JC-1 Mitochondrial Membrane Potential Assay Kit HY-K0601 from MedChemExpress

Product Specs
ItemJC-1 Mitochondrial Membrane Potential Assay Kit
CompanyMedChemExpress
Price $95.00Supplier Page View Company Product Page
Catalog NumberHY-K0601
Quantity100 T
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Galactokinase 1 Inhibition-Induced Cell Cycle Arrest and Apoptosis in Bladder Cancer Cells Is Associated with AKT Signaling DownregulationInt J Mol SciMarch 23, 2026Surya P. Singh, Ronghao Liu, Feng Yan, Qinggong Tang, Chinthalapally V. Rao, Venkateshwar Madka(10 µL) according to the JC-1 Mitochondrial Membrane Potential Assay Kit (MedChemExpress NJ, USA # HY-K0601) and incubated in a CO2 incubator for 20 min. After incubation, the cells were centrifuged for 5 min

(2) Sunitinib and Fenofibrate as Combination Therapy for MDR Glioblastoma: Insights from In Vitro and In Silico StudiesOncology ResearchMarch 23, 2026Saad Alobid, Hussam Albassam, Tebyan O. Mirgany, Faris Almutairi, Mohammed Mufadhe Alanazi, Ahmed H. Bakheit, Hanadi H. Asiri, Eram Eltahir, Gamaleldin I. Harisastabilization. The MMP Assay Kit purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a Cat. No.: HY-K0601 (JC-1 dye) was employed to evaluate the impact of various treatments on ∆Ψm. JC-1 is a membrane-permeant

(3) High-intensity interval training alleviates COPD-induced gastrocnemius muscle dysfunction via the BRD4/PGC-1α axis through restoring mitochondrial function and oxidative fiber compositionJ Muscle Res Cell MotilMarch 21, 2026Chunjiao Zhu, Wenna Peng, Liting Yang, Wanwan Zhangthis study, Changes in MMP were determined using a JC-1 Mitochondrial Membrane Potential Assay Kit (HY-K0601, MCE, China) according to the manufacturer’s instructions. For cells, after JC-1 staining, cells with

(4) Novel Adenine-Hydrazone Hybrids Against Human Lung Adenocarcinoma (A549): Design, Synthesis, Cellular Mechanistic Investigation and Molecular Docking StudiesPharmaceuticals (Basel)March 13, 2026Emre Menteşe, Nedime Çalışkan, Didem Aksu, Mustafa Emirik, Adem Güner, Fatih YılmazPotential (MMP, ΔΨm) Mitochondrial membrane potential (ΔΨm) was evaluated using the JC-1 dye (Cat. No. HY-K0601, MedChemExpress, Monmouth Junction, NJ, USA) according to the manufacturer’s instructions. Cells were

(5) VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagyCancer Biology & MedicineFebruary 17, 2026Yanna Bi, Sisi Wei, Zhe Zhang, Yuqing Wang, Yang Wen, Hongquan Wang, Kexin Li, Cong Zhang, Yumin Wang, Lianmei Zhao, et al.potential (Δψm) Take an aliquot of cell suspension and add an equal volume of JC-1 working solution (5 μM, HY-K0601; MCE), mixing gently. In a separate cell sample, pretreat with CCCP (final concentration typically 10–50

(6) Liposome-Encapsulated Rutin Attenuates Cisplatin-Induced Ototoxicity via Suppression of P53-Associated Oxidative InjuryBiomaterials ResearchFebruary 10, 2026Bo Liu, Yaqin Tu, Xiangrui Li, Wenting Yu, Wenqing Zou, Wei Tang, Shimin Zong, Songwei Tan, Hongjun Xiaomitochondrial membrane potential (MMP) was assessed with a JC-1 fluorescent probe (MedChemExpress, China, HY-K0601). OC-1 cells were cultured in 24-well plates and treated with either 10 μM CDDP or 50 μM Lip-Rutin prior

(7) Functional analysis and identification of miRNAs associated with lipid metabolism from milk-derived exosomesJ Anim Sci BiotechnolFebruary 4, 2026Xin Lu, Tianyu Deng, Yue Liu, Xiaohan Zhang, Xue Bai, Xibi Fang, Runjun Yangpotential was assessed 48 h post-transfection using the JC-1 mitochondrial membrane potential assay kit (HY-K0601, MedChemExpress) according to the manufacturer's instructions. The pre-aliquoted JC-1 solution (200

