DuoSet ELISA Ancillary Reagent Kit 2 DY008B from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDuoSet ELISA Ancillary Reagent Kit 2
CompanyR&D Systems, a Bio-Techne Brand
Price $365.00Supplier Page View Company Product Page
Catalog NumberDY008B
ApplicationsELISA
Quantity1 Kit (5 x 96-well Plates) (also 1 Kit (5 x 384-well Plates))
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The p.(Leu97Ile) variant expands the genetic landscape of NEFL-associated Charcot-Marie-tooth neuropathiesHum Mol GenetApril 22, 2026Menekse Oeztuerk, Sara Walli, David Muhmann, Catherine Choueiri, Vera Dobelmann, Angela Abicht, Barbara Leube, Ulrike Schara-Schmidt, Sven G Meuth, Rita Horvath, et al.enzyme-linked immunosorbent assay (ELISA). The following commercial kits were employed: NCAM1 (Cat. DY2408, DY008B, R&D Systems), GDF15 (Cat. DGD150, R&D Systems), FGF21 (Cat. RD191108200R, BioVendor), Periaxin (Cat.

(2) ZBP1 mediated restriction of Toxoplasma gondii in both the early acute and chronic brain stages of infectionImmunohorizonsApril 17, 2026Lydia Anne Sibley, Isaac William Babcock, Naa Dedee Amadi, Anne Elise Marchildon, Abigail Grace Kelly, Sydney Ann Labuzan, John Robert Lukens, Tajie Heartsong HarrisMouse IL-18 [R&D Systems; Cat# DY7625-05]) with the DuoSet Ancillary Reagent Kit 2 (R&D Systems; Cat# DY008B). Parasite burden assessments Parasite burden was measured by real-time polymerase chain reaction

(3) Adenovirus phagocytosis by neutrophils triggers a pro-inflammatory responsePLoS PathogApril 6, 2026Salomé Laurans, Soizic Huerre, Olivier Dellis, Céline Férard, Hadrien Jalaber, Clément Vanbergue, Emilie Brun, Rémy Jelin, Oliver Nüsse, Karim Benihoud, et al.supernatants of PMNs was determined using the Human CXCL8 ELISA kit (R&DSystems BioTechne, DY208–05 and DY008B), following the supplier’s instructions. Binding assay A488-labeled Ads were diluted in Hanks’ balanced

(4) Global molecular landscape of early MASLD progression in human obesityeLifeMarch 23, 2026Qing Zhao, William De Nardo, Ruoyu Wang, Yi Zhong, Umur Keles, Gabriele Sakalauskaite, Li Na Zhao, Huiyi Tay, Sonia Youhanna, Mengchao Yan, et al.,R&D Systems,Cat. #: DY6220-05, Commercial assay or kit,DuoSet ELISA Ancillary Reagent Kit 2,R&D Systems,Cat. #: DY008B, "Chemical compound, drug",NSC23766,MedChemExpress,Cat. #: HY-15723, "Chemical compound, drug",ML141,MedChemExpress,Cat.

(5) Plasma Extracellular Vesicle Modulate Immune Cell Transcriptional Responses Following Acute Myocardial InfarctioniScienceMarch 20, 2026Lewis Timms, Raphaella Ridley, Adam Braithwaite, Charlotte Hooper, Tanvir Rafe, Carolin Lewe, Sebastian Lemke, Divya Ganesh, Zeyu Chen, Tafadzwa Kufazvinei, et al.IL-6,Biotechne,DY206 ELISA TNF-α,Biotechne,DY210 DuoSet ELISA Ancillary Reagent Kit 2 (5 96 well plates),Biotechne,DY008B GeneChip miRNA 4.0 Arrays,Thermofisher Scientific,902411 ,, Deposited data,Deposited data,Deposited

(6) Rejuvenation of mesenchymal stromal cells via partial reprogramming enables scalable generation of transcriptionally diverse MSC librariesStem Cell Research & TherapyMarch 20, 2026Haochen Tu, Aoi Hosaka, Genki Hichiwa, Yayan Wang, Kanako Kazuki, Toshiaki Tabata, Mitsuhiko Osaki, Yuji Nakayama, Iori Kanazawa, Kazuhisa Honma, et al.instructions. Indoleamine 2,3-dioxygenase 1 (IDO1) levels were quantified using ELISA kits DY6030B-05 and DY008B (R&D Systems, Minneapolis, MN, USA). Absorbance was measured using a microplate reader, and protein

