Description
The GDF15: GFRAL [Biotinylated] Inhibitor Screening Chemiluminescent Assay Kit is an ELISA designed to measure the binding between GDF15 (Growth and differentiation factor 15) and GFRAL (Glial-derived neurotrophic factor-family receptor α-like) proteins for screening and profiling applications. This kit comes with enough purified GDF15 (amino acids 197-308) and biotinylated GFRAL (amino acids 19-351(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with GDF15 protein. After blocking, biotinylated GFRAL is added in an optimized assay buffer. Next, unbound biotinylated GFRAL is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of GFRAL binding to GDF15. Need us to run inhibitor screens or profile your compounds? Check out our Immunotherapy Biochemical Screening Services