Ultra High Sensitivity ECL Kit HY-K1005 from MedChemExpress

Product Specs
ItemUltra High Sensitivity ECL Kit
CompanyMedChemExpress
Price $77.00Supplier Page View Company Product Page
Catalog NumberHY-K1005
Quantity100 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Down-regulation of THBS2 inhibits the malignant progression of Burkitt lymphoma by blocking the PI3K/AKT/c-MYC pathwayFront OncolJuly 24, 2026Yahong Xu, Shengquan Liu, Shaoxiong Wang, Yuanling Huangovernight, and then incubated with the corresponding secondary antibody dilution for 2 h. ECL kit (HY-K1005, MedChemExpress) was used for chemiluminescence detection, and gel imaging system (Geldoc Go, Bio-Rad,

(2) Case Report: Novel homozygous pathogenic variant of the SPG20 gene causes the Troyer syndrome in ChinaFront GenetJuly 2, 2026Lina Zhu, Siqi Hu, Xinyang Jiang, Rujie Gu, Yongxia Wang, Shufang Zhang, Fujun Peng, Xiuwei Magoat anti-rabbit or anti-mouse secondary antibodies. Protein bands were visualized using an ECL kit (HY-K1005, MCE) on a ChemiDoc Imaging System (Bio-Rad). Immunofluorescence microscopy For immunofluorescence,

(3) Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibitionFront NeurolJune 3, 2026Ni Wang, Wei Guo, Xiaochen Yu, Yaqing Sun, Shujing Shi, Yan Xie(HRP), ab6721, 1:2000, Abcam] under the transition condition (37 °C, 2 h). The ECL luminescent solution (HY-K1005, MedChemExpress) was used to enhance the fluorescence signal, and the gel imaging system (iBright CL1500,

(4) Pre-Activation of Mitophagy Protects Against Hyperbaric Oxygen-Induced Central Nervous System Oxygen ToxicityInt J Mol SciMay 30, 2026Wei Ding, Qi Zhu, Houyu Zhao, Guanning Wei, Guoyang Huang, Jing Shi, Longfei Wang, Nan Zhao, Jin Ding, Yiqun Fangsignals were visualized using enhanced chemiluminescence (MCE (South Brunswick Township, NJ, USA), HY-K1005) and quantified using ImageJ software (v1.54f). 4.11. Immunofluorescence and Confocal Microscopy Immunofluorescence

(5) Sintilimab plus concurrent chemoradiotherapy for treatment of locally advanced small cell lung cancer (SINCE-01): a phase II clinical trialSignal Transduct Target TherMay 29, 2026Qian Zhao, Yintao Li, Hongjiao Zhang, Dan Han, Yanni Kan, Hongfu Sun, Chengxin Liu, Linlin Wang, Xiao Han, Ying Hua, et al.with HRP-conjugated secondary antibodies and visualized using enhanced chemiluminescence substrate (HY-K1005, MCE). RNA extraction and gene expression analysis Total RNA was extracted using the SPARKeasy Cell

(6) Astragaloside IV inhibits nasopharyngeal carcinoma progression by inhibiting SATB2/Wnt/PD-L1 pathway and enhancing the killing activity of T cellsEuropean Journal of HistochemistryMay 28, 2026Yinping Zeng, Jiajun Huang, Tingting Duan, Xiaofeng Wangcondition (37°C, 2 h) was continued to incubate the secondary antibody. The ECL luminescent liquid (HY-K1005, MedChemExpress) was used to enhance the fluorescence signal, and the gel imaging system (iBright CL1500,

(7) Loss of KLF15 expression characterizes proximal tubule injury in cisplatin-induced acute kidney injury: A multi-omics studyCurrent Research in ToxicologyMay 19, 2026Lei Chen, Shiying Xie, Heqing Yang, Xiangshen Liu, Shunliang Yang, Dong Wangfor 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL Kit (HY-K1005, MCE, USA) and quantified with ImageJ software (NIH, Bethesda, MD, USA). A detailed list of antibodies

