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LunaScript RT SuperMix
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LunaScript RT SuperMix
LunaScript RT SuperMix from New England Biolabs
Be the first to write a review!
Citations:
(41)
$403.00 Inquire
from
New England Biolabs for
LunaScript RT SuperMix
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
LunaScript RT SuperMix
Company
New England Biolabs
Price
$403.00 Inquire
Catalog Number
M3010L
Temperature
-20 C
Applications
DNA Analysis, RT-PCR
Quantity
100 reactions
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (41)
(1)
Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma
J Immunother Cancer
March 27, 2026
Mark Gregory, Justin B Hackett, Savannah Hughes, Maria C Muñiz, Samantha Ayoub, Alexander Boerner, Habib Alrabaa, Lucien Taylor, Brianna Rivera, Hanna Holland,
et al.
Complementary DNA (cDNA) was prepared using
LunaScript RT SuperMix
Kit (
New England Biolabs
, cat. #
M3010L
). Quantitative reverse transcription
PCR
was performed using
iTaq Universal Probes Supermix
(
Bio-Rad
,
(2)
Nationwide wastewater sequencing surveillance of SARS-CoV-2 lineages: validation against clinical data across 28 U.S. states
PeerJ
March 13, 2026
Alessandro Zulli, Bradley White, Dorothea Duong, Bridgette Shelden, Vikram Chan-Herur, Marlene Wolfe, Alexandria B. Boehm
beads (Roche, Basel, Switzerland). Reverse transcription was done using
LunaScript RT SuperMix
(
NEB
M3010
) in a 13.75 µL reaction. cDNA was purified from the reverse transcription reaction using 2X HyperPure
(3)
Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior
Journal of Functional Biomaterials
February 25, 2026
Leila Mohammadnejad, Madeline Mangold, Hannah Conrady, Wafa Zafira, Evi Kimmerle-Mueller, Peter Schneider, Barbara Illing, Christiane von von Ohle, Annika Hechler, Frank Rupp,
et al.
using 350 ng of RNA with the
LunaScript RT SuperMix
(
New England Biolabs
, Ipswich, MA, USA; Cat. No.
M3010X
).
qRT-PCR
was carried out using the
Luna Universal qPCR Master Mix
(
New England Biolabs
, Ipswich, MA,
(4)
Antiviral Role of Surface Layer Protein A (SlpA) of Lactobacillus acidophilus
Pathogens
January 19, 2026
Govindaraj Anumanthan, Ananta Prasad Arukha, Ayalew Mergia, Bikash Sahay
converted into cDNA for
quantitative evaluation
of transcripts using
LunaScript RT SuperMix
(Cat #
M3010L
;
NEB
biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. The concentration of isolated
(5)
Photobiomodulation-Driven Tenogenic Differentiation of MSCs in Hydrogel Culture
Int J Mol Sci
December 12, 2025
Brendon Roets, Heidi Abrahamse, Anine Crous
microgram of total RNA using the
LunaScript® RT SuperMix
Kit (
New England Biolabs
, Ipswich, MA, USA,
NEB
M3010X
), on a Stratagene thermocycler (Agilent Technologies, Santa Clara, CA, USA, Mx3005P).
RT-qPCR
was performed
(6)
STING induces ZBP1-mediated necroptosis independently of TNFR1 and FADD
Nature
November 20, 2025
Konstantinos Kelepouras, Julia Saggau, Debora Bonasera, Christine Kiefer, Federica Locci, Hassan Rakhsh-Khorshid, Louisa Grauvogel, Ana Beatriz Varanda, Martin Peifer, Elena Loricchio,
et al.
according to manufacturer’s instructions.
RT–qPCR
cDNA was prepared using the
Lunascript RT SuperMix
kit (
M3010
,
New England Biolabs
).
