Description
The E.coli HCP ELISA Detection Kit is applicable for assay of E. coli HCP in test samples by double-antibody sandwich method. The standard and test samples are added to reaction wells on microplate pre-coated with antibody for incubation. The present HCP quantitatively binds to the antibodies in the microplate, while unbound substances are removed after plate washing. Add Anti-HCP-Biotin and Streptavidin HRP successively to form an antibody - antigen - biotinylated antibody - enzyme-labeled avidin complex. The protein content in the test sample can be indicated by the color development degree of TMB. Please read the instructions for use carefully and check the components of the kit before use