Description
No need for alcohol precipitation or desalting
The PureLink HQ Mini Plasmid Purification Kit is designed for the isolation of high-quality plasmid DNA that is suitable for restriction enzyme digestion, PCR, sequencing, bacterial cell transformation, and mammalian cell transfection. Using the kit, plasmid DNA can be isolated from varying amounts of bacterial cells.
The PureLink HQ Mini Plasmid Purification Kit has the following advantages:
• Higher spin column capacity for plasmid DNA as compared to other commercially available plasmid purification systems
• Designed to isolate high-quality plasmid DNA in less than an hour
• Minimal genomic DNA contamination of the purified sample
• Reliable performance of the purified DNA in downstream applications
Process overview
Bacterial cells are pelleted, resuspended, and lysed. The lysate is then neutralized and conditions are adjusted for subsequent binding. After clarification by centrifugation, the lysate is processed through the PureLink spin column. The DNA binds to the silica-based membrane in the column, and impurities are removed by a single wash step. The DNA is then eluted in Elution Buffer or water