MACSPlex EV Kit IO, human 130-122-209 from Miltenyi Biotec

(1) Plasma Extracellular Vesicles-Derived Complement Proteins as Biomarkers of Sarcopenia Progression in Longitudinal CohortsJ Cachexia Sarcopenia MuscleJuly 20, 2026Ji Yeon Kim, Sung Hye Kong, Dohyun Han, Tae-Hwan Gil, Hyeon Soo Kim, Miji Kim, Jin Hee Kim, Seung Shin Park, Seong-Jun Park, Hak Chul Jhang, et al.Marker Analysis Surface marker profiling of EVs was performed using the MACSPlex EV kit IO (human; #130‐122‐209, Miltenyi Biotec, Germany), following the manufacturer's instructions. The overnight incubation protocol

(2) Donor Pooling as an Effective Method to Increase MSC EV Production Without Compromising Therapeutic PotentialJ Extracell BiolJuly 13, 2026Rebecca Davies, Claire Mennan, Anaïs Makos, Tian Lan, Charlotte Hulme, Mark Platt, Karina Wright, Oksana Kehoeequivalent of 6 µg protein per sample was inputted into the MACSPlex Exosome kit (Miltenyi Biotec, 130‐122‐209), as per the manufacturer's recommendations. Briefly, EV enrichments (57 µL of donor 2, 45 µL of pooled

(3) Extracellular Vesicles from Glioblastoma Cells Reflect 2D vs. 3D Culture Adaptation and Resistance to TemozolomideMolecular & Cellular ProteomicsJune 24, 2026Getulio Pereira de Pereira de Oliveira, Alan Zimmerman, Cintia C. Palu, Vivian Tran, Gabrielle Bogut, Stephanie Chidester, John Tigges, Rafael A. Vega, Elena Aikawa, Jennifer Jones, et al.Assay EV samples from 2D and 3D cultures were analyzed with the MACSPlex EV Kit IO (for immuno-oncology) (#130-108-813) (Miltenyi Biotec). EVs were incubated with an antibody-capture bead mixture containing 39 unique fluorescently

(4) High-Dimensional Immunophenotyping of Plasma-Derived Small Extracellular Vesicles in Pancreatic Cancer: An Exploratory Proof-of-Principle StudyBiomoleculesJune 24, 2026Sabrina Sulzer, Johanna Lisa Becker, Laura Domogalla, Volker Ellenrieder, Matthias Schulz, Markus Maulhardt, Alexander Casimir Angleitner, Judith BüntzelFlow cytometry. Surface marker profiling of sEVs was performed using the MACSPlex EV Kit IO, human (order no. 130-122-209/130-108-813, Miltenyi Biotec, Bergisch Gladbach, Germany), which allows simultaneous flow-cytometric

(5) Mesenchymal stem cell derived exosomes mitigate COVID-19 cytokine storm via Annexin A1 and TGF-β mediated MAPK pathway inhibitionStem Cell Research & TherapyMay 8, 2026Nesrine Ebrahim, Hajir A. Al Saihati, Arigue A. Dessouky, Yasmeen Mohammed Ismail, Ashraf A. Shamaa, Shereen A. Mohamed, Mohamed E. Mohamed, Nermine Nosseir, Mohamed Ahmed Eladl, Gianpiero Di Leva, et al.used a bead-based multiplex flow cytometry approach (MACSPlex Exosome Kit, Miltenyi Biotec, Cat. No. 130-108-813). The exosomes were cleaned and resuspended in phosphate-buffered saline (PBS) supplemented with 3%

(6) Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell SurvivalCellsApril 18, 2026Cuiping Zhang, Peng Huang, Matthew Pak, Jennifer A. Korchak, Abba C. Zubairanalyzed using the MACSPlex Exosome Kit (human) (Miltenyi Biotec, Bergisch Gladbach, Germany, catalog#130-108-813) following the manufacturer’s protocols. EV samples derived from VEGF-MSCs and control MSCs were thawed

(7) Cholesteryl esters and high protein-to-lipid ratios distinguish non-vesicular extracellular particles from extracellular vesiclesCell Communication and SignalingApril 17, 2026Sayam Ghosal, Rita Leporati, Árpád Varga, Petra Susanszki, Nóra Fekete, Tamás László, Tünde Bárkai, Fülöp Károly Grébecz, Delaram Khamari, Tárek Zoltán Magyar, et al.assay Bead-based multiplex analysis was conducted using the MACSPlex EV Kit IO human (catalogue # 130-122-209, Miltenyi Biotec, Germany). Assays were performed as described earlier [31, 32]. EV and NVEP samples