(7) TRPC6-Mediated Ca2+ Influx Activates MAPK and NFκB Signaling and Elicits Pro-Inflammatory and Catabolic Responses in Human Intervertebral Disc CellsCellsMarch 17, 2026Janitri Venkatachala Babu, Varun Puvanesarajah, Addisu Mesfin, Jonathan P. Japa, Kevin Yoon, Mark Ehioghae, Michael G. Schrlau, Laura S. Stone, Wolfgang Hitzl, Karin Wuertz-Kozakcentrifuged to remove debris and analyzed using human DuoSet ELISA kits (R&D Systems, Minneapolis, MN, USA; DY008B) following the manufacturer’s instructions. The analytes included IL-6 (DY206), IL-8 (DY208), MMP-1

(8) IL1β+ macrophages promote intestinal fibrosis in Crohn’s disease by secreting AREG to activate PI16+ fibroblastsJournal of Translational MedicineMarch 14, 2026Hanlin Xie, Xiaofeng Wen, Tao Ma, Lin Wang, Yanxu Guo, Guanzhan Liang, Jing Chen, Ruibing Li, Yuxi Pan, Zhenyu Xian, et al.curves generated using ELISACalc software. The ELISA kits used in this study were as follows: DY262, DY008B, DY008C, DY999B, DY999, DY999B-250 (R&D Systems). Primary human intestinal fibroblasts isolation Three

(9) Elevated Activin-A serum levels in patients with acute peripartum cardiomyopathy and during left ventricular recoveryESC Heart FailureMarch 3, 2026Thomas Gausepohl, Tobias Jonathan Pfeffer, Tobias König, Dominik Berliner, Martina Kasten, Jason Roh, Denise Hilfiker-Kleiner, Johann Bauersachs, Melanie Ricke-HochActivin-A serum concentrations were determined using a commercial ELISA kit (R&D systems, DY338 and DY008B),7 both according to the manufacturer's protocol. Laboratory workup was performed as part of the routine

(10) Antiviral innate immunity induces alpha synuclein phosphorylation at serine129 in neurons independent of aggregationnpj Parkinson's DiseaseFebruary 23, 2026Dustin L. Heiden, Camille Merrick, Robert C. Evans, Alexander J. Rajic, Madeline Slater, Ravi Mahalingam, Vicki Traina-Dorge, Jennifer N. Berger, Leslie J. Berg, J. David BeckhamBioscience, 42400), IFN-gamma (Thermo Fisher, BMS606-2), and IFN-lambda2/3 (R&D Biotechne, DY1789B, DY008B) using commercial kits as per the manufacturers’ instructions. Samples were run in triplicate. Standards

(11) Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric Immunoglobulin Receptor (PIGR)Clinical Cancer ResearchFebruary 17, 2026Brianna M. Krusen, Phyllis A. Gimotty, Greg Donahue, Jacob E. Till, Melinda Yin, Erin E. Carlson, William R. Bamlet, Erica L. Carpenter, Shounak Majumder, Ann L. Oberg, et al.prepared following manufacturer instructions using DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems Cat. #DY008B) by adding 100 uL of diluted capture antibody to each well and incubating at room temperature overnight

(12) PCSK5M452I Is a Recessive Hypomorph Exclusive to MCF10DCIS.com CellsMolecular Cancer ResearchFebruary 6, 2026Taylor Marohl, Kristen A. Atkins, Lixin Wang, Kevin A. JanesGDF11 ELISA (R&D Systems, DY1958). If needed, samples were pre-diluted in Reagent Diluent (R&D Systems, DY008B) to remain within the standard range of the assay (31–2000 pg/mL). The calibration curve was modeled

(13) Secreted GPNMB enhances uptake of fibrillar alpha-synuclein in a non-cell-autonomous process that can be blocked by anti-GPNMB antibodiesmedRxivFebruary 1, 2026Marc Carceles-Cordon, Eliza M. Brody, Masen L. Boucher, Michael D. Gallagher, Robert T. Skrinak, Travis L. Unger, Cooper K. Penner, Adama J. Berndt, Sromona Das, Katie Lam, et al.kit (R&D Systems, #DY2550) in conjunction with the DuoSet ELISA Ancillary Reagent Kit (R&D Systems, #DY008B). CM from PiggyBac-GPNMB HEK293 cells was diluted 1:500, while CM from iMicroglia was diluted 1:25.

