Fig 1: Effect of PPI-ImmTAAI on NK92-PD-1 inflammatory effector functions. (A–G) NK92-PD-1 cells were stimulated for 20 h with K562-HLA-A*02-PPI in the presence of ctr-ImmTAAI (grey) or PPI-ImmTAAI (red). (A) Graph summarising GrzB concentration in the culture supernatant in the presence of 10 nM ImmTAAI molecules. Each dot represents one sample (mean; n = 6; four independent experiments; paired t-test; **p ≤ 0.01). (B) Graphs summarising IFN-γ, TNF-α, and GM-CSF concentrations in the culture supernatant in the presence of 10 nM ImmTAAI molecules. Each dot represents one sample (mean; n = 6; three independent experiments; paired t-test; ***p ≤ 0.001; ****p ≤ 0.0001). (C) Graphs summarising IFN-γ, TNF-α, and GM-CSF concentrations in the culture supernatant in the presence of different ImmTAAI molecules, normalised to 0 nM ImmTAAI values. Each dot represents the mean of three independent experiments (n = 6; two-way ANOVA; SD; *p ≤ 0.05; ****p ≤ 0.0001). (D) Graph summarising CXCL1 concentration in the supernatants of activated NK92-PD-1 cells (triangles), NHDF cells (squares), or NHDF cells activated with NK92-PD-1 supernatant (circles). Each dot represents one sample (mean; n = 6–24; two independent experiments; one-way ANOVA; ****p ≤ 0.0001). (E) Graph summarising CXCL1 concentrations in the supernatant of NHDF cells stimulated with supernatants from activated NK92-PD-1/ctr-ImmTAAI cells (grey) or activated NK92-PD-1/PPI-ImmTAAI cells (red). Each dot represents one sample (mean; n = 6; three independent experiments; paired t-test; ****p ≤ 0.0001). (F) Graph representing the ratio of EndoC-β-cell number over time, normalised to 0 h and cultured in the presence of resting NK92-PD-1 supernatant (black), activated NK92-PD-1/ctr-ImmTAAI supernatant (grey), or activated NK92-PD-1/PPI-ImmTAAI supernatant (red) (representative of three independent experiments; SD). (G) Graph summarising the ratio of EndoC-β-cell number at 88 h, normalised to 0 h, and cultured in the presence of resting NK92-PD-1 supernatant (black), activated NK92-PD-1/ctr-ImmTAAI supernatant (grey), or activated NK92-PD-1/PPI-ImmTAAI supernatant (red). Each dot represents one sample (mean; n = 7; three independent experiments; one-way ANOVA; ***p ≤ 0.001; ****p ≤ 0.0001).