Fig 1: IL-10 mediates GC loss driven by NC NF-κB hyperactivation in B cells(A) FC analysis of intracellular IL-10 expression in activated B cells. Geometric mean fluorescence intensity (gMFI).(B) ELISA results for IL-10 in the conditioned supernatant from ex vivo cultured FOB.(C) FC analysis of regulatory B cells relative abundance.(D) RNAseq analysis of Il10 mRNA expression levels in the indicated model.33(E) RNAseq analysis of IL10 mRNA expression levels in the indicated model (Li et al., 2025). Fragments per kilobase million (FPKM).(F) ChIPseq analysis of RelB binding at the IL10 locus.34 An ATACseq track is included to indicate accessible regions in the same model. Representative tracks are shown. Black boxes indicate significant peaks called by MACS2.35(G) FC analysis of intracellular IL-10 expression in TFR cells.(H) Experimental scheme for I-K.(I and K) FC analysis of (I) TFR cells, (J) TFH cells, or (K) GCB, in animals treated as indicated in (H).Individual dots represent biological replicates. Values represent mean ± SEM. Data are reproducible with two repeats. NS, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001, using Mann-Whitney U-test (A), unpaired two-tailed Student’s t test (B, C, G), one-way ANOVA with Tukey’s post-test (D, E), or two-way ANOVA with Tukey’s post-test (I-K).