(8) Elevated TRIM25 Impairs Poly (ADP‐ribose) Metabolism via PARG Degradation and Mediates Compression‐Induced Intervertebral Disc DegenerationAdvanced ScienceMay 1, 2026Zhangrong Cheng, Haiyang Gao, Wenbo Wu, Pengzhi Shi, Xianglong Chen, Zimu Yu, Wang Wu, Kangcheng Zhao, Cao Yang, Yukun Zhangfor 1 h at room temperature. Protein bands were visualized using enhanced chemiluminescence reagent (#HY‐K1005, MedChemExpress, USA) on a ChemiDoc MP Imaging System (Bio‐Rad, USA), and band intensity was quantified

(9) Design of Safe and Efficient Adenine Base Editors via Protein Language Model Screening for Osteoarthritis TreatmentAdvanced ScienceMay 1, 2026Jiawei Yao, Dalin Chen, Ziyi Zhang, Jingxuan Ren, Chengcheng Zhao, Shengfang Wang, Mengyu Shang, Dawei Jiang, Yinuo Li, Su'an Tang, et al.temperature. Protein bands were visualized using an Ultra High Sensitivity ECL Kit (MedChemExpress, Cat. No. HY‐K1005) and captured with a chemiluminescence imaging system (Tanon, Model 5200CE). 4.24 RNA Extraction and

(10) Forsythoside A Alleviates Neuroinflammatory Damage via Inhibiting TLR4/NF-κB/NLRP3 Activation-Induced Astrocyte Pyroptosis in Cerebral Ischemia-ReperfusionCNS Neurosci TherApril 8, 2026Yupeng Guo, Xuanwei Dong, Min Liu, Jianxin Wang, Shewei Guowas exposed to goat anti‐rabbit IgG (ab97051, 1:2000, Abcam) at 25°C for 2 h. ECL working solution (HY‐K1005, MedChemExpress) was prepared and evenly dropped on the membrane, which was then imaged using an iBright

(11) Pre-differentiation neurotoxicity of GenX exposure on hiPSC-derived cortical neuronsEnvironmental Science & TechnologyApril 7, 2026Shichen Wu, Junkai Xie, Xihui Zhao, Han Zhao, Oscar F. Sánchez, Serena Yu, Jean-Christophe Rochet, Jennifer L. Freeman, Chongli Yuanantibody. Signals were developed in enhanced chemiluminescence substrates solution (MedChemExpress, HY-K1005) and visualized using a ChemiDoc Imaging system (Bio-Rad). Quantification was performed using ImageJ

(12) Comparative neurotoxic effects of PFOA and GenX in hiPSC-derived cortical neuronsJournal of Hazardous MaterialsApril 1, 2026Han Zhao, Junkai Xie, Shichen Wu, Xihui Zhao, Serena Yu, Jennifer L. Freeman, Jean-Christophe Rochet, Chongli Yuanbuffer for 10 min three times before incubation with enhanced chemiluminescence (ECL) substrates (MCE, HY-K1005). Blots were imaged using a ChemiDoc Imaging System (Bio-Rad). 2.13 Data analysis and statistics Differentiated

(13) Retinoic acid inhibits the infection of porcine reproductive and respiratory syndrome virusFrontiers in Veterinary ScienceMarch 24, 2026Jintong Guo, Shuangyi Xiehe, Mengmei Wang, Tianle Li, Qiyue Zheng, Ahui Wang, Shengcan Xie, Wenjing Wan, Yiyu Zhao, Wei Ma, et al.temperature for 1 h. Signals were detected using an Ultra High Sensitivity ECL Kit (MedChemExpress, HY-K1005, Monmouth Junction, NJ) and visualized with ChampChemi i610Plus. All immunoblots were performed in three