(8) Fully human anti-B7-H4 antibody induces lysosome-dependent ferroptosis to reverse primary resistance to PD-1 blockadeJournal for ImmunoTherapy of CancerJanuary 12, 2026Renlu Zhang, Yang Wang, Yue Wu, Bingyu Zheng, Qiumei Cao, Yunhan Chen, Zhengyun Cao, Yanyang Zhu, Linlin Zhou, Qiuyu ZhangB7-H4 antibodies for 2 hours and then stained using JC-1 Mitochondrial Membrane Potential Assay Kit (#HY-K0601, MedChemExpress). Fluorescence was measured by flow cytometry (excitation: 510 nm, emission: 585 nm). Glutamate

(9) Mechanistic study of glutamine metabolic reprogramming driving non-small cell lung cancer progression via the FGF17-FGFR4 axis mediating epithelial-mesenchymal transitionFrontiers in Molecular BiosciencesJanuary 2, 2026Qinghua Kong, Xiaoyan Wang, Wei Ding(HY-D0940, MCE), GSH/GSSG Assay Kit (HY-K0311, MCE), JC-1 mitochondrial membrane potential assay kit (HY-K0601, MCE), were employed to assess metabolic and redox parameters in NCI-H1299 cells or mice tutors tissue

(10) Puerarin Targets MIC19 to Suppress Mitochondrial Metabolism of Tumor‐Infiltrating Tregs and Enhance Anti‐tumor ImmunityAdvanced ScienceJanuary 1, 2026Yu Li, Ziyan Song, Jianjun Ding, Yiheng Zhou, Tianning Huang, Qufei Qian, Miao Yu, Wenzhao Chen, Jiazheng Liu, Ling Lu, et al.Mitochondrial membrane potential was assessed using the JC‐1 Mitochondrial Membrane Potential Assay Kit (MCE, HY‐K0601) according to the manufacturer's instructions. Fluorescent images were acquired and analyzed using ImageJ

(11) Small Extracellular Vesicles From Human Amniotic Membrane Mesenchymal Stem Cells Rejuvenate Senescent β Cells and Cure Age-Related Diabetes in MiceAging CellDecember 14, 2025Lei Xiao, Zicheng Zhang, Tong Li, Yuyin Jiang, Yuanxin Liu, Tingting Lv, Lianju Qin, Yunxia Zhu, Wei TangAssessment Mitochondrial membrane potential (ΔΨm) in treated MIN6 cells was assessed using the JC‐1 assay kit (Cat#HY‐K0601, MedChemExpress, USA) per manufacturer's protocol, with 2 μM JC‐1 staining (37°C, 15–20 min) followed

(12) MYC affects mitochondrial function in IgA nephropathy by promoting the degradation of MFN1 through HRD1Immunologic ResearchDecember 1, 2025Xueping Wu, Lei Liu, Ruiping Zhao, Weidong Chenintensity of MitoSOX. JC-1 staining The transfected HK2 cells were incubated with JC-1 solution (MCE, HY-K0601) for 30 min at 37℃, and incubated with Hoechst solution for 10 min. The samples were observed under

(13) MiR-24 Attenuates Oxidative Stress and Mitochondrial Apoptosis in Ventilator-Induced Lung Injury by Targeting Bcl-2-related Ovarian KillerPulm CircOctober 7, 2025Wenbo Xu, Wenjiao Ren, Lingling Zhang, Bing Wang, Linqi Gao, Dong YuanUSA). 2.13 Mitochondrial Membrane Potential (MMP) Assay The MMP was evaluated using JC‐1 staining (Cat# HY‐K0601, MCE, USA). Cells were incubated with JC‐1 working solution for 30 min. Following incubation, cells

