Ultra High Sensitivity ECL Kit HY-K1005 from MedChemExpress

Product Specs
ItemUltra High Sensitivity ECL Kit
CompanyMedChemExpress
Price $77.00Supplier Page View Company Product Page
Catalog NumberHY-K1005
Quantity100 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Forsythoside A Alleviates Neuroinflammatory Damage via Inhibiting TLR4/NF-κB/NLRP3 Activation-Induced Astrocyte Pyroptosis in Cerebral Ischemia-ReperfusionCNS Neurosci TherApril 8, 2026Yupeng Guo, Xuanwei Dong, Min Liu, Jianxin Wang, Shewei Guowas exposed to goat anti‐rabbit IgG (ab97051, 1:2000, Abcam) at 25°C for 2 h. ECL working solution (HY‐K1005, MedChemExpress) was prepared and evenly dropped on the membrane, which was then imaged using an iBright

(2) Pre-differentiation neurotoxicity of GenX exposure on hiPSC-derived cortical neuronsEnvironmental Science & TechnologyApril 7, 2026Shichen Wu, Junkai Xie, Xihui Zhao, Han Zhao, Oscar F. Sánchez, Serena Yu, Jean-Christophe Rochet, Jennifer L. Freeman, Chongli Yuanantibody. Signals were developed in enhanced chemiluminescence substrates solution (MedChemExpress, HY-K1005) and visualized using a ChemiDoc Imaging system (Bio-Rad). Quantification was performed using ImageJ

(3) Comparative neurotoxic effects of PFOA and GenX in hiPSC-derived cortical neuronsJournal of Hazardous MaterialsApril 1, 2026Han Zhao, Junkai Xie, Shichen Wu, Xihui Zhao, Serena Yu, Jennifer L. Freeman, Jean-Christophe Rochet, Chongli Yuanbuffer for 10 min three times before incubation with enhanced chemiluminescence (ECL) substrates (MCE, HY-K1005). Blots were imaged using a ChemiDoc Imaging System (Bio-Rad). 2.13 Data analysis and statistics Differentiated

(4) Retinoic acid inhibits the infection of porcine reproductive and respiratory syndrome virusFrontiers in Veterinary ScienceMarch 24, 2026Jintong Guo, Shuangyi Xiehe, Mengmei Wang, Tianle Li, Qiyue Zheng, Ahui Wang, Shengcan Xie, Wenjing Wan, Yiyu Zhao, Wei Ma, et al.temperature for 1 h. Signals were detected using an Ultra High Sensitivity ECL Kit (MedChemExpress, HY-K1005, Monmouth Junction, NJ) and visualized with ChampChemi i610Plus. All immunoblots were performed in three

(5) Integrated Multi-Omics Analysis Reveals IRF1-Driven Microglial PANoptosis via ZBP1 in Spinal Cord InjuryJ Inflamm ResMarch 16, 2026Xiongjie Xu, Song Chen, Shichao Liu, Yinqing Wang, Chunhua Wang, Risheng Liang1:10000) for 1 h at room temperature. Protein bands were visualized using ultrasensitive ECL substrate (HY-K1005, MedChemExpress). β-actin or Vinculin were respectively used as the loading control in the same experiment. Immunofluorescence Immunofluorescence

(6) Propofol upregulates MFG‑E8 in BV2 cells to inhibit pyroptosis mediated by the NF‑κB/NLRP3 pathway, thereby ameliorating ischemic‑reperfusion neuronal injuryInt J Mol MedMarch 5, 2026Shewei Guo, Yingwei Zhen, Guosheng Zhou, Zhihua ZhaoInvitrogen; Thermo Fisher Scientific, Inc.) for 2 h at 25°C. Subsequently, the ECL working solution (cat. no. HY-K1005, MedChemExpress) was prepared and uniformly dropped onto the membrane, which was scanned using iBright