(14) Integrated Multi-Omics Analysis Reveals IRF1-Driven Microglial PANoptosis via ZBP1 in Spinal Cord InjuryJ Inflamm ResMarch 16, 2026Xiongjie Xu, Song Chen, Shichao Liu, Yinqing Wang, Chunhua Wang, Risheng Liang1:10000) for 1 h at room temperature. Protein bands were visualized using ultrasensitive ECL substrate (HY-K1005, MedChemExpress). β-actin or Vinculin were respectively used as the loading control in the same experiment. Immunofluorescence Immunofluorescence

(15) Integrated Multi-Omics Analysis Reveals IRF1-Driven Microglial PANoptosis via ZBP1 in Spinal Cord InjuryJ Inflamm ResMarch 16, 2026Xiongjie Xu, Song Chen, Shichao Liu, Yinqing Wang, Chunhua Wang, Risheng Liang1:10000) for 1 h at room temperature. Protein bands were visualized using ultrasensitive ECL substrate (HY-K1005, MedChemExpress). β-actin or Vinculin were respectively used as the loading control in the same experiment. Immunofluorescence Immunofluorescence

(16) Propofol upregulates MFG‑E8 in BV2 cells to inhibit pyroptosis mediated by the NF‑κB/NLRP3 pathway, thereby ameliorating ischemic‑reperfusion neuronal injuryInt J Mol MedMarch 5, 2026Shewei Guo, Yingwei Zhen, Guosheng Zhou, Zhihua ZhaoInvitrogen; Thermo Fisher Scientific, Inc.) for 2 h at 25°C. Subsequently, the ECL working solution (cat. no. HY-K1005, MedChemExpress) was prepared and uniformly dropped onto the membrane, which was scanned using iBright

(17) Long non-coding RNA LINC00607 epigenetically regulates endothelial TSPAN18 to promote hypoxia-induced thromboinflammationJournal of Biological ChemistryJanuary 23, 2026MOHD YASIR KHAN, KASHIKA SINGH, ALIA HASHMI, ARMIYA SULTAN, KHAN SADIA, MOHAMMAD ZAHID ASHRAFwere washed again three times in 0.1% PBST and developed using enhanced chemiluminescence (ECL; MCE; HY-K1005) detection reagent. Protein bands were visualized using a CCD-based imaging system (Biorad), and band

(18) NLRC5 Regulates Enterovirus 71 Infection Through an IFN-β-Dependent PathwayVirusesJanuary 23, 2026Wei Fang, Binbin Zhu, Tan Ge, Xuejuan Liu, Bao Li, Baojing LuChina) for 1 h at room temperature. Following another wash with TBST, an-ultra-high sensitivity ECL (HY-K1005, MCE, Monmouth Junction, NJ, USA) was added to visualize the target proteins. 2.4. Real-Time Quantitative PCR

(19) Aged skin exacerbates experimental osteoarthritis via enhanced IL-36R signalingNature CommunicationsJanuary 14, 2026Dalin Chen, Chong Wang, Changsheng Yang, Qinwei Cheng, Liangyu Chen, Panpan Yang, Zilin Zou, Zining Wang, Honghao Li, Yun Xiao, et al.chemiluminescence imager (Tanon, Model 5200CE) using Ultra High Sensitivity ECL Kit (MedChemExpress, Cat No. HY-K1005). Detailed information regarding the primary and secondary antibodies used for Western blot analysis

(20) Targeting the FOSL1/IKKα positive feedback loop attenuates glioblastoma malignancy via suppression of NF-κB signalingTheranosticsJanuary 1, 2026Beichen Zhang, Hao Tian, Haoyu Zhou, Yichang Wang, Ke Gao, Yiyang Cao, Mingjing Zhou, Maode Wang, Wei Wu, Jia Wangfor 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL Kit (HY-K1005, MCE), and chemiluminescence signals were captured using a digital imaging system. A complete list of

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Ultra High Sensitivity ECL Kit from MedChemExpress

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Ultra High Sensitivity ECL Kit from MedChemExpress

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