(14) Branched-Chain α Keto-Acid Dehydrogenase Kinase-Mediated AKT Phosphorylation Promotes RCC Tumorigenesis and Drug ResistanceAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2025Tian Q, Wang J, Li Q, Liu Y, Chen Y, Feng J, Lei ZN, Patel H, Cai CY, Xu Y, et al.Potential The cell apoptosis was detected using the JC‐1 Mitochondrial Membrane Potential Assay Kit (MCE, HY‐K0601) following the manufacturer's instructions. Cells were plated in 6‐well plates and cultured at 37 °C

(15) Self-assembled cyanidin-3-O-glucoside nanoparticles alleviate inflammation and ferroptosis induced by PRRSV infectionJournal of VirologySeptember 23, 2025Xiaohan Chen, Yipeng Pang, Fructueux Modeste Amona, Zilu Liu, Fang Wang, Yuan Liang, Jiachen Yang, Wanhan Zhang, Xingtang Fang, Xi Chenmitochondrial membrane potential (ΔΨm) was assessed using the lipophilic fluorescent dye JC-1 (MedChemExpress, HY-K0601) as previously described (33). Marc-145 cells were seeded in a 24-well plate at a 5 × 104 cells/well

(16) EZH1 deficiency promotes ferroptosis resistance by activating NRF2 in sepsis-associated liver injuryClin EpigeneticsJune 9, 2025Meihua Mei, Ying You, Ningxin Tan, Xiaoshun He, Junqi Huangmitochondrial membrane potential probe Cultivate 7.5 × 105 cells in each 12 well plate. Dissolve JC-1 powder (HY-K0601, MCE) in DMSO to prepare a 200 μm stock solution. Then, use complete culture medium to dilute 200 μm

(17) Melatonin Improves Semen Quality by Modulating Oxidative Stress, Endocrine Hormones, and Tryptophan Metabolism of Hu Rams Under Summer Heat Stress and the Non-Reproductive SeasonAntioxidants (Basel)May 24, 2025Qian-Qiu Liu, Xiong Li, Jia-Hao Li, Yang Zhou, Ming-Kai Lei, Wei-Qi Yin, You-She Ren, Chun-He Yang, Chun-Xiang Zhangrecorded on an optical microscope. Mitochondrial membrane integrity (MI) was measured by the JC/PI (HY-K0601 and HY-D0815, MedChemExpress Asia Agent, Shanghai, China) staining method [36]. DNA integrity (DNAI)

(18) Evernic Acid: A Low-Toxic and Selective Alternative to Chemotherapeutic Agents in the Treatment of Ovarian CancerArch Pharm (Weinheim)May 22, 2025Mine Ensoy, Damla Nur Parıltı, Ayşe Hale Alkan, Kübra Nur Kaplan İlhan, Pelin Mutlu, Bala Gür Dedeoğlu, Demet Cansaran-DumanAssay Early apoptotic cells were assessed using an MMP assay kit containing JC‐1 (MedChemExpress cat no: HY‐K0601‐100T), a fluorescent probe for rapid and sensitive detection of changes in mitochondrial membrane potential

(19) Response to Oxidative Stress Induced by Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in Differentiated PC12 CellsToxicsApril 27, 2025Cunzhi Li, Xiaoqiang Lv, Zhiyong Liu, Hui Deng, Ting Gao, Huan Li, Xinying Peng, Airong Qian, Junhong Gao, Lifang HuShanghai, China). JC-1 Mitochondrial Membrane Potential Assay Kit was provided by MedChemExpress (#HY-K0601, MCE, Shanghai, China). ROS (#ML926281), MDA (#ML077384-2), GSH (#ML531010-2) and SOD (#ML077379-2)

(20) Redox disruption using electroactive liposome coated gold nanoparticles for cancer therapyNat CommunApril 5, 2025Ying-Chi Chen, Li-Chan Chang, Yan-Ling Liu, Ming-Che Chang, Yin-Fen Liu, Po-Ya Chang, Divinah Manoharan, Wen-Jyun Wang, Jia-Sin Chen, Hsueh-Chun Wang, et al.post-treatment, the cancer cells were stained with a JC-1 mitochondrial membrane potential assay kit (HY-K0601, MedChemExpress) according to the manufacturer’s guidelines. The images were observed using an inverted

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JC-1 Mitochondrial Membrane Potential Assay Kit from MedChemExpress

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JC-1 Mitochondrial Membrane Potential Assay Kit from MedChemExpress

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