LunaScript RT SuperMix M3010L from New England Biolabs

Product Specs
ItemLunaScript RT SuperMix
CompanyNew England Biolabs
Price $403.00 Inquire
Catalog NumberM3010L
Temperature-20 C
ApplicationsDNA Analysis, RT-PCR
Quantity100 reactions

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) STING induces ZBP1-mediated necroptosis independently of TNFR1 and FADDNatureNovember 1, 2025Kelepouras K, Saggau J, Bonasera D, Kiefer C, Locci F, Rakhsh-Khorshid H, Grauvogel L, Varanda AB, Peifer M, Loricchio E, et al.according to manufacturer’s instructions. RT–qPCR cDNA was prepared using the Lunascript RT SuperMix kit (M3010, New England Biolabs). RT–qPCR was performed using Luna Universal qPCR Master Mix (M3003, New England Biolabs

(22) RNA G‐Quadruplex RIBOTAC‐Mediated Targeted Degradation of lncRNA TERRAAdvanced ScienceOctober 6, 2025Elias Khaskia, Dipak Dahatonde, Raphael I. Benhamouwas performed on 1 µg of total RNA using the LunaScript RT SuperMix Kit (New England BioLabs, cat #M3010), following the manufacturer's instructions. Approximately 30 ng of cDNA was used for each qPCR reaction,

(23) Opposing lineage specifiers induce a protumor hybrid identity state in lung adenocarcinomaGenes & DevelopmentSeptember 2, 2025Gabriela Fort, Henry Arnold, Soledad A. Camolotto, Kayla O'Toole, Rushmeen Tariq, Anna Waters, Katherine Gillis, Eric L. Snydermanufacturer's specifications. cDNA was synthesized from TRIzol-extracted RNA using LunaScript RT SuperMix (NEB M3010) according to the manufacturer's specifications. qPCR was performed on cDNA using Luna Universal Probe qPCR master mix

(24) Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cellsNature communicationsAugust 27, 2025Kowar A, Becker JP, Del Pizzo R, Tang Z, Champagne J, Wellach K, Samimi K, Galindo-Albarrán A, Körner PR, Montenegro Navarro J, et al.Quantitative real-time PCR A total of 500 ng of RNA was reverse-transcribed using LunaScript RT Supermix (NEB, M3010L). Quantitative real-time PCR was subsequently performed with Luna Universal qPCR Mix (NEB, M3003X).

(25) Evaluating T1/T2 Relaxometry with OCRA Tabletop MRI System in Fresh Clinical Samples: Preliminary Insights into ZEB1-Associated Tissue CharacteristicsTechnol Cancer Res TreatAugust 26, 2025Ahmed Y. Sanin, Marcus Prier, Thomas Wartmann, Christian Siba, Katrin Hippe, Maciej Pech, Roland S. Croner, Oliver Speck, Ulf D. Kahlert, Georg Roseadhering to manufacturer's instructions. RNA was reverse-transcribed using LunaScript® RT SuperMix (NEB, #M3010L). ZEB1 mRNA expression was quantified via Luna Universal qPCR Master Mix (NEB) with primers (Microsynth

(26) ZEB1 stratifies the response to Sorafenib and Mdivi-1 combination therapy in hepatocellular carcinomaSci RepAugust 19, 2025H. Freudenstein, M. Strecker, S. Gylstorff, W. Shi, M. Boettcher, S. Medunjanin, C. Catapano, C. Siba, C. Wex, T. Wartmann, et al.transcription was performed using 1 µg of RNA and the New England BioLabs LunaScript® RT Super Mix (cat. No. M3010) according to manufacturer’s instruction. Primers used in this study can be found in Supplementary Table

(27) Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytesCurrent BiologyAugust 4, 2025Philip S. Goff, Shyamal Patel, Dawn C. Harper, Tom Carter, Michael S. Marks, Elena V. Sviderskayaassays RNeasy Plus Mini Kit,Qiagen,Cat. # 74134 LunaScript RT SuperMix Kit,New England Biolabs,Cat. # M3010 Luna Universal qPCR Master Mix,New England Biolabs,Cat. # M3003 ,, Experimental models: Cell lines,Experimental models

