Description
A non-radioactive cytotoxicity assay allowing phenotyping of a heterogeneous target cell population and detection of cytolytic activity at the cellular level, using flow cytometry.
The immune system is capable of recognizing and destroying target cells, such as tumor or intracellular pathogen infected cells, through a process known as cell mediated cytotoxicity (CMC) or antibody-dependent cell-mediated cytotoxicity (ADCC). Evaluation of this CMC/ADCC activity is one of the most important immunoassays to monitor the status of the immune system. The most commonly used method to measure CMC/ADCC is a radioactive chromium-51 (51Cr) release assays. There are several disadvantages with this assay in that it is expensive, difficult to load certain cell types, strict environmental regulations makes waste disposable expensive and spontaneous release of 51Cr results in high