(8) Ultracentrifugation and Ultrafiltration Differentially Alter the Composition and Functionality of the Biomolecular Corona of Extracellular VesiclesJ Extracell BiolApril 1, 2026Frederic St-Denis-Bissonnette, Hala Halabi, Marybeth Creskey, Melanie Kirkby, Xu Zhang, Dylan Burger, Lisheng Wang, Jessie R. LavoieNK‐EVs Immunophenotyping of EV surface antigens was performed using the human MACSPlex EV Kit IO (Miltenyi Biotec, cat#130‐108‐813), a multiplex bead‐based flow cytometry assay, following the same procedure as described in our previous

(9) Spectroscopic fingerprinting of extracellular vesicles from diverse cellular origins by ATR-FTIR for vibrational biomarkers of vector-host interactionsSci RepMarch 16, 2026Emine Billur Sevinis Ozbulut, Kenta Hoshino, Yoshitomo Furushima, Yuki Yoshida, Takashi Yamamoto, Hanna Reßin, Shreyans Chatterjee, Jan Munch, Boris Mizaikoff, Rüdiger M. Groß, et al.preparation. EV surface marker profiling was performed using the Miltenyi Biotec MACSplex EV IO Kit (#130-108-813) combined with Alexa Fluor 647–conjugated bovine lactadherin (CellSystems) for phosphatidylserine (PS)

(10) Immune Cell-Specific and Isoform-Selective Regulation of CD44 in Pancreatic Ductal Adenocarcinoma Links Lymph Node Variant Loss and Exosomal CD44 to Clinical Outcome in Pancreatic Ductal AdenocarcinomaCellsFebruary 27, 2026Alara Karabiber, Yong Zhou, Anke Mittelstädt, Frederik Johannes Hansen, Melanie Litau, Isabelle Kuchenreuther, Johanne Mazurie, Finn Niklas Clausen, Sebastian Klöckner, Franziska Czubayko, et al.MACSPlex Exosome Assay Exosome analysis was performed using the MACSPlex Human Exosome Kit (Cat. No. 130-108-813, Miltenyi Biotec, Bergisch Gladbach, Germany), as previously described [20,21]. The assay utilizes 4–8

(11) Pluripotent stem cell–derived extracellular vesicles for systemic immune modulation in diabetes therapyResearch SquareFebruary 6, 2026Song Li, Jana Zarubova, Mohammad Hasani-Sadrabadi, Yutong Wu, Graciel Diamante, Jenny Cheng, Xiao Han, Fatemeh Majedi, Li Yang, Olivia Wang, et al.profiles. Evaluation of EV surface markers To assess EV surface markers, the MACSPlex Exosome Kit (130-122-209, Miltenyi Biotec) was employed, following the manufacturer’s guidelines. EV samples were incubated with

(12) Enrichment of Immune Cell‐Derived Extracellular Vesicles From Plasma Using 35 and 70 nm Size‐Exclusion Chromatography Columns of Different SizesJournal of Extracellular BiologyNovember 11, 2025Iris Potakowskyj, Ivan Bagarić, Nevena Prodanović, Adrián Parrilla Mesas, Olga Glatki, Klaus Heukamp, Kenneth W. Witwer, Hanns‐Martin Lorenz, Lars‐Oliver Tykocinski, Aoife Ward Gahlawat, et al.the Supporting Information. 2.8 Multiplex EV Surface Marker Analysis The MACSPlex EV Kit IO, human (130‐108‐813, Miltenyi) was used for surface marker detection of EVs isolated from both large and small columns according

(13) Multicomponent-based analyses of ACS-patient-derived extracellular vesicles as likely tools for tailored interventional approachesiScienceOctober 17, 2025Femminò S, Sarcinella A, Grosso A, Mensitieri F, De Filippo O, Bruno F, Coglianese A, Tapparo M, Pace E, Kholia S, et al.assays,Critical commercial assays,Critical commercial assays ,, MACSPlex Exosome Kit,Miltenyi Biotec,130-108-813 miRNeasy® Mini Kit,Qiagen,217084 miScript II RT Kit,Qiagen,205213 miScript SYBR® Green PCR kit,Qiagen,330502 In-Solution Tryptic Digestion and Guanidination Kit

