BCA Protein Assay Kit II ab287853 from Abcam

Product Specs
ItemBCA Protein Assay Kit II
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab287853
Quantity2500 Test, 5000 Test
Detection Range25 - 2000 µg/ml

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) WWP2 ubiquitin ligase promotes colorectal cancer progression by targeting p53 for degradation: an experimental studyAnn Surg Treat ResApril 30, 2026Seung-Jun Lee, Han-Gil Kim, Young-Tae Ju, Young-Sool Hah, Jeongyun Hwang, Jihun Choi, Jin-Kyu Cho, Chi-Young Jeong, Young-Joon Lee, Ji-Ho Park, et al.Thermo Fisher Scientific). Protein concentration was measured with a bicinchoninic acid protein assay kit (Cat. No. ab287853, Abcam). Equal protein amounts were separated by SDS-PAGE and transferred to nitrocellulose membranes

(2) Interferon regulatory factor 5 involves the pathogenesis of emphysema through NLRP3 and Ly6C expressing cellsExperimental & Molecular MedicineFebruary 5, 2026Sun-Hee Heo, Suk Young Park, Na Hyun Kim, Heeseo Kim, In-Jeoung Baek, Young Hoon Sung, Chiwook Chung, Sei Won Leeprotein concentrations were measured using bicinchoninic acid protein assay kits (Abcam, catalog number ab287853). Protein samples were separated by 8% SDS–polyacrylamide gel electrophoresis (SDS–PAGE) for NLRP3,

(3) S-palmitoylation of MTDH regulates ferroptosis resistance in breast cancer cellJournal of Lipid ResearchJanuary 1, 2026Shaojun Pei, Wen Wang, Tingze Feng, Qiuping Wang, Yuhan Wang, Hong-Xu Liu, Xinmiao Liang, Hai-long Piaothe protein concentrations of the supernatants were measured using BCA protein assay kit II (Abcam, ab287853). Taking 1 mg total proteins and incubating with 1 mM CuSO4, 1.5 mM TCEP, 100 μM TBTA, and 200 μM biotin-azide

(4) β-catenin initiates peritoneal fibrosis by triggering mitochondrial fission-mediated mesothelial cell senescence fate transitionMil Med ResDecember 1, 2025Xiao-Xu Wang, Wei-Jie Zhong, Jie-Mei Li, Di Wang, Shuang-Qin Chen, Jin-Hua Miao, Wei-Wei Shen, Xiao-Long Li, Jie-Wu Huang, Shan Zhou, et al.visceral peritoneum samples was lysed by RIPA lysis buffer with protease inhibitors. BCA protein assay kit (ab287853; Abcam, USA) was used to quantify the protein lysates. Before running the gels, equal quantities of

(5) Single-cell analysis of the decidua unveils the mechanism of anti-inflammatory exosomes for chorioamnionitis in nonhuman primatesSci AdvJuly 4, 2025Seung Hyun Jang, Hojun Choi, Eun Mi Lee, Seung-Bin Yoon, Hye-Jung Kim, Cheolhyoung Park, Jae Kyung Won, Seung Han Shin, Ji-Su Kim, Chan-Wook Park, et al.concentrations were determined using the bicinchoninic acid method (BCA Protein Assay Kit II, catalog no. ab287853, Abcam). After the protein assay, we performed multiplex cytokines analysis of the fetal lung lysate

(6) Matrix metalloproteinase-10 promotes kidney fibrosis by transactivating β-catenin signalingCell Death DiscovMay 17, 2025Xiaoli Sun, Qian Ren, Xi Liu, Huishi Tan, Zhanji Zhan, Enqing Lin, Yinyi Long, Xue Hong, Lili Zhou, Youhua Liufrom mouse kidney homogenates or cell lysates were quantified using the BCA Protein assay Kit (Cat. ab287853; Abcam, Cambridge, UK). For each lane, 20-40 µg of protein was separated by SDS-PAGE and transferred

(7) Network pharmacology study on the mechanism of berberine in Alzheimer's disease modelNPJ Sci FoodFebruary 4, 2025Yaoyi Zhang, Shuai Lv, Pinyuan Huang, Lingmin Xiao, Nan Lin, En HuangChina) and phosphatase (Cat: K1015, APExBIO, China) inhibitor cocktail. BCA protein assay kit (Cat: ab287853, Abcam, USA) was applied to detect the protein concentration of lysates. After the sample was heated

