Mitochondrial Membrane Potential Assay Kit (with JC-1) E-CK-A301 from Elabscience Bionovation Inc.

Product Specs
ItemMitochondrial Membrane Potential Assay Kit (with JC-1)
CompanyElabscience Bionovation Inc.
Price $108.00Supplier Page View Company Product Page
Catalog NumberE-CK-A301
DetectionFluorometric method;Flow cytometry
Sample TypeCell samples
StorageThis product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components
Assay Time40min
Quantity20 Assays

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Elabscience Bionovation Inc.

14780 Memorial Drive

Suite 105

Houston, Texas 77079

United States

Phone: 1-832-243-6086
Toll-free: 1-888-852-8623
Fax: 1-832-243-6017

Email: techsupport@elabscience.com

Company Profile

Website: www.elabscience.com

(1) Sirtuin 5-mediated desuccinylation of PRDX6 inhibits ferroptosis and alleviates sepsis-associated acute kidney injuryRedox RepDecember 31, 2026Wenlong Lin, Caitao Dong, Qinhong Jiang, Yuanquan Lou, Long Wang, Ziqi Heused (Elabscience, Wuhan, China). Mitochondrial membrane potential assay (JC-1) The JC-1 reagent (E-CK-A301, Elabscience, China) was used to measure mitochondrial membrane potential. Cells were incubated with

(2) miR-423–5p/NDUFS7-mediated mitochondrial function modulation contributes to quercetin-induced attenuation of pulmonary fibrosis via extracellular matrix remodeling regulationNon-coding RNA ResearchJune 1, 2026Lang Xia, Jing Cai, Xueqin Zeng, Qian Shu, Qiong Hu, Shanshan Rao, Jing Han, Weijia Liureached the appropriate confluency. A Mitochondrial Membrane Potential Detection Kit (JC-1) (Cat. No. E-CK-A301, Elabscience) was used according to the provided protocol. JC-1 working solution was added to completely

(3) Resveratrol Attenuates Liver Inflammation in Non-Alcoholic Fatty Liver Disease by Activating PINK1-Mediated MitophagyAnimals (Basel)March 27, 2026Shujing Tan, Ran Yu, Longwei Sun, Manman Shen, Juan Framirez Pedroso, Osmani Chacón Chacón, Chengmin Li, Weiguo Zhao(Δψm) The assessment of mitochondrial membrane potential (Δψm) was conducted using a JC-1 Assay Kit (E-CK-A301, Elabscience, Wuhan, China). Following treatments, AML-12 cells (2 × 105/well) were subjected to washing

(4) Cyclic guanosine monophosphate-protein kinase G signaling attenuates aortic valve calcification through ULK1-mediated autophagySignal Transduct Target TherMarch 12, 2026Yan Wang, Fei Xu, Chengxiang Song, Xi Wang, Hao Zhou, Tianyi Qu, Hongde Li, Qiang Luo, Wenhua Lei, Yue Yin, et al.with osteogenic differentiation medium with or without vericiguat via a JC-1 assay kit (Elabscience, E-CK-A301, China) according to the manufacturer’s protocol. Briefly, VICs were collected, washed twice with PBS,

(5) COX7A1-mediated mitochondrial dysfunction can induce ferroptosis in endometrial cancer cellsPLOS OneFebruary 23, 2026Qi Wu, Suning Bai, Liyun Song, Lina Han, Pei Wangspecified for each experimental group. Following the manufacturer’s instructions for the JC-1 staining kit (E-CK-A301, Elabscience, China), the JC-1 working solution was added, and the cells were incubated for 30 minutes

(6) Ferroptosis inhibits cementoblast mineralization via cGAS-STING/GPX4 axisProgress in OrthodonticsJanuary 13, 2026Tian Wei, Dongyang Li, Jie Zhang, Zhe Zhou, Chunmiao Jiangselected areas. Mitochondrial membrane potential was monitored using the JC-1 detection kit (Elabscience, E-CK-A301). JC-1 is a lipophilic dye that enters living cells and concentrates in the mitochondria. Healthy mitochondria

(7) Antioxidant taurine inhibits chondrocyte ferroptosis through upregulation of OGT/Gpx4 signaling in osteoarthritis induced by anterior cruciate ligament transectionJournal of Advanced ResearchNovember 1, 2025Xuchang Zhou, Yajing Yang, Xu Qiu, Huili Deng, Hong Cao, Tao Liao, Xier Chen, Caihua Huang, Donghai Lin, Guoxin Niphotography. Mitochondrial membrane potential detection Operations were performed according to the kit (Elabscience, E-CK-A301) instructions [48]. Primary chondrocytes were inoculated into 12-well plates. 1 ml of cell culture medium