RT–qPCR
was performed using
Luna Universal qPCR Master Mix
(M3003,
New England Biolabs
(7)
Molecular Epidemiology of SARS-CoV-2 in Northern Greece from the Index Case up to Early 2025 Using Nanopore Sequencing
Epidemiologia (Basel)
November 12, 2025
Georgios Meletis, Styliani Pappa, Georgia Gioula, Maria Exindari, Maria Christoforidi, Anna Papa
undiluted.
cDNA synthesis
was performed using 16 μL of viral RNA mixed with 4 μL of
LunaScript RT SuperMix
(
M3010
,
NEB
, Ipswich, MA, USA), following the manufacturers guidelines. Following
cDNA synthesis
, the overlapping
(8)
Differential Control of Growth and Identity by HNF4α Isoforms in Pancreatic Ductal Adenocarcinoma
Molecular Cancer Research
November 3, 2025
Pengshu Fang, Emily R. Wilson, Sydney N. Larsen, Walter A. Orellana, Margaret A. Hall, Chris Stubben, Acramul Haque Kabir, Kajsa Affolter, Richard A. Moffitt, Xiaoyang Zhang,
et al.
manufacturer’s specifications. cDNA was synthesized from Trizol-extracted RNA using
LunaScript RT SuperMix
(
NEB
,
M3010
) according to the manufacturer’s specifications.
qPCR
was performed on cDNA using
Luna Universal qPCR Master Mix
(9)
RNA G‐Quadruplex RIBOTAC‐Mediated Targeted Degradation of lncRNA TERRA
Advanced Science
October 6, 2025
Elias Khaskia, Dipak Dahatonde, Raphael I. Benhamou
was performed on 1 µg of total RNA using the
LunaScript RT SuperMix
Kit (
New England BioLabs
, cat #
M3010
), following the manufacturer's instructions. Approximately 30 ng of cDNA was used for each
qPCR
reaction,
(10)
Opposing lineage specifiers induce a protumor hybrid identity state in lung adenocarcinoma
Genes & Development
September 2, 2025
Gabriela Fort, Henry Arnold, Soledad A. Camolotto, Kayla O'Toole, Rushmeen Tariq, Anna Waters, Katherine Gillis, Eric L. Snyder
manufacturer's specifications. cDNA was synthesized from TRIzol-extracted RNA using
LunaScript RT SuperMix
(
NEB
M3010
) according to the manufacturer's specifications.
qPCR
was performed on cDNA using
Luna Universal Probe qPCR master mix
(11)
Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cells
Nature communications
August 27, 2025
Kowar A, Becker JP, Del Pizzo R, Tang Z, Champagne J, Wellach K, Samimi K, Galindo-Albarrán A, Körner PR, Montenegro Navarro J,
et al
.
Quantitative real-time PCR
A total of 500 ng of RNA was reverse-transcribed using
LunaScript RT Supermix
(
NEB
,
M3010L
).
Quantitative real-time PCR
was subsequently performed with Luna Universal
qPCR
Mix (
NEB
,
M3003X
).
(12)
Evaluating T1/T2 Relaxometry with OCRA Tabletop MRI System in Fresh Clinical Samples: Preliminary Insights into ZEB1-Associated Tissue Characteristics
Technol Cancer Res Treat
August 26, 2025
Ahmed Y. Sanin, Marcus Prier, Thomas Wartmann, Christian Siba, Katrin Hippe, Maciej Pech, Roland S. Croner, Oliver Speck, Ulf D. Kahlert, Georg Rose
adhering to manufacturer's instructions. RNA was reverse-transcribed using
LunaScript® RT SuperMix
(
NEB
, #
M3010L
). ZEB1 mRNA expression was quantified via
Luna Universal qPCR Master Mix
(
NEB
) with primers (Microsynth
(13)
ZEB1 stratifies the response to Sorafenib and Mdivi-1 combination therapy in hepatocellular carcinoma
Sci Rep
August 19, 2025
H. Freudenstein, M. Strecker, S. Gylstorff, W. Shi, M. Boettcher, S. Medunjanin, C. Catapano, C. Siba, C. Wex, T. Wartmann,
et al.
transcription was performed using 1 µg of RNA and the
New England BioLabs
LunaScript® RT Super Mix (cat. No.