(7) Long non-coding RNA LINC00607 epigenetically regulates endothelial TSPAN18 to promote hypoxia-induced thromboinflammationJournal of Biological ChemistryJanuary 23, 2026MOHD YASIR KHAN, KASHIKA SINGH, ALIA HASHMI, ARMIYA SULTAN, KHAN SADIA, MOHAMMAD ZAHID ASHRAFwere washed again three times in 0.1% PBST and developed using enhanced chemiluminescence (ECL; MCE; HY-K1005) detection reagent. Protein bands were visualized using a CCD-based imaging system (Biorad), and band

(8) NLRC5 Regulates Enterovirus 71 Infection Through an IFN-β-Dependent PathwayVirusesJanuary 23, 2026Wei Fang, Binbin Zhu, Tan Ge, Xuejuan Liu, Bao Li, Baojing LuChina) for 1 h at room temperature. Following another wash with TBST, an-ultra-high sensitivity ECL (HY-K1005, MCE, Monmouth Junction, NJ, USA) was added to visualize the target proteins. 2.4. Real-Time Quantitative PCR

(9) Aged skin exacerbates experimental osteoarthritis via enhanced IL-36R signalingNature CommunicationsJanuary 14, 2026Dalin Chen, Chong Wang, Changsheng Yang, Qinwei Cheng, Liangyu Chen, Panpan Yang, Zilin Zou, Zining Wang, Honghao Li, Yun Xiao, et al.chemiluminescence imager (Tanon, Model 5200CE) using Ultra High Sensitivity ECL Kit (MedChemExpress, Cat No. HY-K1005). Detailed information regarding the primary and secondary antibodies used for Western blot analysis

(10) Targeting the FOSL1/IKKα positive feedback loop attenuates glioblastoma malignancy via suppression of NF-κB signalingTheranosticsJanuary 1, 2026Beichen Zhang, Hao Tian, Haoyu Zhou, Yichang Wang, Ke Gao, Yiyang Cao, Mingjing Zhou, Maode Wang, Wei Wu, Jia Wangfor 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL Kit (HY-K1005, MCE), and chemiluminescence signals were captured using a digital imaging system. A complete list of

(11) Multi-omics analysis reveals shared diagnostic and therapeutic targets in endometriosis and recurrent implantation failureSci RepDecember 29, 2025Jie Yu, Wei Wang, Qiong Li, Lan Lan, Li-Li Jiang, Xin-Rong He, Xiao-Wei Jiang, Yu-Lin Yan, Xiao-Ming Yao, Meng-Yue Wang, et al.1 h. Protein signals were visualized using an enhanced chemiluminescence (ECL) detection kit (MCE; HY-K1005). The grayscale values were analyzed using ImageJ software (1.53T, National Institutes of Health (NIH),

(12) Sleep fragmentation exacerbates myocardial ischemia-reperfusion injury via hypothalamic paraventricular nucleus-resident OX1R-mediated sympathetic hyperactivity in adult miceAnn MedDecember 10, 2025Xiaocheng Zhu, Lizhe Guo, Na Chen, Lu Wang, Sisi Dai, Xingyang Liu, Xiang Chen, Caiyi Wang, Yanan Cao, Hao Hu, et al.(H + L) (1:10000, Jackson, 111-035-144). The protein bands were developed with the ECL reagent (MCE, HY-K1005). Protein bands were acquired using the GE IMAGEQUANTMT 800 CCD imager and quantified using the ImageJ

(13) Identification of novel PTP1B inhibitor for the treatment of LPS-induced myocardial apoptosis: machine learning based virtual screening and biological evaluationJ Enzyme Inhib Med ChemDecember 10, 2025Xu Dong, Bing Shang, Xin Li, Jie Zhang, Zhen Liu, Bo Fengantibodies and secondary antibodies subsequently. Bands were visualised by ECL chemiluminescence kit (MCE, HY-K1005) and quantified by ImageJ software. Statistical analysis Statistical analysis was performed by GraphPad

