JC-1 Mitochondrial Membrane Potential Assay Kit HY-K0601 from MedChemExpress

Product Specs
ItemJC-1 Mitochondrial Membrane Potential Assay Kit
CompanyMedChemExpress
Price $95.00Supplier Page View Company Product Page
Catalog NumberHY-K0601
Quantity100 T
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Multi-targeted biomimetic CeO₂-based nanoplatform for sepsis therapy via mitochondrial homeostasisInternational Journal of Pharmaceutics: XDecember 1, 2026Xiaolin Tan, Zaili Zhang, Mingzhe Wu, Yuan Tan, Xue Zhang, Chang Li, Zhifeng Wen, Yanxia Lin, Jun Chen, Yao Li(ΔΨm) ΔΨm was assessed using the JC-1 fluorescent dye. RAW264.7 cells were incubated with JC-1 (5 μg/ml, HY-K0601, MCE) for 15 min at 37 °C. Cells were then imaged, where green fluorescence (∼535 nm, JC-1 monomers)

(2) Design, Synthesis, and Evaluation of Novel Adenine-Based Hydrazones as Potential Anticancer Agents: Cytotoxicity Against A549 and MRC-5 Cell Lines with In Silico InsightsPharmaceuticals (Basel)October 19, 2026Emre Menteşe, Nedime Çalışkan, Mustafa Emirik, Adem GünerMitochondrial Membrane Potential (MMP, ΔΨm) Mitochondrial membrane potential was examined using JC-1 dye (Cat. No. HY-K0601, MedChemExpress, Monmouth Junction, NJ, USA). A549 cells were seeded in 96-well plates at 1 × 105 cells/well

(3) Fluoropyrimidine-associated gastrointestinal toxicity: pharmacovigilance reporting signals and in vitro evidence of epithelial injury, barrier impairment, and inflammatory responsesFrontiers in ImmunologySeptember 29, 2026Xinchi Luan, Xiaoran Li, Xiaoxuan Li, Xuezhe Wang, Xiaolei Zhang, Bangshen Zhao, Jia Xu, Zhanghao Yu, Guo Jing, Ning Liuwas evaluated using a JC-1 Mitochondrial Membrane Potential Assay Kit (MedChemExpress, catalog no. HY-K0601). Red and green fluorescence signals were measured at excitation/emission wavelengths of 510/527 nm

(4) RBM15 affects mitophagy in intracerebral hemorrhage by promoting E2F1 nuclear translocation via NXF1Frontiers in ImmunologySeptember 16, 2026Jiwei Sun, Haoyu Yin, Jian Song, Ning Li, Jie Feng, Xuefeng Lu, Yi Han, Yesen Zhangfluorescence microscope. JC-1 staining HT22 cells: Samples were incubated with JC-1 working solution (MCE, HY-K0601) in a dark environment at 37 °C for 30 min. After washing three times with PBS, the samples were incubated

(5) Serum from Patients with Antiphospholipid Syndrome Differentially Affects Venous and Microcirculatory Endothelial CellsBiology (Basel)August 27, 2026Daniele Mancardi, Francesco Maximillian Anthony Shelton Agar, Pasquale Pagliaro, Clara Marinotti, Elisa Arrigo, Alice Barinotti, Irene Cecchi, Massimo Radin, Silvia Grazietta Foddai, Dario Roccatello, et al.mitochondrial membrane potential was assessed using a JC-1 Mitochondrial Membrane Potential Assay Kit (HY-K0601, MedChemExpress LLC, Monmouth Junction, NJ, USA). For depolarization controls, CCCP was added 10 min

(6) A Multifunctional Chitosan-Coated Ciprofloxacin–Luteolin Lipid Nanoplatform for Photodynamic Therapy of Prostatic CancerInternational Journal of Molecular SciencesAugust 25, 2026Riyad F. Alzhrani, Khalid Alamer, Nasser Alothaymin, Rabbani Syed, Ahmed H. Bakheit, Gamaleldin I. HarisaAssay MMP was evaluated using MMP Assay Kit (MedChemExpress, Monmouth Junction, NJ, USA; Cat. No. HY-K0601) according to the manufacturer’s instructions. The assay is based on the membrane-permeable fluorescent

