Fig 1: Epithelial receptivity and stromal decidualization in ADOC.(A) Schematic of the experimental setup. (B) Decidualization of stromal cells after 6 days of hormonal treatment. Left: Immunostaining of stromal cells before and after hormonal treatment with F-actin (orange) and DAPI (blue). Right: Morphological quantitative analysis showing increased roundness (top panel) and cellular area (bottom panel) of stromal cells after hormonal treatment. Data represent the quantification of five chips from three independent donors (roundness *P < 0.05 by Mann-Whitney test). Scale bars, 70 μm. (C and D) Presence of IGFBP-1 (purple) and PRL (pink) in response to hormonal treatment, along with DAPI (blue) and F-actin (orange) staining. Scale bars, 50 μm. (E) PRL and IGFBP-1 quantification using ELISA at days 0, 3, and 6 of hormonal treatment. Data are presented as mean ± SD, n = 3 independent chips from 3 different donors, **P < 0.01 by Bonferroni’s multiple comparisons two-way ANOVA. (F) Acetylated α-tubulin (white) and PAEP (red) in EECs before and after hormonal treatment. Scale bars, 10 μm. (G) Quantification of the percentage positive α-acetylated tubulin and PAEP cells before and after hormonal treatment. Data are presented as mean ± SD, of five chips from three independent donors (n = 5), **P < 0.01, Mann-Whitney test for PAEP and *P < 0.05 by unpaired t test for acetylated α-tubulin. All data were tested Shapiro-Wilk normality tests before running parametric or nonparametric tests.