(14) Cholesterol induced-mitochondrial calcium dysregulation facilitates atherosclerosis by promoting lipid accumulation in vascular smooth muscle cellsMol BiomedDecember 2, 2025Zhiwang Zhang, Fan Yang, Wei Wang, Qi Cao, Long Zhang, Yu Zhang, Dong Ma, Xinhua Zhang, Jinkun Wen, Bin Zhengantibody for 1 h at room temperature. The protein expression was detected using an ECL detection kit (HY-K1005, MedChemExpress, USA) and normalized using the β-actin levels [32]. For co-immunoprecipitation, A/G

(15) The Strontium Ion Reprograms Monocyte Subsets via TRPM2 Channel Regulation to Enhance OsseointegrationBiomaterials ResearchNovember 24, 2025Congrui Zhao, Antian Xu, Jingyao Gong, Yangbo Xu, Ping Sun, Fuming Hetemperature for 1 h. The immunoreactive bands were developed with enhanced chemiluminescence solution (MCE, HY-K1005) and visualized by a chemiluminescent imager. Immunofluorescent staining Monocytes were seeded into

(16) Comparative study of rebaudioside A and rebaudioside Aα: physicochemical properties and in vivo metabolismnpj Science of FoodNovember 14, 2025Hye-Jin Kim, Hye-Jin Kang, Sangyi Lim, Ok Cheol Kim, Ki-Nam Yoon, Junhyeong Lee, Merc Emil Matienzo, Min-Jung Park, Bu-Soo Park, Jeong-Yong Cho, et al.antibodies. To detect proteins, enhanced chemiluminescence solutions (#EOE001S; Enzynomics, Daejeon, Korea; HY-K1005; MedChemExpress, Suite Q Monmouth Junction, NJ, USA) were used. This study utilized antibodies against

(17) Identifying SUMOylation-related genes in liver fibrosis with bioinformatics and experimental models for diagnostic insightsScientific ReportsNovember 13, 2025Zhiwei Su, Yuxue Ding, Juan Xue, Jun Sun, Chunyan JiThe bands’ grey values were captured, photographed, and analyzed using ECL Chemiluminescent Reagent (HY-K1005, MedChemExpress). Immunohistochemical assessments We prepared liver tissue samples into 4 μm paraffin-embedded

(18) SIK2-mediated phosphorylation of GABARAPL2 facilitates autophagosome-lysosome fusion and rescues neurodegeneration in an Alzheimer's disease modelTransl NeurodegenerOctober 23, 2025Xiaoman Dai, Ziling Ye, Chen Wang, Yufei Huang, Yun Chen, Tianqing Han, Weijie Gao, Xin Wu, Jing Zhang, Xiaochun Chenroom temperature (RT). Signals were detected using an Ultra High Sensitivity ECL Kit (MedChemExpress, HY-K1005, Monmouth Junction, NJ) and visualized using an imaging system. Target band intensities were quantified

(19) Dimethyl fumarate reprograms cervical cancer cells to enhance antitumor immunity by activating mtDNA-cGAS-STING pathwayJ Biomed SciOctober 20, 2025Han Jiang, Liting Liu, Shan He, Shen Qu, Yifan Yang, Guijie Kang, Min Wu, Hangyu Liu, Yuwei Zhang, Zixuan Wang, et al.h at room temperature, washed five times with TBST again, and protein bands were visualized by ECL (HY-K1005, MCE). Quantitative analysis of protein expression was performed using ImageJ (v.1.52). Measurement

(20) Natural Small-Molecule Bergapten Ameliorates Amyloid-β Pathology and Neuroinflammation in Alzheimer's DiseaseNutrientsOctober 14, 2025Jingyan Zhang, Jing Zhangantibodies. Immunoreactive proteins were detected using an enhanced chemiluminescent (ECL) substrate (MCE, HY-K1005) and visualized with a Chemiluminescence Imager (eBLOT, Tanon, Xiamen, China). The primary antibodies

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Ultra High Sensitivity ECL Kit from MedChemExpress

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Ultra High Sensitivity ECL Kit from MedChemExpress

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