(7) HAMA microneedles patch loaded with muscle-targeting engineered apoptotic vesicles and attenuating sarcopenia by regulating mitochondrial homeostasisJournal of NanobiotechnologyJuly 24, 2026Jiayao Zhang, Zhao Lin, Haiqi Ding, Mingjuan Zhang, Yibin Su, Linhai Yang, Dingqiang Chen, Xuanhao Chen, Jun Luo, Fuli Wen, et al.mitochondrial membrane potential was assessed using the Mitochondrial Membrane Potential Assay Kit (JC-1 method) (HY-K0601, MCE, USA). Briefly, cells from different treatment groups were incubated with JC-1 staining working

(8) Pyrroloquinoline Quinone Improves Cognitive-Related Behavioral Performance Associated with Enhanced Mitochondrial Bioenergetics in Naturally Aged MiceAntioxidants (Basel)July 24, 2026Yun Yan, Di Deng, Chunxia Tan, Qi Lu, Jiutang Sun, Shaoliang Wu, Zhanhua Jiang, Yibo Li, Tao Luevaluate the mitochondrial membrane potential (ΔΨm), cells were stained with the JC-1 fluorescent probe (HY-K0601; MedChemExpress, Monmouth Junction, NJ, USA) according to the provided guidelines. Fluorescence levels

(9) GPAM mediates mitochondrial dysfunction and the progression of alcoholic liver disease through lipid remodelingSci AdvJune 26, 2026Zibin Zhan, Xuewen Liu, Zehua Li, Xueyan Qiao, Shuo Li, Yu Gong, Luping Yang, Yi Gao, Xianfeng Xia, Kunhao Bai, et al.transmembrane potential was analyzed using a mitochondrial membrane potential detection kit (JC-1) (HY-K0601, MCE) according to the manufacturer’s instructions. Briefly, cells were washed with ice-cold PBS and

(10) YOD1 drives acute kidney injury by deubiquitinating Bax and promoting apoptosis in tubular epithelial cellsTheranosticsJune 25, 2026Ying Zhao, Shijie Fan, Yu Fang, Qingqing Zhao, Qian Zhou, Zheng Xu, Hong Zhu, Guang LiangJapan). Mitochondrial membrane potential (MMP) determination MMP was measured using the fluorescent probe JC-1 (HY-K0601, MCE, New Jersey, USA). The cells cultured in a 6-well plate were incubated with JC-1 (2 μM) at 37 °C

(11) Charge-Competition AIEgens Induce Mitochondrial Dysfunction for Selective Eradication of Candida albicans while Restoring Vaginal MicrobiotaJ Microbiol BiotechnolJune 12, 2026Runjie Zhang, Xiaoxue Li, Xinyi Chen, Aijun Shen, Xuemei Zhang, Chen Peng, Jin QiuMitochondrial Membrane Potential Detection Mitochondrial membrane potential was measured by JC-1 staining (MCE, HY-K0601). The Lactobacillus and C. albicans treated with 100 μM TPE-ET were suspended in 500 μL PBS supplemented

(12) Dual Metabolic Inhibition by Berberine and Glutor Triggers AMPK/JNK-Dependent DNA Damage in Cancer CellsInt J Med SciMay 29, 2026Meina Shi, Chengming Wei, Dayuan Zheng, Dongfan Yang, Tong Chu, Shaokui Liang, Lu Yang, Kuanyun Zhang, Yanchao Yang, Wenzhe MaPotential Assay Mitochondrial membrane potential (ΔΨm) was determined utilizing the JC-1 dye (MCE, HY-K0601). Post-treatment for 24 h, cells were incubated with JC-1 in the dark. Red fluorescence (Ex/Em 560/595

(13) Platelet-derived vesicles restore fertility in endometrial injury by modulating the endometrial immune niche in miceSci AdvMay 29, 2026Dongxiao Li, Yanhong Yang, Yilin Zhang, Ling Zhou, Shuting Gu, Chenmeng Zhou, Bo Tian, Bingbing Wu, Fang Xu, Heng Wang, et al.low potential, producing green fluorescence (excitation/emission = 510/527 nm). The JC-1 probe (2 μM, HY-K0601, MCE) was coincubated with cells at 37°C for 20 min, and photographed with a ZEISS confocal microscopy.