(14) Engineered macroporous gelatin scaffolds enhance lymph node fibroblastic reticular cell identity and enable diabetogenic T cell immunomodulationBiomaterialsJanuary 1, 2026Leonor N. Teles, Logan A. Beatty, Ana V. Hernandez, Marvin A. Mendoza, Zachary M. Wilkes, Vivien P. Dominick, Michelle T. Argy Telias, Benjamin Miller, Chun-Yuh Huang, Camillo Bechi Bechi Genzano, et al.measured using a mouse TGF-β2 duo-set ELISA kit (DY7346–05) and supplementary ancillary reagent kit (DY008B) per manufacturer’s instructions (R&D Systems, Minneapolis, MN). Results were reported by constructing

(15) A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain BarrierASN NeuroDecember 31, 2025Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutzcellular debris before the clarified supernatant was used for ELISA (R&D Systems DY466-05, R&D Systems DY008B). Colorimetric development was detected with a Beckman Coulter DTX880 plate reader. CXCL10 concentration

(16) Intracellular IL1α in Peritumoral Monocytes Induces IL8 Production and Inhibits Mitophagy to Promote Stemness and Metastasis of Hepatocellular CarcinomaCancer researchNovember 3, 2025Ruan YH, Ning WR, Huang AQ, Jiang D, Zhu BX, Liu XC, Gong JL, Huang K, Kuang DM, Wu Y, et al.

(17) High Fat and Sugar Diet Increases Enteric cDC1 and Oral Antigen-Specific TregsJ Immunol ResOctober 28, 2025K. Fischer, O. Horno, B. Raposo, I. Godinho, H. Ribeiro, H. Veiga-Fernandes, M. Martínez-LópezFisher,Mm01178820_m1 Elisa,Elisa,Elisa 2‐Mercaptoethanol,Gibco,31350‐010 BCA Protein Assay Kits,Pierce,23225 DuoSet ELISA Ancillary Reagent Kit 2,Biotechne,DY008B EDTA,Corning,46‐034‐Cl EGTA,Sigma–Aldrich,E3889 Mouse FLT3L DuoSet ELISA,Biotechne,DY427 NaF,Sigma–Aldrich,201154 PMSF,Sigma–Aldrich,P7626 Protease

(18) Role of conventional dendritic cells in schistosomiasis-induced pulmonary hypertensionClinical ScienceOctober 22, 2025Claudia Mickael, Rahul Kumar, Dara C. Fonseca Balladares, Kevin Nolan, Michael H. Lee, Biruk Kassa, Thais C.F Menezes, Anthony Lau-Xiao, Ajaypal Sahota, Linda Sanders, et al.quantification of IL-4 and IL-13 (R&D Systems, Minneapolis, MN; DY404-05 and DY413-05, respectively, with DY008B ancillary reagents) using whole lung lysates from unexposed and Schistosoma-exposed mice, following

(19) The Contribution of CD26-Negative Fibroblasts to Endometrial ScarringBiomoleculesOctober 10, 2025Muhammad Assad Riaz, Clara Marie Pecher, Franziska Louisa Kary, Jane Bosibori Maoga, Raimund Dietze, Felix Zeppernick, Ivo Meinhold-Heerlein, Lutz KonradDuoSet ELISA (cat-no. DY6220, range 31.2–2000 pg/mL) and DuoSet ELISA ancillary reagent kit 2, (cat-no. DY008B). All ELISAs are from R&D Systems. 2.5. Cell Culture and Experimental Protocol All experiments were

(20) Dynamic profiles of 25 serum biomarkers in acute myocardial infarctionFrontiers in Cardiovascular MedicineSeptember 25, 2025Xuefeng Ai, Yuanyuan Wang, Mingwei Jiang, Qianyu Han, Hongli Geng, Cheng Wang, Jirui Chen, Lei Xue, Yuxiang Jinassay Serum levels of 25 biomarkers (α-SMA, ANPEP, BNP, etc.) were quantified using commercial ELISA kits (DY008B, R&D systems) according to the manufacturer's protocols. Briefly, serum samples and standards were added

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DuoSet ELISA Ancillary Reagent Kit 2 from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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DuoSet ELISA Ancillary Reagent Kit 2 from R&D Systems, a Bio-Techne Brand

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