LunaScript RT SuperMix M3010L from New England Biolabs

Product Specs
ItemLunaScript RT SuperMix
CompanyNew England Biolabs
Price $403.00 Inquire
Catalog NumberM3010L
Temperature-20 C
ApplicationsDNA Analysis, RT-PCR
Quantity100 reactions

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) IFNα and bleomycin-induced DNA damage activate distinct stress responses in human islets and induced pluripotent stem cell-derived islet cellsIsletsDecember 31, 2026Roozbeh Akbari Motlagh, Nayara Rampazzo Morelli, Nicole Alcasid, Peter J. ThompsonNanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific). LunaScript® RT SuperMix (New England Biolabs Cat# M3010) was used for cDNA synthesis from approximately 100–200 ng of total RNA using the same amount of total

(2) Evaluating exon skipping in the central nervous system in Duchenne muscular dystrophy using spatial transcriptomicsiScienceAugust 21, 2026Qirong Mao, Alireza Ahmadi, Sharon de Vries, Laura G.M. Heezen, Ophélie Vacca, Mathilde Doisy, Annemieke Aartsma-Rus, Maaike van Putten, Aurélie Goyenvalle, Ahmed Mahfouz, et al.Panel (up to 50 genes),10x Genomics,Cat# 1000464 LunaScript® RT SuperMix Kit,New England Biolabs,"Cat# M3010L, M3010X, M3010E" GoTaq® G2 Colorless Master Mix,Promega,Cat# M7833 ddPCR Supermix for Probes (No dUTP),Bio-Rad,Cat#

(3) Connexin 50 mediates disease-relevant alpha-synuclein oligomer propagation and neuroinflammation in neurodegenerative diseaseiScienceJuly 17, 2026Nicha Puangmalai, Urmi Sengupta, Nemil Bhatt, Nopparat Suthprasertporn, Fadhl Al-Shaebi, Nikita Shchankin, Jia Yi Liew, Twisha Chatterjee, Dhvani Pansuriya, Ahmet E. Balci, et al.manufacturer’s instructions (Invitrogen). cDNA was synthesized by using Lunascript RT Supermix (NEB M3010) according to manufacturer instructions. The cDNA was amplified using specific primer sets (Table S5)

(4) Subacute Exposure to Aldrin, Endosulfan and Lindane Differentially Alters Metabolic and Inflammatory Gene Expression in White and Brown Adipose Tissue of Male RatsCell Biochem FunctJuly 17, 2026Nada Abdelhamid Mohamed Boujanah, Cihan Suleyman Erdogan, Engin Sumer, Cansu Yakin, Bayram Yilmazusing equal amounts of RNA (200 ng) according to manufacturer's instructions (LunaScript® RT SuperMix, M3010, New England Biolabs). Sequences of transcripts of Pparg, Irisin, Ucp1, Ucp3, Il‐1b, Tnf‐a, Il‐10 and

(5) The heat shock factor Cbr-HSF-1 is necessary for lifespan maintenance in C. briggsaeMicroPubl BiolJune 27, 2026Harvir Bhullar, Jordan Dias, Khushi Pastagia, Wouter van den Berg, Bhagwati P Guptaextraction, cDNA was synthesized using the LunaScript RT SuperMix Kit (New England Biolabs, catalog number: M3010L), and gene expression was quantified by RT-qPCR using the Bio-Rad cycler CFX 96 and the SensiFAST SYBR

(6) Induced chromosomal instability in triple negative breast cancer cells promotes M2-like polarization of macrophagesScientific ReportsJune 6, 2026Ruifang Tian, Siqi Zheng, Floris FoijerComplementary DNA (cDNA) was synthesized from 1.5 µg of RNA using the LunaScript RT SuperMix Kit (NEB, M3010X) in a total reaction volume of 20 µL. qPCR was carried out with iTaq Universal SYBR Green Supermix (Biorad,

(7) Generative modeling for RNA splicing prediction and designElifeMay 29, 2026Di Wu, Natalie Maus, Anupama Jha, Kevin Yang, Benjamin D Wales-McGrath, San Jewell, Anna Tangiyan, Peter Choi, Jake R Gardner, Yoseph BarashRNA Purification Kit (Zymo Research, D2052). RNA was converted to cDNA with the LunaScript RT SuperMix (NEB, M3010L), and PCR for the splicing event was performed with the Q5 Hot Start High-Fidelity 2 X Master Mix (NEB,

(8) Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choiceNature MetabolismApril 29, 2026Milan R. Savani, Bingbing Li, Bailey C. Smith, Wen Gu, Yi Xiao, Gerard Baquer, Tracey Shipman, Skyler S. Oken, Namya Manoj, Lauren G. Zacharias, et al.#1 cells with the RNeasy Mini kit (QIAGEN, 74004) and LunaScript RT Supermix (New England Biolabs, M3010) was used to provide template. The following primers were designed to append 5’ attB1 and 3’ HA tag

(9) Assessing frailty in aged zebrafish using a quick pseudo-frailty indexBiology OpenApril 15, 2026Anne Cathrine Hyde, Pamela Ellis, Sherif El-Khamisy, Claire Allen, Catarina M. Henriques, Fredericus J. van Eedenwere used as the control group. 470 ng/µl RNA was converted to cDNA using LunarScript RT SuperMix (M3010L, NEB) kit in a 10 µl reaction following the kit instructions. cDNA was stored at −20°C. qPCRs performed

(10) Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesiclesThe EMBO JournalApril 13, 2026Siqi Zheng, Ruifang Tian, Marsudi Siburian, Anna Haider Rubio, Yuanyuan Liu, Rene Wardenaar, Marjan Shirzai, Laura Kempe, Emma Dijkstra, Eliza Warszawik, et al.R0101 Polybrene,Sigma-Aldrich,Cat# 107689 0.45 µm filter,TPP,Y99745 RNeasy Plus kit,Qiage,74136 LunaScript RT SuperMix,NEB,M3010X iTaq Universal SYBR Green Supermix,Bio-Rad,1725124 NEBNext Multiplex Oligos for Illumina,NEB,E6440L Human

(11) Identification and characterization of BRAF⇔TP53 interactions in melanomaCell ReportsApril 1, 2026Kayla T. O’Toole, Adamaris Martinez, Brandon Murphy, Gabriela Fort, Fatima Al-Sudani, Anastasia Prokofyeva, Sanjana Boggaram, Eric A. Smith, Elliott L. Paine, Deevya Baral, et al.(ThermoFisher Scientific). cDNA was synthesized from Trizol-extracted RNA using LunaScript RT SuperMix (NEB, M3010) according to manufacturer’s specifications. qPCR was performed on cDNA using Luna Universal Probe qPCR Master Mix

(12) Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanomaJ Immunother CancerMarch 27, 2026Mark Gregory, Justin B Hackett, Savannah Hughes, Maria C Muñiz, Samantha Ayoub, Alexander Boerner, Habib Alrabaa, Lucien Taylor, Brianna Rivera, Hanna Holland, et al.Complementary DNA (cDNA) was prepared using LunaScript RT SuperMix Kit (New England Biolabs, cat. #M3010L). Quantitative reverse transcription PCR was performed using iTaq Universal Probes Supermix (Bio-Rad,

(13) Nationwide wastewater sequencing surveillance of SARS-CoV-2 lineages: validation against clinical data across 28 U.S. statesPeerJMarch 13, 2026Alessandro Zulli, Bradley White, Dorothea Duong, Bridgette Shelden, Vikram Chan-Herur, Marlene Wolfe, Alexandria B. Boehmbeads (Roche, Basel, Switzerland). Reverse transcription was done using LunaScript RT SuperMix (NEB M3010) in a 13.75 µL reaction. cDNA was purified from the reverse transcription reaction using 2X HyperPure

(14) Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage BehaviorJournal of Functional BiomaterialsFebruary 25, 2026Leila Mohammadnejad, Madeline Mangold, Hannah Conrady, Wafa Zafira, Evi Kimmerle-Mueller, Peter Schneider, Barbara Illing, Christiane von von Ohle, Annika Hechler, Frank Rupp, et al.using 350 ng of RNA with the LunaScript RT SuperMix (New England Biolabs, Ipswich, MA, USA; Cat. No. M3010X). qRT-PCR was carried out using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA,

(15) Antiviral Role of Surface Layer Protein A (SlpA) of Lactobacillus acidophilusPathogensJanuary 19, 2026Govindaraj Anumanthan, Ananta Prasad Arukha, Ayalew Mergia, Bikash Sahayconverted into cDNA for quantitative evaluation of transcripts using LunaScript RT SuperMix (Cat # M3010L; NEB biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions. The concentration of isolated

(16) GPNMB is a biomarker for lysosomal dysfunction and is secreted via LRRK2-modulated lysosomal exocytosisScience AdvancesDecember 19, 2025Erin C. Bogacki, Gustavo Morrone Parfitt, Adriana Cunha, George Longmore, Selina Wray, Patrick A. Lewis, Susanne Herbstpurification kit from NEB (#T2010S) and converted into cDNA using the LunaScript RT SuperMix (NEB, #M3010L) according to the manufacturer’s instructions. Reverse transcription polymerase chain reaction (RT-PCR)

(17) Photobiomodulation-Driven Tenogenic Differentiation of MSCs in Hydrogel CultureInt J Mol SciDecember 12, 2025Brendon Roets, Heidi Abrahamse, Anine Crousmicrogram of total RNA using the LunaScript® RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA, NEB M3010X), on a Stratagene thermocycler (Agilent Technologies, Santa Clara, CA, USA, Mx3005P). RT-qPCR was performed

(18) Molecular Epidemiology of SARS-CoV-2 in Northern Greece from the Index Case up to Early 2025 Using Nanopore SequencingEpidemiologia (Basel)November 12, 2025Georgios Meletis, Styliani Pappa, Georgia Gioula, Maria Exindari, Maria Christoforidi, Anna Papaundiluted. cDNA synthesis was performed using 16 μL of viral RNA mixed with 4 μL of LunaScript RT SuperMix (M3010, NEB, Ipswich, MA, USA), following the manufacturers guidelines. Following cDNA synthesis, the overlapping

(19) Differential control of growth and identity by HNF4α isoforms in pancreatic ductal adenocarcinomaMol Cancer ResNovember 3, 2025Pengshu Fang, Emily R. Wilson, Sydney N. Larsen, Walter A. Orellana, Margaret A. Hall, Chris Stubben, Acramul Haque Kabir, Kajsa Affolter, Richard A. Moffitt, Xiaoyang Zhang, et al.manufacturer’s specifications. cDNA was synthesized from Trizol-extracted RNA using LunaScript RT SuperMix (NEB, M3010) according to the manufacturer’s specifications. qPCR was performed on cDNA using Luna Universal qPCR Master Mix

(20) Differential Control of Growth and Identity by HNF4α Isoforms in Pancreatic Ductal AdenocarcinomaMolecular Cancer ResearchNovember 3, 2025Pengshu Fang, Emily R. Wilson, Sydney N. Larsen, Walter A. Orellana, Margaret A. Hall, Chris Stubben, Acramul Haque Kabir, Kajsa Affolter, Richard A. Moffitt, Xiaoyang Zhang, et al.manufacturer’s specifications. cDNA was synthesized from Trizol-extracted RNA using LunaScript RT SuperMix (NEB, M3010) according to the manufacturer’s specifications. qPCR was performed on cDNA using Luna Universal qPCR Master Mix

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LunaScript RT SuperMix

LunaScript RT SuperMix from New England Biolabs

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LunaScript RT SuperMix from New England Biolabs

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