(28) Reconciling competing models on the roles of condensates and soluble complexes in transcription factor functionMolecular CellJuly 17, 2025Anne Bremer, Walter H. Lang, Ryan P. Kempen, Kumari Sweta, Aaron B. Taylor, Madeleine B. Borgia, Aseem Z. Ansari, Tanja MittagMix,NEB,Cat# E2621 BP Clonase™ II Enzyme Mix,Invitrogen,Cat#11789020 Luna Script® RT Super Mix 5x,NEB,Cat# M3010 2x Luna® Universal qPCR Master Mix,NEB,Cat# M3003 Dynabeads™ Antibody Coupling Kit,Invitrogen,Cat#14311D Deposited

(29) Genomic and Single-Cell Analyses Characterize Patient-Derived Tumor Organoids to Enable Personalized Therapy for Head and Neck Squamous Cell CarcinomaCancer ResJuly 15, 2025Jung Hyun Um, Yueyuan Zheng, Qiong Mao, Chehyun Nam, Hua Zhao, Yoon Woo Koh, Su-Jin Shin, Young Min Park, De-Chen LinCat#70106). cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR) was performed by using the Powerup™ SYBR Green Master Mix (Thermo Fisher Scientific

(30) Genomic and Single-Cell Analyses Characterize Patient-Derived Tumor Organoids to Enable Personalized Therapy for Head and Neck Squamous Cell CarcinomaCancer ResearchJuly 15, 2025Jung Hyun Um, Yueyuan Zheng, Qiong Mao, Chehyun Nam, Hua Zhao, Yoon Woo Koh, Su-Jin Shin, Young Min Park, De-Chen LinCat#70106). cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR) was performed by using the Powerup™ SYBR Green Master Mix (Thermo Fisher Scientific

(31) Incidence of Human Sapovirus Outbreaks in Beijing, China, 2014–2021: Predominance of Genotypes GII.3 and GI.2Emerging Microbes & InfectionsJune 23, 2025Lingyu Shen, Fan Yu, Hanqiu Yan, Baiwei Liu, Weihong Li, Yu Wang, Yi Tian, Lei Jia, Peng Yang, Quanyi Wang, et al.respectively. Reverse transcription was performed using the LunaScript® RT SuperMix Kit (Cat. No. NEB #M3010; New England Biolabs, Massachusetts, USA) in a 10 μL reaction volume, with odd-numbered primers used

(32) Pancreatic cancer cachexia is mediated by PTHrP-driven disruption of adipose de novo lipogenesisbioRxivJune 7, 2025Nikita Bhalerao, Yamini Ogoti, Jessica Peura, Calvin Johnson, Qingbo Chen, Ekaterina D. Korobkina, Faith N. Keller, Maximilian Wengyn, Robert J. Norgard, Claire Shamber, et al.cDNA was prepared using 100ng-1000ng RNA using the New England Biolabs LunaScript RT supermix (Cat# M3010L). Quantitative PCR was setup using Luna Universal qPCR Master Mix (Cat #M3003E). The following primers

(33) Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironmentNature communicationsMay 19, 2025Aviles-Huerta D, Del Pizzo R, Kowar A, Baig AH, Palazzo G, Stepanova E, Amaya Ramirez CC, D'Agostino S, Ratto E, Pechincha C, et al.(Thermo Fisher). 600 ng of total RNA was reversed transcribed using LunaScript RT Supermix (NEB; cat. M3010L) and quantitative real-time PCR was performed using Luna Universal qPCR Mix (NEB; cat. M3003X). Ct values