M3010
) according to manufacturer’s instruction. Primers used in this study can be found in Supplementary Table
(14)
Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes
Current Biology
August 4, 2025
Philip S. Goff, Shyamal Patel, Dawn C. Harper, Tom Carter, Michael S. Marks, Elena V. Sviderskaya
assays
RNeasy Plus Mini Kit,
Qiagen
,Cat. #
74134
LunaScript RT SuperMix
Kit,
New England Biolabs
,Cat. #
M3010
Luna Universal qPCR Master Mix
,
New England Biolabs
,Cat. # M3003 ,,
Experimental models
: Cell lines,
Experimental models
(15)
Reconciling competing models on the roles of condensates and soluble complexes in transcription factor function
Molecular Cell
July 17, 2025
Anne Bremer, Walter H. Lang, Ryan P. Kempen, Kumari Sweta, Aaron B. Taylor, Madeleine B. Borgia, Aseem Z. Ansari, Tanja Mittag
Mix,
NEB
,Cat#
E2621
BP Clonase™ II Enzyme Mix,
Invitrogen
,Cat#
11789020
Luna Script® RT Super Mix 5x,
NEB
,Cat#
M3010
2x
Luna® Universal qPCR Master Mix
,
NEB
,Cat# M3003
Dynabeads™ Antibody Coupling Kit
,
Invitrogen
,Cat#
14311D
Deposited
(16)
Genomic and Single-Cell Analyses Characterize Patient-Derived Tumor Organoids to Enable Personalized Therapy for Head and Neck Squamous Cell Carcinoma
Cancer Research
July 15, 2025
Jung Hyun Um, Yueyuan Zheng, Qiong Mao, Chehyun Nam, Hua Zhao, Yoon Woo Koh, Su-Jin Shin, Young Min Park, De-Chen Lin
Cat#70106).
cDNA synthesis
was performed using the Luna script RT Supermix kit (
New England Biolabs
, Cat#
M3010L
).
Quantitative real-time PCR
(
qRT-PCR
) was performed by using the
Powerup™ SYBR Green Master Mix
(
Thermo Fisher Scientific
(17)
Incidence of Human Sapovirus Outbreaks in Beijing, China, 2014–2021: Predominance of Genotypes GII.3 and GI.2
Emerging Microbes & Infections
June 23, 2025
Lingyu Shen, Fan Yu, Hanqiu Yan, Baiwei Liu, Weihong Li, Yu Wang, Yi Tian, Lei Jia, Peng Yang, Quanyi Wang,
et al.
respectively. Reverse transcription was performed using the
LunaScript® RT SuperMix
Kit (Cat. No.
NEB
#
M3010
;
New England Biolabs
, Massachusetts, USA) in a 10 μL reaction volume, with odd-numbered primers used
(18)
Pancreatic cancer cachexia is mediated by PTHrP-driven disruption of adipose de novo lipogenesis
bioRxiv
June 7, 2025
Nikita Bhalerao, Yamini Ogoti, Jessica Peura, Calvin Johnson, Qingbo Chen, Ekaterina D. Korobkina, Faith N. Keller, Maximilian Wengyn, Robert J. Norgard, Claire Shamber,
et al.
cDNA was prepared using 100ng-1000ng RNA using the
New England Biolabs
LunaScript RT supermix
(Cat#
M3010L
).
Quantitative PCR
was setup using
Luna Universal qPCR Master Mix
(Cat #
M3003E
). The following primers
(19)
Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironment
Nature communications
May 19, 2025
Aviles-Huerta D, Del Pizzo R, Kowar A, Baig AH, Palazzo G, Stepanova E, Amaya Ramirez CC, D'Agostino S, Ratto E, Pechincha C,
et al
.
(Thermo Fisher). 600 ng of total RNA was reversed transcribed using
LunaScript RT Supermix
(
NEB
; cat.
M3010L
) and
quantitative real-time PCR
was performed using Luna Universal
qPCR
Mix (
NEB
; cat.
M3003X
). Ct values
(20)
Neonatal gut microbiota succession in mice mapped by maturation, site, injury, and single immunoglobulin interleukin-1 related receptor genotype
iScience
April 18, 2025
Shahid Umar, Wei Yu, Hao Xuan, Ishfaq Ahmed, Cuncong Zhong, Michael Morowitz, Mathew Brian Rogers, Mark Ivan Attard, Venkatesh Sampath
proteins" ,, Esbilac canine milk replacer,PetAg,N/A TRiZol,
ThermoFisher
,
15596018
cDNA reverse transcription kit,
NEB
,
M3010
FLUORO-GEL,
Electron Microscopy
Science,1798510 ,, Critical commercial
assays
,Critical commercial
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LunaScript RT SuperMix from New England Biolabs
Price
$403.00
Inquire
Product Specs
Company Info
Citations
Reviews
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