(8) Garcinone D mitigates amyloid β42-Induced neurotoxicity: unravelling mechanisms of neuroprotectionScientific ReportsSeptember 30, 2025Hin Yee Thew, Wei Meng Lim, Yong Sze Ong, Lin Kooi Ong, Marie-Odile Parat, Bey Hing Goh, Kooi Yeong Khaw(MMP) measurement The integrity of the mitochondria was observed using the JC-1 dye (Elabscience, #E-CK-A301). JC-1 dye is a cationic dye that has the property of accumulating in mitochondria in a potential-dependent

(9) Chrysin sensitizes glioblastoma cells and spheroids to temozolomide treatment by reducing EMT and stemness phenotypes, as well as targeting multidrug resistance proteinsFrontiers in PharmacologySeptember 2, 2025Yunus Aksüt, Aslıhan Şengelen, Dudu Melek Gürsoy, İrem Öğütcü, Özge Kuvet, Murat Pekmezslides and treating with CHR and TMZ, cells were incubated with JC-1 dye loading solution (1 μg/mL, #E-CK-A301, Elabscience/Beijing-China) for 20-min/37 °C, and then washed with D-PBS. Slides were examined using

(10) Human amniotic epithelial stem cell exosomes regulate chondrocyte ferroptosis through ACTA2-AS1 targeted binding to ACSL4 for osteoarthritis interventionResearch (Wash D C)August 8, 2025Xiaofei Wang, Zhimin Wu, Lei Xu, Linbing Lou, Yuxia Yang, Jian Zhang, Haixiang Miao, Cunyi Xia, Zhiwei Peng, Dongsheng Yang, et al.chondrocytes was evaluated using fluorescence microscopy with JC-1 Mitochondrial Membrane Potential Assay Kit (E-CK-A301, Elabscience). Treated cells were incubated with JC-1 staining solution for 20 min at 37 °C and analyzed

(11) CIRBP Stabilizes Slc7a11 mRNA to Sustain the SLC7A11/GPX4 Antioxidant Axis and Limit Ferroptosis in Doxorubicin-Induced CardiotoxicityAntioxidants (Basel)July 29, 2025Yixin Xie, Yongnan Li, Yafei Xie, Jianshu Chen, Hong Ding, Xiaowei ZhangWaltham, MA, USA). 2.8. Laser Confocal Detection of Mitochondrial Membrane Potential JC-1 staining (E-CK-A301, Elabscience, Wuhan, China) in confocal dishes was performed following the manufacturer’s protocol;

(12) NDP52 deficiency accelerates chondrocyte degeneration through promoting pathogenic mitochondrial ROS via reverse electron transportRedox BiolJuly 3, 2025Yutao Zhu, Yaohan Xu, Dinqi Xie, Nengfeng Yu, Jiaxin Chen, Jiechao Xia, Zixuan Mei, Yang Jin, Chuan Hu, Pan Tang, et al.manufacturer's instructions. 2.14 JC-1 detection Mitochondrial Membrane Potential Assay Kit (with JC-1) (E-CK-A301, Elabscience) was used to detect mitochondrial membrane potential. Cells were stained according to manufacturer's

(13) Combination of Berberine and Evodiamine Alleviates Obesity by Promoting Browning in 3T3-L1 Cells and High-Fat Diet-Induced MiceInternational Journal of Molecular SciencesApril 28, 2025Huiying Zhang, Peiyu Xiong, Tianyan Zheng, Youfan Hu, Pengmei Guo, Tao Shen, Xin Zhoueach kit. 4.7. JC-1 Flow Cytometry Analysis The JC-1 Mitochondrial Membrane Potential Assay Kit (E-CK-A301, Elabscience, Wuhan, China) was employed to evaluate mitochondrial membrane potential following the

(14) Caerin 1.1 and 1.9 peptides induce acute caspase 3/GSDME-mediated pyroptosis in epithelial cancer cellsSci RepApril 18, 2025Yuandong Luo, Junjie Li, Quanlan Fu, Pingping Zhang, Xinyi Song, Mengqi Liu, Rongmi Mo, Jiawei Fu, Shuxian Tang, Jialing Wu, et al.purchased from Beyotime Biotechnology. The Mitochondrial Membrane Potential Assay Kit (with JC-1) (E-CK-A301) was obtained from Elabscience. Human IL-1β/IL-1F2 Valukine™ ELISA (VAL101) and Human Total IL-18 Valukine™