(14) Nasal Epithelial Extracellular Vesicles Correlate with Type 2 Inflammation during Aspirin-induced Respiratory ReactionsAmerican Journal of Respiratory Cell and Molecular BiologyOctober 1, 2025Kaitlyn E. Bunn, Zachary J. Bressman, Grant K. Nation, Pingsheng Wu, Taneem Amin, Courtney Lehman, Anabel Lin, Carleigh J. Gray, Tanya M. Laidlaw, Katherine N. Cahill, et al.Thermo Fisher Scientific) at 22°C with mixing at 450 rpm for 2 hours. MACSPLEX Exosome Capture Beads (130-108-813; Miltenyi Biotec) conjugated to antibodies against surface markers expressed by immune cells (CD1c,

(15) GMP-Compliant Process for the Manufacturing of an Extracellular Vesicles-Enriched Secretome Product Derived From Cardiovascular Progenitor Cells Suitable for a Phase I Clinical TrialJ Extracell VesiclesAugust 20, 2025Camille Humbert, Chloé Cordier, Iouri Drut, Michele Hamrick, Jacquelyn Wong, Valérie Bellamy, Justine Flaire, Kiranmayee Bakshy, Florent Dingli, Damarys Loew, et al.of particles in the EV‐enriched secretome preparations, the MACSPlex Exosome Kit (Miltenyi Biotec, #130‐108‐813) was used following the manufacturer's instructions. In this kit, 37 surface epitopes and two isotype

(16) Anti-Her2 CAR-NK92 Cells and Their Exosomes: Generation, Characterization, and Selective Cytotoxicity Against Her2-Positive Tumor CellsInt J Mol SciAugust 7, 2025Alexandru Tîrziu, Florina Maria Bojin, Oana Isabella Gavriliuc, Roxana Maria Buzan, Lauriana Eunice Zbîrcea, Manuela Grijincu, Virgil Păunescutime. Exosomal surface proteome was assessed using the MACSPlex EV IO Detection Kit (Miltenyi Biotec, cat. #130-108-813, Bergisch Gladbach, Germany) by incubating the exosomal suspension with 15 µL of exosome capture beads

(17) Human milk extracellular vesicles modulate inflammation and cell survival in intestinal and immune cellsPediatric ResearchJuly 1, 2025Brett Vahkal, Illimar Altosaar, Ardeshir Ariana, Josie Jabbour, Falia Pantieras, Redaet Daniel, Éric Tremblay, Subash Sad, Jean-François Beaulieu, Marceline Côté, et al.prepared for the overnight protocol following manufacturer’s instructions (MACSPlex Exosome Kit, Cat. No. 130-108-813, Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly, 10 μg/mL of EVs, 120 μL of capture beads, or

(18) Identification of distinct profiles of glioblastoma through the immunocapture of extracellular vesicles from patient plasmaPLoS OneMarch 19, 2025Doina Ramona Manu, Rodica Bǎlaşa, Lavinia-Lorena Pruteanu, Victor Curean, Lucian Barbu-Tudoran, Georgiana-Mihaela Şerban, Rareş Chinezu, Adrian Bǎlaşamultiplex analysis in FACS Aria III flow cytometer with MACSPlex Exosome Kit (Miltenyi Biotec, cat. no. 130-122-209). As it was previously evaluated, this method can detect EV surface marker signatures in a specific

(19) Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis PathogenesissJ Extracell VesiclesMarch 17, 2025Shanti Gurung, Jacqueline Piskopos, Joel Steele, Ralf Schittenhelm, Anup Shah, Fiona L. Cousins, Thomas T. Tapmeier, Caroline E. GargettMarkers by Flow Cytometry MF‐sEV surface epitope expression was analysed using MACSPlex Exosome Kit (#130122209, Miltenyi Biotec). Twenty microgram of endometriosis or control MF‐sEVs were diluted in MACSPlex buffer

(20) Nasal Epithelial Extracellular Vesicles Correlate with Type 2 Inflammation during Aspirin-induced Respiratory ReactionsAm J Respir Cell Mol BiolMarch 14, 2025Kaitlyn E. Bunn, Zachary J. Bressman, Grant K. Nation, Pingsheng Wu, Taneem Amin, Courtney Lehman, Anabel Lin, Carleigh J. Gray, Tanya M. Laidlaw, Katherine N. Cahill, et al.Thermo Fisher Scientific) at 22°C with mixing at 450 rpm for 2 hours. MACSPLEX Exosome Capture Beads (130-108-813; Miltenyi Biotec) conjugated to antibodies against surface markers expressed by immune cells (CD1c,

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MACSPlex EV Kit IO, human from Miltenyi Biotec

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MACSPlex EV Kit IO, human from Miltenyi Biotec

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