RNAqueous Total RNA Isolation Kit AM1912 from Thermo Fisher Scientific

Product Specs
ItemRNAqueous Total RNA Isolation Kit
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberAM1912
Quantity50 Preps
ApplicationsRT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Sample TypeCells and tissues

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Mitochondrial protection role of oxidoreductase-like domain containing 1 in myocardial cells under hypoxiaMedical Gas ResearchJune 1, 2026Yan Yan, Min Dong, Liuyang Tian, Chao Zhu, Xiaojing Zhaocells using the RNAqueous® Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA; AM1912).8 Reverse transcription-quantitative polymerase chain reaction (PCR) was conducted using the PrimeScriptTM

(2) Elevated Metastasis-Associated lung adenocarcinoma transcript 1 (MALAT1) expression predicts coronary artery disease severity as a potential biomarker for risk stratification: a cross-sectional studyAnn MedApril 30, 2026Jia Zheng, Arimi Fitri Mat Ludin, Nor Fadilah Rajab, Nurul Farhana JufriTotal RNA was extracted from plasma samples using RNAqueousTM total RNA isolation kit (Invitrogen, AM1912) optimized for cell-free RNA recovery. Briefly, plasma samples were lysed in a chaotropic buffer to

(3) Taurocholic acid induces intrahepatic cholangiocyte cell proliferation via activating NRAS and YAP1PLoS OneFebruary 4, 2026Lum Fube, Arul Thason, Elizabeth Taliaferro, Carol Heckman, Surya Amarachintharesolution tiff files. Gene expression by qPCR RNA Isolation. An RNAqueous kit (Invitrogen, cat# AM1912) was used to isolate total RNA from the cells grown in 100 mm culture dishes, plated at a density of

(4) SRSF3 promotes the generation of XBP1s to stabilize autophagy and enhance hypoxia adaptation in gliomaEur J HistochemJanuary 26, 2026Bohu Liu, Xiaoran Zhang, Jintao Tian, Xiaobin Huang, Xuhui Li, Jinxi Zhao, Zhenghu Xu, Jun Puflux was assessed by the GFP/mRFP ratio. RT-qPCR Total RNA was extracted using an RNA isolation kit (AM1912; Invitrogen), and cDNA synthesis and PCR amplification were subsequently performed using a one-step RT-qPCR

(5) Embryo secretome regulation of the endometrial epithelial transcriptome: development of a model to test for embryo viabilityMolecular Human ReproductionJanuary 7, 2026Zhixing Jin, Yifan Wang, Abigail Freeman Blatchford, Helena Rodriguez-Caro, Luyao Wang, Christian M Becker, Pedro Melo, Tim Child, Rebecca Dragovic, Ingrid Granne, et al.isolated from samples using the RNAqueous™-Micro Total RNA Isolation Kit (Thermo Fisher Scientific; AM1912) following the manufacturer’s protocol. RNA quality was assessed using the Agilent 2100 Bioanalyzer

(6) Bronchial epithelial transcriptome reveals dysregulated interferon and inflammatory responses to rhinovirus in exacerbation-prone pediatric asthmaJCI InsightDecember 22, 2025Naresh Doni Jayavelu, Basilin Benson, Patricia C. dela Cruz, Weston T. Powell, Lucille M. Rich, Elizabeth R. Vanderwall, Camile R. Gates, Andrew J. Nagel, Maria P. White, Nyssa B. Samanas, et al.the basolateral chamber of Transwells. Eight hundred microliters of lysis buffer (Invitrogen product AM1912) was added to the apical surface of cultures. A pipette tip was then used to gently scratch each apical

(7) IL-13 Modulates Antiviral Effector and Proinflammatory Pathways in Rhinovirus-Infected Pediatric Bronchial EpitheliumbioRxivDecember 8, 2025Patricia C. Dela Cruz, Basilin Benson, Naresh Doni Jayavelu, Weston T. Powell, Lucille M. Rich, Elizabeth R. Vanderwall, Camile R. Gates, Maria P. White, Nyssa B. Samanas, Kourtnie Whitfield, et al.from BECs in ALI, basolateral media was removed, and 800μL of lysis buffer (Invitrogen®, Product No. AM1912) was added to the apical surface of cultures. To mechanically disrupt BECs from the transwell membrane,