(34) Neonatal gut microbiota succession in mice mapped by maturation, site, injury, and single immunoglobulin interleukin-1 related receptor genotypeiScienceApril 18, 2025Shahid Umar, Wei Yu, Hao Xuan, Ishfaq Ahmed, Cuncong Zhong, Michael Morowitz, Mathew Brian Rogers, Mark Ivan Attard, Venkatesh Sampathproteins" ,, Esbilac canine milk replacer,PetAg,N/A TRiZol,ThermoFisher,15596018 cDNA reverse transcription kit,NEB,M3010 FLUORO-GEL,Electron Microscopy Science,1798510 ,, Critical commercial assays,Critical commercial

(35) Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinomaMolecular Systems BiologyApril 15, 2025Fuyuan Li, Shangzi Wang, Lian Chen, Ning Jiang, Xingdong Chen, Jin Li,New England Biolabs,M0318L Luna® Universal qPCR Master Mix,New England Biolabs,M3003E LunaScript RT SuperMix,New England Biolabs,M3010L Q5® Hot Start High-Fidelity DNA,New England Biolabs,M0493L Q5 Site-Directed Mutagenesis kit,New England Biolabs

(36) Synergistic Effects of Photobiomodulation and Differentiation Inducers on Osteogenic Differentiation of Adipose-Derived Stem Cells in Three-Dimensional CultureInternational Journal of Molecular SciencesDecember 12, 2024Daniella Da Silva, Anine Crous, Heidi Abrahamsefor cDNA synthesis with the LunaScript® RT SuperMix Kit (New England Biolabs, Pretoria, South Africa, M3010) and a universal reverse primer. The target genes, listed in Table 3 (Inqaba Biotechnical Industries,

(37) Genetically-stable engineered optogenetic gene switches modulate spatial cell morphogenesis in two- and three-dimensional tissue culturesNature CommunicationsDecember 2, 2024Hannes M. Beyer, Sant Kumar, Marius Nieke, Carroll M. C. Diehl, Kun Tang, Sara Shumka, Cha San Koh, Christian Fleck, Jamie A. Davies, Mustafa Khammash, et al.740984.50) and converted into cDNA using the LunaScript RT SuperMix Kit (NEB, Ipswich, MA, US, cat. no. M3010L). Mouse Wnt3a was probed using the oligonucleotides oDD337 (5’-CATGCACCTCAAGTGCAAATGC) and oDD338 (5’-TGAGGAAATCCCCGATGGTG)

(38) Junctophilin-2 is a double-stranded RNA-binding protein that regulates cardiomyocyte-autonomous innate immune responseBiochemical and Biophysical Research CommunicationsNovember 1, 2024Yun Shi, Seyedsaber Mirabdali, Stefan W. Vetter, Ang GuoKit (Qiagen #74204). Reverse transcription of mRNA was performed using LunaScript® RT SuperMix (NEB M3010). qPCR was conducted using LiQuant Universal qPCR Master Mix (Lifesct M0016) with a BioRad qPCR machine.

(39) Synthetic Antiangiogenic Vascular Endothelial Growth Factor-A Splice Variant Revascularizes Ischemic Muscle in Peripheral Artery DiseaseJournal of the American Heart AssociationOctober 15, 2024Raja A, Ganta V.equal amount of RNA was used to prepare cDNA using LunaScript RT SuperMix (New England BioLabs, Cat no: M3010L) according to the manufacturer's conditions. quantitative polymerase chain reaction (qPCR) was performed

(40) MED12 and CDK8/19 Modulate Androgen Receptor Activity and Enzalutamide Response in Prostate CancerEndocrinologySeptember 10, 2024Chiara Andolfi, Caterina Bartolini, Elisa Morales, Büşra Gündoğdu, Martin Puhr, Juan Guzman, Sven Wach, Helge Taubert, Achim Aigner, Iris E Eder, et al.kit (Zymo, #R2132). cDNA was synthesized using the LunaScript® RT SuperMix Kit (New England Biolabs, #M3010X), and gene expression was quantified by quantitative PCR (qPCR) using the Luna® Universal qPCR Master Mix

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LunaScript RT SuperMix

LunaScript RT SuperMix from New England Biolabs

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LunaScript RT SuperMix from New England Biolabs

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