ruan, Jifeng Zhang, Yuqing Eugene Chen, Jie Xuin vitro transcribed, caped and polyadenylated using the T7 mScript™ Standard mRNA Production System (C-MSC100625, CELLSCRIPT, Madison, WI). sgRNAs were in vitro transcribed by using T7-Scribe™ Standard RNA IVT Kit

(20) Identification and characterization of rabbit ROSA26 for gene knock-in and stable reporter gene expressionScientific ReportsJuly 1, 2016Dongshan Yang, Jun Song, Jifeng Zhang, Jie Xu, Tianqing Zhu, Zhong Wang, Liangxue Lai, Y. Eugene Chentranscribed in vitro, capped and polyadenylated using the T7 mScript™ Standard mRNA Production System (C-MSC100625, CELLSCRIPT, Madison, WI). sgRNA was in vitro transcribed by using T7-Scribe™ Standard RNA IVT Kit (C-AS3107,

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T7 mScript™ Standard mRNA Production System V2 from CELLSCRIPT

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T7 mScript™ Standard mRNA Production System V2 from CELLSCRIPT

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