(14) PLSCR3 Deficiency Triggers mtDNA-Driven cGAS-STING Activation to Potentiate Antitumor Immunity in Colorectal CancerHum MutatMay 25, 2026Limian Ling, Jingyu Wu, Lei Bao, Zhaohui Liu, Qun Deng, Xiaowen Ji, Shaojun Yu, Feng Yu, Akao Zhu, Qian Xiao, et al.Mitochondrial Membrane Potential Assay Cells were incubated with JC‐1 dye (MedChemExpress, Cat. No. HY‐K0601) at 37°C in the dark, enabling accumulation in mitochondria; high membrane potential promoted J‐aggregate

(15) NK cell vesicles-based nanoplatform loaded with sorafenib synergizing ferroptosis and antitumor immunity against hepatocellular carcinomaJournal of NanobiotechnologyMay 21, 2026Chulan Gong, Shenjie Zhang, Yuting Fan, Dan Liang, Zailing Yang, Jing Zhang, Xiong Liu, Ting Guo, Xinlan Li, Tao Shen, et al.maintenance medium, NK-NVs, SRB, or NK-NVs-SRB for 48 h. After treatment, JC-1 staining solution (MCE, HY-K0601) was added, and the cells were incubated at 37 °C for 20 min. Mitochondrial membrane potential changes

(16) Pyrroloquinoline Quinone Mitigates Type 2 Diabetes-Induced Cardiac Injury Through Mitochondrial Quality Control and Inhibition of NLRP3-Dependent PyroptosisMetabolitesMay 19, 2026Xue Zhang, Wei Liu, Zhijing Fu, Zhuoling Chen, Qixin Chen, Yanan Shen, Yukai Jin, Dengfeng Xu, Yin Wang, Xuefeng Qu, et al.under HG, PA, and PQQ treatments was assessed using a JC-1 mitochondrial membrane potential assay kit (HY-K0601, MedChemExpress, Shanghai, China). AC16 cells were seeded in six-well culture plates at a density of

(17) GATM alleviates sepsis-induced acute kidney injury via PDK4-mediated glycolytic reprogramming in renal tubular epithelial cellsCellular and Molecular Life SciencesMay 9, 2026Ruhao Yang, Niandan Hu, Hairui Chen, Wenqiang Li, Ting ZhengTUNEL-positive cells per field area. Mitochondrial membrane potential assay (JC-1 assay) JC-1 dye (HY-K0601, MCE) was brought to room temperature and 10 µL of JC-1 (200 µM) was added to each well to achieve a

(18) Mitochondrial Function Under Combined Oral Contraceptive Exposure in a Neuroblastoma Model: A Preliminary InvestigationBiomedicinesMay 7, 2026Francesco Chiara, Sarah Allegra, Francesco Maximillian Anthony Shelton Agar, Giuliana Abbadessa, Silvia De Franciaexperimental conditions were balanced for the final DMSO concentration (≤0.1% v/v). The probes JC-1 (HY-K0601-100T), H2DCFDA (HY-153006), DAF-FM DA (HY-D0717), and Hoechst 33342 (HY-15559) were also purchased from

(19) CD73(high) fibroblasts orchestrate keratinocyte inflammation in the psoriasis-associated epithelial immune microenvironmentNature communicationsApril 9, 2026Tian Y, Guo J, Sun J, Zhang X, Zhou G, Ye L, Zhang Y, Liu P, Zhou J, Xiao C, et al.methyl ester (TMRM) (HY-D0984A; MedChemExpress) and JC-1 Mitochondrial Membrane Potential Assay Kit (HY-K0601; MedChemExpress). In brief, for TMRM staining, cells were trypsinzed after treatment and the pellet

(20) Sunitinib and Fenofibrate as Combination Therapy for MDR Glioblastoma: Insights from In Vitro and In Silico StudiesOncology ResearchMarch 23, 2026Saad Alobid, Hussam Albassam, Tebyan O. Mirgany, Faris Almutairi, Mohammed Mufadhe Alanazi, Ahmed H. Bakheit, Hanadi H. Asiri, Eram Eltahir, Gamaleldin I. Harisastabilization. The MMP Assay Kit purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a Cat. No.: HY-K0601 (JC-1 dye) was employed to evaluate the impact of various treatments on ∆Ψm. JC-1 is a membrane-permeant

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JC-1 Mitochondrial Membrane Potential Assay Kit from MedChemExpress

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JC-1 Mitochondrial Membrane Potential Assay Kit from MedChemExpress

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