(15) Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell ApoptosisBiomoleculesApril 7, 2025Tingji Yang, Bo Fang, Yan Chen, Dan Bao, Jiang Zhang, Peiwen Liu, Zhiwei Duan, Yuxuan He, Xingxu Zhao, Quan-Wei Zhang, et al.of Mitochondrial Membrane Potential JC-1 working fluid was prepared according to the instructions (E-CK-A301, Elabscience, China). When the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial

(16) Functional Attributes of Synovial Fluid from Osteoarthritic Knee Exacerbate Cellular Inflammation and Metabolic Stress, and Fosters Monocyte to Macrophage DifferentiationBiomedicinesApril 4, 2025Vanshika Srivastava, Abhay Harsulkar, Shama Aphale, Aare Märtson, Sulev Kõks, Priya Kulkarni, Shantanu Deshpandemitochondrial membrane potential assay kit (with JC-1) (Elabscience, Houston, TX, USA; catalog No.—E-CK-A301) was used as per the manufacturer’s instructions. JC-1 is a fluorescent probe that gives a red fluorescence

(17) Fatty acid synthase inhibitor cerulenin attenuates glioblastoma progression by reducing EMT and stemness phenotypes, inducing oxidative and ER stress response, and targeting PI3K/AKT/NF-κB axisMed OncolMarch 25, 2025Murat Pekmez, Şefika Beyza Mete, Yunus Aksüt, İrem Öğütcü, Fatma Nur Baştürk, Yusuf Can Gerçek, Aslıhan Şengelenchambered slides were incubated with JC-1 dye (1 μg/mL, 20 min/37 °C) according to the MtMP-kit protocol (#E-CK-A301, Elabscience, Beijing/China). Slides were then washed with D-PBS and examined by fluorescence microscopy

(18) Pyruvate kinase M2 sustains cardiac mitochondrial quality surveillance in septic cardiomyopathy by regulating prohibitin 2 abundance via S91 phosphorylationCellular and Molecular Life SciencesDecember 1, 2024Yingzhen Du, Jialei Li, Zhe Dai, Yuxin Chen, Yao Zhao, Xiaoman Liu, Tian Xia, Pingjun Zhu, Yijin Wangmitochondrial membrane potential quantification, HL-1 cells were incubated in media containing 10 nM JC-1 (#E-CK-A301; Elabscience, Houston, TX, USA) at 37 °C for 10 min. Images were captured under a fluorescence microscope

(19) Recent progress of methods for cuproptosis detectionFrontiers in Molecular BiosciencesSeptember 4, 2024Ligang Zhang, Ruiting Deng, Raoqing Guo, Yawen Jiang, Yichen Guan, Caiyue Chen, Wudi Zhao, Guobin Huang, Lian Liu, Hongli Du, et al.al. (2021) KGDH,Abcam #ab185440,Colorimetric,Atlante et al. (2018) Membrane potential,Elabscience #E-CK-A301,Fluorescent,Xiao et al. (2023) Mitochondrial complex I,Elabscience #E-BC-K149-M,Colorimetric,— Mitochondrial

(20) An investigation of the molecular characterization of the tripartite motif (TRIM) family and primary validation of TRIM31 in gastric cancerHuman GenomicsJuly 9, 2024Yixin Ding, Yangyang Lu, Jing Guo, Shuming Chen, Xiaoxi Han, Shibo Wang, Mengqi Zhang, Rui Wang, Jialin Song, Kongjia Wang, et al.Sigma-Aldrich: D6883), Mitochondrial Membrane Potential Assay Kit (Elabscience Biotechnology Co.,Ltd: E-CK-A301), BeyoClick ™ EdU-488 Cell Proliferation Detection Kit (Beyotime: C0071S), HRP Goat Anti Rabbit IgG (H + L

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Mitochondrial Membrane Potential Assay Kit (with JC-1)

Mitochondrial Membrane Potential Assay Kit (with JC-1)

Mitochondrial Membrane Potential Assay Kit (with JC-1) from Elabscience Bionovation Inc.

Elabscience Bionovation Inc.

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Mitochondrial Membrane Potential Assay Kit (with JC-1) from Elabscience Bionovation Inc.

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