(8) Geographic Divergence and Putative Candidate Gene Discovery of Rice Grain Quality in ChinaRice (N Y)December 1, 2025Fei Wang, Di Cui, Xiaoding Ma, Zichao Zhu, Bing Han, Chunhui Liu, Chutao Wang, Ying Xiong, Chunlin Long, Xianyong Li, et al.at -80 °C. Total RNA was extracted from rice protoplasts using TRIzol reagent (Invitrogen, Cat No. AM1912). RNA (2 µg) was used to prepare first-strand cDNA with TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

(9) Milademetan in Advanced Solid Tumors with MDM2 Amplification and Wild-type TP53: Preclinical and Phase II Clinical Trial ResultsClinical Cancer ResearchOctober 15, 2025Ecaterina E. Dumbrava, Thomas E. Stinchcombe, Mrinal Gounder, Gregory M. Cote, Glenn J. Hanna, Bradley Sumrall, Trisha M. Wise-Draper, Mohammed Kanaan, Steven Duffy, Christopher Sumey, et al.samples. qPCR RNA was extracted using an Invitrogen RNA isolation kit (Thermo Fisher Scientific, cat. No. AM1912), and cDNA were synthesized using a high-capacity cDNA preparation kit (Applied Biosystems; cat. No.

(10) MiR-24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator-Induced Lung Injury by Targeting Bcl-2-related Ovarian KillerPulm CircOctober 7, 2025Wenbo Xu, Wenjiao Ren, Lingling Zhang, Bing Wang, Linqi Gao, Dong YuanRNA Isolation and RT‐qPCR RNA was acquired from rat lung tissues using an RNA Isolation Kit (Cat# AM1912, Thermo Fisher). For mRNA detection, cDNA synthesis was carried out using SuperScript II Reverse Transcriptase

(11) Rare variants in PRKCI cause Van der Woude syndrome and other features of peridermopathyThe American Journal of Human GeneticsOctober 2, 2025Kelsey Robinson, Sunil K. Singh, Rachel B. Walkup, Dorelle V. Fawwal, Kendra M. Vilfort, Amanda Koloskee, Azeez Fashina, Wasiu Lanre Adeyemo, Terri H. Beaty, Azeez Butali, et al.were processed for total RNA extraction using RNAqueous™ Total RNA Isolation Kit (Invitrogen; cat# AM1912). cDNA was prepared from approximately 1µg DNase treated total RNA using High-Capacity cDNA Reverse

(12) Apoptotic bodies in phytoplankton suggest evolutionary conservation of cell death mechanismsNature CommunicationsSeptember 25, 2025Luisa Corredor, Georgia Antonia Vergou, Vladimír Skalický, Ioanna Antoniadi, Benjamin J. Wheaton, Karin Ljung, András Gorzsás, Christiane FunkGene expression analysis RNA extraction Total RNA was extracted using Invitrogen RNAqueous™ Total RNA Isolation Kit (part # AM1912, Thermo Fisher Scientific) with the following modifications: the frozen biomass pellets were mixed with

(13) Precision Targeting of Myeloid Cells via Peptide Dendrimer‐Lipid Nanocarriers: A Novel Platform for Potent Cancer ImmunotherapiesAdvanced ScienceSeptember 19, 2025Lawrence James Billing, Liam Martin, Tristan Henser‐Brownhill, Parisa Samangouei, Adam Leach, Phoebe Knight, Marina Apostolidou, Aaqib Ladak, Benita Nagel, Albert Kwokextracted using the RNAqeous total RNA Isolation kit as per the manufacturer's instructions (ThermoFisher; AM1912). Total RNA samples were subsequently stored in a ‐80 °C freezer prior to submission for RNA‐seq by

