Description
Micronbrane Medical’s proven Pathogen Real-Time Identification by Sequencing, PaRTI-Seq™ assay combines the Devin™ host depletion filter and enrichment kits, the Unison Ultralow DNA or RNA library kits, with the PaRTI-Cular bioinformatics software to enhance microbial reads for any liquid biopsy. By starting with superior host depletion, sequencing costs and complexity are reduced making the PaRTI-Seq assay a precise and economical way to get comprehensive microbial information within 24 hours. We are innovating metagenomics from collection to action. See the data.
Devin Host Depletion Filter. The patented anti-adhesion technology, called Zwitterionic Interface Ultra Self-assemble Coating (ZISC), depletes host nucleated cells while allowing bacterial, viral, fungi, and parasite communities to pass through unaltered.
- < 5 min processing time
- >99% leukocyte depletion
- >90% microbial output
- NOT based on size exclusion
Devin Microbial Enrichment Kit The Devin Microbial DNA Enrichment Kit is a fast and simple genomic nucleic acid purification protocol specifically designed using mNGS-grade reagents to minimize contamination.
- 10 - 1000 fold microbial enrichment
- Process samples within 1.5 hours
- Optimizes extraction conditions for both Gram- and Gram+ bacteria
- Produces high-quality, high-yield genomic DNA
Unison Ultralow DNA/RNA Library Kit optimize the preparation of NGS libraries for various applications such as whole genome sequencing, targeted sequencing by capture, and metagenomic sequencing. In early 2024, we will introduce an RNA library kit and kits optimized for a variety of sequencers. Our current kit can generate libraries from as little as 10 pg of DNA and accelerates DNA library preparations by utilizing a Tn5-based method which condenses fragmentation, adapter ligation, and normalization into a single step for a total processing time of 150 minutes.
PaRTI-Cular bioinformatics software. PaRTI-Cular’s state-of-the-art computational algorithms provide a complete report with automatic data quality checks, removal of host genome reads, pathogen identification, and statistical analyses.
- <30 mins / sample analysis and reporting
- Automatic cutoff of background noise
- Thoroughly curated and regularly updated database comprising over 1400 pathogens
- Proprietary reference database (healthy individuals baseline) for comprehensive analysis
- Genome coverage for pathogens with highest concentration
- Data analysis featuring abundance distributions for infected individuals vs normal (healthy) individuals