Description
The Cathepsin B Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin B (amino acids 18-339), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control. The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease