Human IL-6 Quantikine ELISA Kit D6050B from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IL-6 Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberD6050B
Quantity1 Kit (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range3.13 - 300 pg/mL
ReactivityHuman
Sample TypeCell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity0.626 pg/mL
Detection TargetIL-6
Assay Time4.5 hour
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nameinterleukin 6
NCBI Gene AliasesBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mechanistic Insights Into UC-MSC Therapy in a BAC-Induced Dry Eye Model: TSG-6-Dependent Inhibition of the Th17/CCL20-CCR6 Chemotactic AxisInvest Ophthalmol Vis SciApril 27, 2026Qi Liang, Zeying Chen, Yaoyao Liu, Rongjie Guo, Di Zhang, Jiaxuan Jiang, Chenchen Wang, Kai HuAbcam, USA; Human IL-17A ELISA kit, ml058052; Enzyme-linked Biotechnology, China; Human IL-6 ELISA kit, D6050B; Bio-Techne, China; and Mouse CCL20 ELISA kit, MCC200; Bio-Techne, China) were used. The final concentrations

(2) The Relationship Between Decreased Serum Klotho Protein and Cognitive Impairment in Patients After Abdominal SurgeryJ Inflamm ResApril 17, 2026Xuehui Li, Feng Aowere purchased from Solarbio (Beijing, China), while IL-1β (catalog no. DLB50) and IL-6 (catalog no. D6050B) ELISA kits were obtained from R&D Systems (Minneapolis). Briefly, serum samples were collected, allowed

(3) Rejuvenation of mesenchymal stromal cells via partial reprogramming enables scalable generation of transcriptionally diverse MSC librariesStem Cell Research & TherapyMarch 20, 2026Haochen Tu, Aoi Hosaka, Genki Hichiwa, Yayan Wang, Kanako Kazuki, Toshiaki Tabata, Mitsuhiko Osaki, Yuji Nakayama, Iori Kanazawa, Kazuhisa Honma, et al.(HGF) and interleukin-6 (IL-6) in the supernatants were measured using ELISA kits DHG00B (HGF) and D6050B (IL-6) (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s instructions. Indoleamine

(4) Soluble factors released from fibroblast-like synoviocytes of JIA patients with future extended course can condition persistent JIA synovial fibroblasts for increased IL-6 productionArthritis Research & TherapyMarch 7, 2026Megan M. Simonds, Lina Al-Jaberi, Hermine I. Brunner, AnneMarie C. Brescia[20, 23–26, 29] except they were performed using the following assay purchased from R&D Systems: IL-6 (D6050B). All ELISAs were performed according to manufacturer’s protocols for sample dilution, technical replicates,

(5) Cadherin-11 integrates Piezo1 and interleukin-6 signaling to promote fibroblast activationBiomaterials ScienceMarch 3, 2026Leilani R. Astrab, Steven R. Caliarinitrogen and stored at −70 °C until IL-6 quantification using a human IL-6 ELISA Kit (R&D Systems, D6050B). All samples and standards were run in duplicate according to manufacturer's instructions and fluorescence

(6) Klebsiella pneumoniae infection induces an S100A8/A9-mediated autocrine loop in human airway epithelium to amplify inflammationFront MicrobiolMarch 3, 2026Jie Zheng, Xiuchan Feng, Qianrui Zeng, Shutong Chen, Caihong Yan, Zhijia Huangquantified by commercial Quantikine ELISA kits (R&D Systems, Cat# DS8900 for S100A8/A9, D8000C for IL-8, and D6050B for IL-6). Following the kit instructions, absorbance was measured (F50, TECAN). 2.7 siRNA-mediated

(7) LYVE-1 identifies asthma and drives PDGF-BB-induced proliferation, migration, and oxidative stress in airway smooth muscle cells via the PI3K/Akt pathwayFrontiers in PharmacologyFebruary 11, 2026Yunbo Tang, Nan Zhou, Xiaohui Nialiquoted, and stored at −80 °C until analysis. Secreted levels of TNF-α (DTA00D), IL-1β (DLB50), and IL-6 (D6050B) were quantified using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) according

(8) IL-6R blockade with tocilizumab disrupts pericyte- and tumor cell-driven IL-6/STAT3 signaling, enhancing docetaxel efficacy in ER+ breast cancerbioRxivJanuary 31, 2026Roza Kamila Przanowska, Jacky Gomez-Villa, Victoria J. Liu, Natalia Antonides-Jensen, Lavanya Visvabharathy, John C. Alverdy, Sonia L. Hernandez, Samantha S. Yeeutilized fresh/immediately for running the ELISA using the Human IL-6 ELISA kit – Quantikine (Bio-Techne #D6050B) as per the manufacturer’s instructions. No freeze-thaw cycles occurred previously for these samples.

(9) Skin Barrier Enhancement and Moisturizing Effects of Exosome Extracts Derived from Pinus densiflora, Zanthoxylum piperitum, and Lagerstroemia indica PlantsBiology (Basel)January 29, 2026Ha-Rim Kim, Seung-Hyeon Lee, Won Been Bae, Min-Ji Shin, Seon-Young Kim, Youn Ok Jung, Mi Hee Parkevaluate skin-moisturizing efficacy. Inflammatory cytokine IL-6 was quantified using ELISA kits (cat# D6050B; R&D systems, Minneapolis, UK). Biomarkers for moisturizing activity, including type I procollagen (cat#

(10) Cold Atmospheric Plasma Promotes Anti-Inflammatory and Regenerative Responses in Oral Soft Tissue through Redox-Driven Mitochondrial RegulationACS OmegaJanuary 20, 2026Tien Ngoc Thuy Nguyen, Byung-Hoon Kim(ELISA) kits from R&D Systems (Minneapolis, MN, USA) were used to quantify cytokine production. IL-6 (D6050B), IL-1β (DLB50), and TNF-α (DTA00D) concentrations were measured in cell culture supernatants collected

(11) From multi-omics to functional validation: the PTMRS stratifies TME and positions PDGFRB in CRC biologyFrontiers in ImmunologyJanuary 12, 2026Yanhong Liu, Chao Lu, Luchun Hua, Hankun Hao, Qijing Zhang, Zongyou Zou, Jianbin Xiang, Yaping Wangenzyme-linked immunosorbent assay (ELISA) kits from R&D Systems (CCL5, Catalog #DRN00B; CXCL12, Catalog #DSA00; IL-6, Catalog #D6050B; IL-10, Catalog #D1000B; TGF-β1, Catalog #DB100C).Recombinant cytokine standards provided with each

(12) Bioactive compounds from Sargassum horneri attenuates inflammation and obesity regulating by Nrf2/HO-1 and AMPK signaling pathwaysSci RepDecember 10, 2025Ramakrishna Chilakala, Hyeon Jeong Moon, Min Ju Kim, Kang Ho Ko, Jong Won Han, Min Seouk Jung, Sun Hee CheongMillipore (Billerica, MA, USA). To measure the levels of PGE2 (#KGE004B), TNF-α (#MTA00B), and IL-6 (#D6050B), we used enzyme-linked immunosorbent assay (ELISA) kits, which were produced from R&D Systems, Inc.

(13) Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3 / mTORC1 Signalling in ALS MiceImmunologyNovember 6, 2025Jie Han, Xi Xu, Yan Zhao, Yao Xiao, Fei Huang, Jing Zhou, Hai Huang, Guiqian Wangat 4°C to isolate serum. Mouse‐specific ELISA kits (M4000B, M1000B, DB100C, QK285, MTA00B, MLB00C, D6050B; R&D Systems, USA) were used according to the manufacturer's instructions. For each assay, 50 μL of

(14) Phytochemical Profiling, Anti-Inflammatory Action, and Human Gut Microbiota-Assisted Digestion of Rheum officinale Petiole and Root Extracts—An In Vitro StudyNutrientsNovember 1, 2025Oleksandra Liudvytska, Mariusz Kowalczyk, Justyna Krzyżanowska-Kowalczyk, Karolina Michaś, Maria Michalak, Aneta Balcerczyk, Weronika Skowrońska, Marcin Równicki, Agnieszka Bazylko, Monika A. Olszewska, et al.(R&D Systems, Minneapolis, MN, USA), i.e., TNF-α—Catalog #: DTA00D; IL-2 Catalog #: DT2050; IL-6 Catalog #: D6050B, respectively. 2.8. Cytotoxicity Assays in PBMCs Culture The examined extracts were added to the PBMCs

(15) Serum levels of EphA2 are elevated in knee osteoarthritis and associated with disease severityFront Med (Lausanne)October 8, 2025Maping Xiao, Lei Ji, Shuang Feng, Wenbin QianEK-Bioscience), aggrecan (DY1220, R&D Systems), TNF-α (DTA00D, R&D Systems), IL-1β (DLB50, R&D Systems), IL-6 (D6050B, R&D Systems), IL-17A (D1700, R&D Systems), MDA (EU2577, FineTest), GSH (ml038257, Shanghai Enzyme-linked

(16) Cadherin-11 integrates Piezo1 and interleukin-6 signaling to promote fibroblast activationbioRxivSeptember 22, 2025Leilani R. Astrab, Steven R. Caliariliquid nitrogen and stored at −70°C until IL-6 quantification using a human IL-6 ELISA Kit (R&D Systems, D6050B). All samples and standards were run in duplicate according to manufacturer’s instructions and fluorescence

(17) Elevated Serum Podoplanin Levels Correlate with Disease Severity and Inflammation in Ulcerative Colitis: A Cross-Sectional StudyJournal of Inflammation ResearchSeptember 18, 2025Xiaogang Huang, Caifang Tangmeasured using ELISA kits for Interleukin-1 beta (IL-1β; DLB50, R&D Systems), Interleukin-6 (IL-6; D6050B, R&D Systems), Interleukin-17A (IL-17A; D1700, R&D Systems), Tumor necrosis factor-alpha (TNF-α; DTA00D,

(18) Baricitinib and Infliximab Mitigate the Endothelial-to-Mesenchymal Transition (EndMT) Induced by Cytokines in HUVECsInternational journal of molecular sciencesSeptember 5, 2025Barilli A, Visigalli R, Recchia Luciani G, Crescini E, Dall'Asta V, Rotoli BM.

(19) Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathwayPhysiological reportsSeptember 1, 2025Xu Y, Li W, Shi J, Guo Y.interleukin‐1 beta (IL‐1β) (Cat#DLB50, R&D Systems, Minneapolis, USA), and interleukin‐6 (IL‐6) (Cat#D6050B, R&D Systems, Minneapolis, USA) were assessed using enzyme‐linked immunosorbent assay (ELISA) kits (R&D

(20) Network Pharmacology and Experiment Verification-Based Strategy for Exploring the Mechanisms of Shuqing Granule in the Treatment of COVID-19Pharmaceuticals (Basel, Switzerland)August 18, 2025Guo X, Zheng H, An Y, Song Y, Liu T, Zhou Z, Liu C, Wang G, Wang F.Enzyme-Linked Immunosorbent Assays (ELISAs) Human and mouse IL-6 ELISA kits were purchased from R&D Systems (Catalog Number: D6050B, M6000B-1, Minneapolis, MN, USA). Cell culture supernatants and mouse sera from each group were collected

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Human IL-6 Quantikine ELISA Kit

IL-6 ELISA

Human IL-6 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Human IL-6 Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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