Fig 1: Phosphorylated MLKL levels change in response to AC100861.1 and TNFRSF10A dysregulation.Graph displaying the fold change of phosphorylated MLKL (phos-MLKL) protein levels in the AC100861.1-KD, AC100861.1-OE, TNFRSF10A-KD, and TNFRSF10A-OE samples compared to control samples. The gray dotted line corresponds to fold change = 1. For all samples, N = 3. Error bars indicate the standard error of the mean.
Fig 2: AC100861.1 and TNFRSF10A transcripts levels do not affect the expression of each other in trans.Graphs indicate the changes in expression (compared to control) of AC100861.1 and TNFRSF10A in ARPE-19 cells in response to siRNA-mediated knockdown (A) and vector overexpression (B), as measured by RT-qPCR. C Graph indicating the protein-level changes (compared to control) of TNFRSF10A in ARPE-19 cells in response to siRNA-mediated knockdown and vector overexpression, as measured by ELISA. The gray dotted line corresponds to fold change = 1. For TNFRSF10A-OE protein-level analysis in (C), N = 2. For all other samples, N = 3. Error bars indicate the standard error of the mean.
Fig 3: Expression of TRAIL receptors shifts after AC100861.1 and TNFRSF10A dysregulation.Graphs indicate the expression level changes (compared to control) in ARPE-19 cells transfected with siRNA targeting AC100861.1 (A), AC100861.1 overexpression vector (B), siRNA targeting TNFRSF10A (C), and TNFRSF10A overexpression vector (D). Expression levels of TNFRSF10B, TNFRSF10C, and TNFRSF10D were measured by RT-qPCR. The gray dotted line corresponds to fold change = 1. For all samples, N = 3. Error bars indicate the standard error of the mean.
Fig 4: Components of inflammatory pathways are affected by AC100861.1 and TNFRSF10A dysregulation.Graphs indicate the expression level changes (compared to control) in ARPE-19 cells transfected with siRNA targeting AC100861.1 (A), AC100861.1 overexpression vector (B), siRNA targeting TNFRSF10A (C), and TNFRSF10A overexpression vector (D). Expression levels of CASP1, IL1A, IL1B, IL18, IL33, NLRP3, and NFKB1 were measured by RT-qPCR. The gray dotted line corresponds to fold change = 1. For all samples, N = 3. Error bars indicate the standard error of the mean.
Fig 5: Schematic of the human TNFRSF10A genetic locus.The AC100861.1 (orange) and TNFRSF10A (teal) genes possess a head-to-head orientation. The inset is a magnification of the promoter regions and the first exons of AC100861.1 and TNFRSF10A. Promoter regions (light blue) are indicated. The rs13278062 SNP (dark blue) falls within the first exon of the AC100861.1 gene.
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