Description
A rapid, no-wash assay to measure H2O2, based on the formation of a fluorescent product. The kit can measure H2O2 release from cells or enzyme coupled reactions. It can also measure peroxidase activity.
The kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions