LunaScript RT SuperMix M3010L from New England Biolabs

Product Specs
ItemLunaScript RT SuperMix
CompanyNew England Biolabs
Price $403.00 Inquire
Catalog NumberM3010L
Temperature-20 C
ApplicationsDNA Analysis, RT-PCR
Quantity100 reactions

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Cleavage and polyadenylation factors are potential regulators of adipogenesisBMC Research NotesSeptember 2, 2024Salwa Mohd Mostafa, Claire MooreRNA was then reverse transcribed to cDNA using NEB LunaScript® RT SuperMix kit (New England Biolabs, M3010L), and qPCR was carried out using NEB Luna® Universal qPCR Master Mix (New England Biolabs, M3003L) and

(42) Reconstructing SARS-CoV-2 lineages from mixed wastewater sequencing dataScientific ReportsAugust 31, 2024Isaac Ellmen, Alyssa K. Overton, Jennifer J. Knapp, Delaney Nash, Hannifer Ho, Yemurayi Hungwe, Samran Prasla, Jozef I. Nissimov, Trevor C. Charles\begin{document}$$\circledR$$\end{document}® RT SuperMix Kit, according to the manufacturer’s protocol (New England Biolabs, M3010L). SARS-CoV-2 cDNA was then amplified by PCR using the ARTIC V4.1 primers (IDT, 10011442) and Q5 High Fidelity 2X Master Mix

(43) Suppression of inner blood-retinal barrier breakdown and pathogenic Müller glia activation in ischemia retinopathy by myeloid cell depletionJournal of NeuroinflammationAugust 24, 2024Lingli Zhou, Zhenhua Xu, Haining Lu, Hongkwan Cho, Yangyiran Xie, Grace Lee, Kaoru Ri, Elia J. Duhextraction with RNeasy Micro kit (QIAGEN). cDNA was synthesized with LunaScript® RT SuperMix Kit (NEB #M3010). Real-time PCR analysis for retina tissue RNeasy mini kit (QIAGEN) was used for RNA isolation from

(44) Inferring enterovirus D68 transmission dynamics from the genomic data of two 2022 North American outbreaksnpj VirusesAugust 2, 2024Martin Grunnill, Alireza Eshaghi, Lambodhar Damodaran, Sandeep Nagra, Ali Gharouni, Thomas Braukmann, Shawn Clark, Adriana Peci, Sandra Isabel, Philip Banh, et al.St-Laurent, Quebec, Canada) and reverse transcribed into cDNA using LunaScript® RT SuperMix Kit (cat# M3010, New England BioLabs, Ipswich, MA, USA). The synthesized cDNAs were used as templates for amplification

(45) Glycolytic PFKFB3 and Glycogenic UGP2 Axis Regulates Perfusion Recovery in Experimental Hind Limb IschemiaArteriosclerosis, Thrombosis, and Vascular BiologyAugust 1, 2024Olukemi Jaiyesimi, Sivaraman Kuppuswamy, Guangwei Zhang, Sonia Batan, Wenbo Wenbo, Vijay C. Gantaamount of RNA was used to prepare cDNA using LunaScript® RT SuperMix (New England BioLabs, Cat no: M3010L) according to manufacturer’s conditions. qPCR was performed by Taqman probes using Luna Universal Probe qPCR Master Mix

(46) Glycolytic PFKFB3 and Glycogenic UGP2 Axis Regulates Perfusion Recovery in Experimental Hind Limb IschemiaArteriosclerosis, thrombosis, and vascular biologyAugust 1, 2024Jaiyesimi O, Kuppuswamy S, Zhang G, Batan S, Zhi W, Ganta VC.amount of RNA was used to prepare cDNA using LunaScript® RT SuperMix (New England BioLabs, Cat no: M3010L) according to manufacturer’s conditions. qPCR was performed by Taqman probes using Luna Universal Probe qPCR Master Mix

(47) Transcriptomic analysis reveals regulation of adipogenesis via long non-coding RNA, alternative splicing, and alternative polyadenylationScientific ReportsJuly 23, 2024Salwa Mohd Mostafa, Luyang Wang, Bin Tian, Joel Graber, Claire Mooretranscription of the RNA samples to cDNA using using NEB LunaScript RT SuperMix kit (New England Biolabs, M3010L) followed by qPCR using NEB Luna Universal qPCR Master Mix (New England Biolabs, M3003L) and primers

(48) Genomic and single-cell characterization of patient-derived tumor organoid models of head and neck squamous cell carcinomabioRxiv : the preprint server for biologyJuly 2, 2024Um JH, Zheng Y, Mao Q, Nam C, Zhao H, Koh YW, Shin SJ, Park YM, Lin DC.Cat#70106). cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR) was performed by using the Powerup™ SYBR Green Master Mix (Thermo Fisher Scientific

(49) ALKBH5-mediated m(6)A demethylation of Runx2 mRNA promotes extracellular matrix degradation and intervertebral disc degenerationCell & bioscienceJune 14, 2024Lei Y, Zhan E, Chen C, Hu Y, Lv Z, He Q, Wang X, Li X, Zhang F.complementary DNA (cDNA) using the LunaScript RT SuperMix Kit (New England Biolabs, Shanghai, China; #M3010) following the manufacturer’s instructions. The resulting cDNA samples were diluted 50-fold and subjected

(50) Human Endogenous Retroviruses in Breast Cancer: Altered Expression Pattern Implicates Divergent Roles in CarcinogenesisOncologyFebruary 26, 2024Luděk Záveský, Eva Jandáková, Vít Weinberger, Luboš Minář, Milada Kohoutová, Ondřej Slanařwas reverse-transcribed to cDNA using a LunaScript RT SuperMix (New England Biolabs, cat. No. NEB #M3010) after an initial ezDNase (Thermo Fisher Scientific, cat. No. 11766051) treatment. The cDNA was stored

(51) Inhibiting anti-angiogenic VEGF165b activates a miR-17-20a-Calcipressin-3 pathway that revascularizes ischemic muscle in peripheral artery diseaseCommunications MedicineJanuary 5, 2024Sonia Batan, Sivaraman Kuppuswamy, Madison Wood, Meghana Reddy, Brian Annex, Vijay Gantaamount of RNA was used to prepare cDNA using LunaScript® RT SuperMix (New England BioLabs, Cat no: M3010L) according to the manufacturer’s conditions. qPCR was performed by TaqMan primer probes using Luna Universal Probe qPCR Master Mix

(52) Identification of Genomic Variants of SARS-CoV-2 Using Nanopore SequencingMedicina (Kaunas)December 15, 2022Ionut Dragos Capraru, Mirabela Romanescu, Flavia Medana Anghel, Cristian Oancea, Catalin Marian, Ioan Ovidiu Sirbu, Aimee Rodica Chis, Paula Diana CiordasSequencing Kit 109 (SQK-LSK109, ONT, Oxford, UK). For cDNA synthesis, 2 uL of LunaScript RT SuperMix (M3010, NEB, Ipswich, MA, USA) was mixed with 8 uL of viral RNA, incubated 2 min at 25 °C, 10 min at 55 °C,

(53) Thymidine rescues ATR kinase inhibitor-induced deoxyuridine contamination in genomic DNA, cell death, and interferon-α/β expressionCell reportsSeptember 20, 2022Sugitani N, Vendetti FP, Cipriano AJ, Pandya P, Deppas JJ, Moiseeva TN, Schamus-Haynes S, Wang Y, Palmer D, Osmanbeyoglu HU, et al.(Thermo). Total RNA (typically 100 ng) was reverse transcribed using the Lunascript RT master mix (NEB, M3010L) and diluted 10-fold. Each PCR reaction was run as technical duplicates using 5 μL of Luna Universal qPCR master mix

(54) High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant DeterminationInternational Journal of Molecular SciencesMarch 17, 2022Yu-Chieh Liao, Feng-Jui Chen, Min-Chieh Chuang, Han-Chieh Wu, Wan-Chen Ji, Guann-Yi Yu, Tsi-Shu HuangHospital. cDNA synthesis was performed on the extracted RNA using LunaScript® RT SuperMix Kit (cat# M3010, New England BioLabs, Ipswich, MA, USA) followed by our proposed multiplex PCR using 12.5 µL of PCR

(55) Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene EditingCellsJanuary 13, 2022Nouri P, Zimmer A, Brüggemann S, Friedrich R, Kühn R, Prakash N.New England Biolabs) and 1 µg of total RNA was reverse transcribed using LunaScript® RT SuperMix Kit (# M3010L; New England Biolabs), following the manufacturers’ instructions. PCR reactions were set up in duplicates

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LunaScript RT SuperMix

LunaScript RT SuperMix from New England Biolabs

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LunaScript RT SuperMix from New England Biolabs

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