(8) Anti-Tumor Effect and Mechanism Study of Caloric Restriction, Achieved by Time-Restricted Feeding, in MiceCancer ControlNovember 22, 2024Weisheng Lu, Jue Wang, Chengji Wang, Haijie Wang, Wenhao Gao, Shouchong Ye, Ruling Shenantibody (dilution:1/10000; ab134903), AKT antibody (dilution:1/10000; ab179463), and protein assay kit (ab287853) were purchased from Abcam. Anti-mouse antibodies for EGFR (JM-11695M1), P53 (JM-02303M1), IGF-1 (JM-12005M1),

(9) The Potential Role of SNRPD1 Stabilized by IGF2BP2 in the Progression of Triple-Negative Breast CancerBreast Cancer: Targets and TherapyOctober 11, 2024Siqi Liu, Xin Sun, Na Liu, Fangcai Linthe total protein concentration was measured using a bicinchoninic acid protein assay kit (Abcam, Cat#ab287853). After separation by 8–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins

(10) The Potential Role of SNRPD1 Stabilized by IGF2BP2 in the Progression of Triple-Negative Breast CancerBreast Cancer (Dove Med Press)October 11, 2024Siqi Liu, Xin Sun, Na Liu, Fangcai Linthe total protein concentration was measured using a bicinchoninic acid protein assay kit (Abcam, Cat#ab287853). After separation by 8–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the proteins

(11) High-Moisture Extrusion of a Dietary Protein Blend Impairs In Vitro Digestion and Delays In Vivo Postprandial Plasma Amino Acid Availability in HumansThe Journal of NutritionJuly 1, 2024Sam West, Alistair J Monteyne, Gráinne Whelehan, Doaa R Abdelrahman, Andrew J Murton, Tim JA Finnigan, Giuseppina Mandalari, Catherine Booth, Peter J Wilde, Francis B Stephens, et al.concentration of the digesta during digestion was quantified by bicinchoninic acid (BCA) assay (Abcam, ab287853) as previously described [45]. Briefly, 10 μL of standard or sample were incubated with 200 μL working

(12) Puerarin attenuates remifentanil‑induced postoperative hyperalgesia via targeting PAX6 to regulate the transcription of TRPV1Mol Med RepMarch 19, 2024Libang Yuan, Yinghai Liu, Yangyang Sun, Ling Ren, Xiaoping Gu, Liang Chen, Gongrui Zhou, Xiaoqin Sun, Qingqing Huang, Xufei Chen, et al.extract the protein from spinal cord tissue and protein concentration was determined by BCA kit (cat. no. ab287853; Abcam). As previously reported (13), western blot assay was performed in accordance with standardized

(13) m(6)A RNA methylation drives kidney fibrosis by upregulating β-catenin signalingInternational journal of biological sciencesJanuary 1, 2024Long Y, Song D, Xiao L, Xiang Y, Li D, Sun X, Hong X, Hou FF, Fu H, Liu Y.or cultured cells were prepared. The protein concentration was determined using the BCA assay (Cat. ab287853; Abcam). Protein samples were reduced, denatured, and subjected to Western blot analysis with specific

(14) Super-resolution imaging unveils the self-replication of tau aggregates upon seedingCell reportsJuly 25, 2023Dimou E, Katsinelos T, Meisl G, Tuck BJ, Keeling S, Smith AE, Hidari E, Lam JYL, Burke M, Lövestam S, et al.Viability Reagent,Thermo Fisher,Cat# A13261 Human Tau ELISA kit,Abcam,Cat# ab273617 BCA assay,Abcam,Cat# ab287853 CytoTox 96 Non-Radioactive Cytotoxicity Assay,Promega,Cat#G1780 SiteClick™ Antibody Azido Modification

(15) USP51 promotes non-small cell lung carcinoma cell stemness by deubiquitinating TWIST1Journal of translational medicineJuly 8, 2023Chen J, Wu Z, Deng W, Tang M, Wu L, Lin N, Chen L, Fu Y, Zhao M, Chen C, et al.(Beyotime, P0013B) and the protein concentration was determined using a BCA protein assay kit II (Abcam, ab287853). Proteins were transferred onto a PVDF membrane after separation by 10% SDS‒PAGE, and 5% skim milk

(16) Macrophage promotes fibroblast activation and kidney fibrosis by assembling a vitronectin-enriched microenvironmentTheranosticsJanuary 1, 2023Peng Y, Li L, Shang J, Zhu H, Liao J, Hong X, Hou FF, Fu H, Liu Y.were prepared with RIPA buffer. BCA assay was used to measure the concentration of total protein (Cat. ab287853, Abcam). Protein samples were reduced, denatured, subjected to Western blot analysis. The protein bands

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BCA Protein Assay Kit II from Abcam

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BCA Protein Assay Kit II from Abcam

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