(14) MT-125 inhibits non-muscle myosin IIA and IIB and prolongs survival in glioblastomaCellAugust 21, 2025Kenchappa RS, Radnai L, Young EJ, Zarco N, Lin L, Dovas A, Meyer CT, Haddock A, Hall A, Toth K, et al.Pierce™ BCA Protein Assay Kit,Thermo Fisher Scientific,Cat# 23225 RNAqueous™ Total RNA Isolation Kit,Thermo Fisher Scientific,Cat# AM1912 TURBO DNA-free™ Kit,Thermo Fisher Scientific,Cat# AM1907 NEBuilder® HiFi DNA Assembly Master Mix,New England BioLabs

(15) Persistent cortical excitatory neuron dysregulation in adult Chd8 haploinsufficient miceResearchSquareAugust 5, 2025Cesar P. Canales, Stephanie A. Canales, Nicholas A. Lozano, Nicholas Cichewicz, Wellington Cichewicz, Wellington Amaral, Nicholas Fenton, Ayanna Fenton, A Ayanna Wade, Nickolas Smith, et al.Total RNA from these fractions was then extracted using RNA RNAqueous™ Total RNA Isolation Kit (cat# AM1912 ThermoFisher Scientifics). Manufacturer protocols were followed with minor adjustments to increase RNA

(16) Population genomics of a sailing siphonophore reveals genetic structure in the open oceanCurrent BiologyAugust 4, 2025Samuel H. Church, River B. Abedon, Namrata Ahuja, Colin J. Anthony, Dalila Destanović, Diego A. Ramirez, Lourdes M. Rojas, Maria E. Albinsson, Itziar Álvarez Álvarez Trasobares, Reza E. Bergemann, et al.Biotek,Cat#D3373 DNeasy DNA Blood and Tissue kit,Qiagen,Cat#69506 RNAqueous Total RNA Isolation Kit,Invitrogen,Cat#AM1912 RNase I,Invitrogen,Cat#AM2295 RNAse Cocktail Enzyme Mix,Invitrogen,Cat#AM2286 Deposited Data,, PacBio

(17) STAT3 regulates basal cell identity and morphogenesis during early esophageal developmentbioRxiv : the preprint server for biologyAugust 1, 2025Kariuki SW, Mitani Y, Bailey DD, Efe G, Tripathi VV, Tekin H, Suzuki K, Que J, Gabre J, Cruz-Acuña R.analytical algorithms. RT-qPCR RNA was isolated using the RNAqueous Total RNA Isolation Kit (Invitrogen, AM1912), and cDNA was synthesized with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, 43-688-13),

(18) An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis ResearchBIO-PROTOCOLJune 20, 2025Nidhi Bhardwaj, Diksha Rana, Jyotdeep KaurRNeasyTM Mini kit (Qiagen, catalog number: 74104) 10. RNAqueousTM kit (Invitrogen, catalog number: AM1912) 11. Quick-RNATM Miniprep Plus kit (Zymo Research, catalog number: R1057) 12. RNase-free DNase set (Qiagen,

(19) Persistent cortical excitatory neuron dysregulation in adult Chd8 haploinsufficient micebioRxivJune 4, 2025Cesar P. Canales, Stephanie Lozano, Nicholas A. Frost, Karol Cichewicz, Wellington Amaral, Nicolas Seban, Ethan Fenton, Ayanna Wade, Nickolas Chu, Emily Smith, et al.Total RNA from these fractions was then extracted using RNA RNAqueous™ Total RNA Isolation Kit (cat# AM1912 ThermoFisher Scientifics). Manufacturer protocols were followed with minor adjustments to increase RNA

(20) Expression of an interleukin-2 partial agonist enhances regulatory T cell persistence and efficacy in mouse autoimmune modelsNat CommunMay 27, 2025Janie Robert, Manon Feuillolay, María de Temple-Llavero, Reginald Florian Akossi, Vanessa Mhanna, Mustapha Cheraï, Gwladys Fourcade, Frédéric Charlotte, Nicolas Tchitchek, Tian Mi, et al.Subsequently, the cells were lysed for RNA extraction using the RNAqueous Total Isolation Kit (Invitrogen, #AM1912). TRA and TRB libraries were prepared on 100 ng of RNA with the SMARTer Mouse TCR a/b Profiling Kit

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RNAqueous Total RNA Isolation Kit from Thermo Fisher Scientific

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RNAqueous Total RNA Isolation Kit from Thermo Fisher Scientific

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